Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0074-02760160044 http://hdl.handle.net/11449/162063 |
Resumo: | The subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae. |
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Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)HeteropteraTriatominaeholocentric chromosomesCMA/DAPI bandingrepetitive DNAThe subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae.Udelar (CSIC) (Uruguay)PEDECIBA (Uruguay)ANII (Uruguay)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Inst Biociencias, Dept Biol, Rio Claro, SP, BrazilUniv la Republ, Fac Ciencias, Secc Genet Evolut, Montevideo, UruguayUniv Estadual Londrina, Ctr Ciencias Biol, Dept Biol Geral, Londrina, PR, BrazilInst Oswaldo Cruz, Lab Nacl & Int Referencia Taxon Triatomineos, Rio De Janeiro, RJ, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Biol, Rio Claro, SP, BrazilUdelar (CSIC) (Uruguay): 370Fundaco Oswaldo CruzUniversidade Estadual Paulista (Unesp)Univ la RepublUniversidade Estadual de Londrina (UEL)Inst Oswaldo CruzBardella, Vanessa Bellini [UNESP]Pita, SebastianLaforga Vanzela, Andre LuisGalvao, CleberPanzera, Francisco2018-11-26T17:06:43Z2018-11-26T17:06:43Z2016-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article614-624application/pdfhttp://dx.doi.org/10.1590/0074-02760160044Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 111, n. 10, p. 614-624, 2016.0074-0276http://hdl.handle.net/11449/16206310.1590/0074-02760160044S0074-02762016001000614WOS:000385514100003S0074-02762016001000614.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemorias Do Instituto Oswaldo Cruz1,172info:eu-repo/semantics/openAccess2024-01-10T06:24:50Zoai:repositorio.unesp.br:11449/162063Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:36:37.936243Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
title |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
spellingShingle |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) Bardella, Vanessa Bellini [UNESP] Heteroptera Triatominae holocentric chromosomes CMA/DAPI banding repetitive DNA |
title_short |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
title_full |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
title_fullStr |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
title_full_unstemmed |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
title_sort |
Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae) |
author |
Bardella, Vanessa Bellini [UNESP] |
author_facet |
Bardella, Vanessa Bellini [UNESP] Pita, Sebastian Laforga Vanzela, Andre Luis Galvao, Cleber Panzera, Francisco |
author_role |
author |
author2 |
Pita, Sebastian Laforga Vanzela, Andre Luis Galvao, Cleber Panzera, Francisco |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ la Republ Universidade Estadual de Londrina (UEL) Inst Oswaldo Cruz |
dc.contributor.author.fl_str_mv |
Bardella, Vanessa Bellini [UNESP] Pita, Sebastian Laforga Vanzela, Andre Luis Galvao, Cleber Panzera, Francisco |
dc.subject.por.fl_str_mv |
Heteroptera Triatominae holocentric chromosomes CMA/DAPI banding repetitive DNA |
topic |
Heteroptera Triatominae holocentric chromosomes CMA/DAPI banding repetitive DNA |
description |
The subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 2018-11-26T17:06:43Z 2018-11-26T17:06:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0074-02760160044 Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 111, n. 10, p. 614-624, 2016. 0074-0276 http://hdl.handle.net/11449/162063 10.1590/0074-02760160044 S0074-02762016001000614 WOS:000385514100003 S0074-02762016001000614.pdf |
url |
http://dx.doi.org/10.1590/0074-02760160044 http://hdl.handle.net/11449/162063 |
identifier_str_mv |
Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 111, n. 10, p. 614-624, 2016. 0074-0276 10.1590/0074-02760160044 S0074-02762016001000614 WOS:000385514100003 S0074-02762016001000614.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memorias Do Instituto Oswaldo Cruz 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
614-624 application/pdf |
dc.publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129442596257792 |