Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)

Detalhes bibliográficos
Autor(a) principal: Bardella, Vanessa Bellini
Data de Publicação: 2016
Outros Autores: Pita, Sebastián, Vanzela, André Luis Laforga, Galvão, Cleber, Panzera, Francisco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17961
Resumo: Universidade Estadual Paulista. Instituto de Biociências. Departamento de Biologia. Rio Claro, SP, Brasil.
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spelling Bardella, Vanessa BelliniPita, SebastiánVanzela, André Luis LaforgaGalvão, CleberPanzera, Francisco2017-03-07T15:15:15Z2017-03-07T15:15:15Z2016BARDELLA, Vanessa Bellini; et al. Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae). Mem Inst Oswaldo Cruz, v.111, n.10, p.614-624, Oct. 2016.1678-8080https://www.arca.fiocruz.br/handle/icict/1796110.1590/S1984-29612016065engFundação Oswaldo Cruz. Instituto Oswaldo CruzTriatominaeDNA repetitivoHeteropteraCromossomos holocêntricosHeteropteraTriatominaeholocentric chromosomesCMA/DAPI bandingrepetitive DNAHeterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual Paulista. Instituto de Biociências. Departamento de Biologia. Rio Claro, SP, Brasil.Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Biologia Geral. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Rio de Janeiro, RJ, Brasil.Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.The subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17961/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALcleber_galvao_etal_IOC_2016.pdfcleber_galvao_etal_IOC_2016.pdfapplication/pdf2748793https://www.arca.fiocruz.br/bitstream/icict/17961/2/cleber_galvao_etal_IOC_2016.pdf860f52373d5ea4435f440b97e2e4f1aeMD52TEXTcleber_galvao_etal_IOC_2016.pdf.txtcleber_galvao_etal_IOC_2016.pdf.txtExtracted texttext/plain45145https://www.arca.fiocruz.br/bitstream/icict/17961/3/cleber_galvao_etal_IOC_2016.pdf.txtbecd11abde41b69587038a68336175b5MD53icict/179612018-08-15 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dc.title.pt_BR.fl_str_mv Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
title Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
spellingShingle Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
Bardella, Vanessa Bellini
Triatominae
DNA repetitivo
Heteroptera
Cromossomos holocêntricos
Heteroptera
Triatominae
holocentric chromosomes
CMA/DAPI banding
repetitive DNA
title_short Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
title_full Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
title_fullStr Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
title_full_unstemmed Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
title_sort Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
author Bardella, Vanessa Bellini
author_facet Bardella, Vanessa Bellini
Pita, Sebastián
Vanzela, André Luis Laforga
Galvão, Cleber
Panzera, Francisco
author_role author
author2 Pita, Sebastián
Vanzela, André Luis Laforga
Galvão, Cleber
Panzera, Francisco
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Bardella, Vanessa Bellini
Pita, Sebastián
Vanzela, André Luis Laforga
Galvão, Cleber
Panzera, Francisco
dc.subject.other.pt_BR.fl_str_mv Triatominae
DNA repetitivo
Heteroptera
Cromossomos holocêntricos
topic Triatominae
DNA repetitivo
Heteroptera
Cromossomos holocêntricos
Heteroptera
Triatominae
holocentric chromosomes
CMA/DAPI banding
repetitive DNA
dc.subject.en.pt_BR.fl_str_mv Heteroptera
Triatominae
holocentric chromosomes
CMA/DAPI banding
repetitive DNA
description Universidade Estadual Paulista. Instituto de Biociências. Departamento de Biologia. Rio Claro, SP, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-03-07T15:15:15Z
dc.date.available.fl_str_mv 2017-03-07T15:15:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARDELLA, Vanessa Bellini; et al. Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae). Mem Inst Oswaldo Cruz, v.111, n.10, p.614-624, Oct. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17961
dc.identifier.issn.pt_BR.fl_str_mv 1678-8080
dc.identifier.doi.none.fl_str_mv 10.1590/S1984-29612016065
identifier_str_mv BARDELLA, Vanessa Bellini; et al. Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae). Mem Inst Oswaldo Cruz, v.111, n.10, p.614-624, Oct. 2016.
1678-8080
10.1590/S1984-29612016065
url https://www.arca.fiocruz.br/handle/icict/17961
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/17961/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/17961/2/cleber_galvao_etal_IOC_2016.pdf
https://www.arca.fiocruz.br/bitstream/icict/17961/3/cleber_galvao_etal_IOC_2016.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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