Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)

Detalhes bibliográficos
Autor(a) principal: Bardella, Vanessa Bellini [UNESP]
Data de Publicação: 2016
Outros Autores: Pita, Sebastian, Laforga Vanzela, Andre Luis, Galvao, Cleber, Panzera, Francisco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0074-02760160044
http://hdl.handle.net/11449/162063
Resumo: The subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae.
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spelling Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)HeteropteraTriatominaeholocentric chromosomesCMA/DAPI bandingrepetitive DNAThe subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae.Udelar (CSIC) (Uruguay)PEDECIBA (Uruguay)ANII (Uruguay)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Inst Biociencias, Dept Biol, Rio Claro, SP, BrazilUniv la Republ, Fac Ciencias, Secc Genet Evolut, Montevideo, UruguayUniv Estadual Londrina, Ctr Ciencias Biol, Dept Biol Geral, Londrina, PR, BrazilInst Oswaldo Cruz, Lab Nacl & Int Referencia Taxon Triatomineos, Rio De Janeiro, RJ, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Biol, Rio Claro, SP, BrazilUdelar (CSIC) (Uruguay): 370Fundaco Oswaldo CruzUniversidade Estadual Paulista (Unesp)Univ la RepublUniversidade Estadual de Londrina (UEL)Inst Oswaldo CruzBardella, Vanessa Bellini [UNESP]Pita, SebastianLaforga Vanzela, Andre LuisGalvao, CleberPanzera, Francisco2018-11-26T17:06:43Z2018-11-26T17:06:43Z2016-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article614-624application/pdfhttp://dx.doi.org/10.1590/0074-02760160044Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 111, n. 10, p. 614-624, 2016.0074-0276http://hdl.handle.net/11449/16206310.1590/0074-02760160044S0074-02762016001000614WOS:000385514100003S0074-02762016001000614.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemorias Do Instituto Oswaldo Cruz1,172info:eu-repo/semantics/openAccess2024-01-10T06:24:50Zoai:repositorio.unesp.br:11449/162063Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:36:37.936243Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
title Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
spellingShingle Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
Bardella, Vanessa Bellini [UNESP]
Heteroptera
Triatominae
holocentric chromosomes
CMA/DAPI banding
repetitive DNA
title_short Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
title_full Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
title_fullStr Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
title_full_unstemmed Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
title_sort Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
author Bardella, Vanessa Bellini [UNESP]
author_facet Bardella, Vanessa Bellini [UNESP]
Pita, Sebastian
Laforga Vanzela, Andre Luis
Galvao, Cleber
Panzera, Francisco
author_role author
author2 Pita, Sebastian
Laforga Vanzela, Andre Luis
Galvao, Cleber
Panzera, Francisco
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ la Republ
Universidade Estadual de Londrina (UEL)
Inst Oswaldo Cruz
dc.contributor.author.fl_str_mv Bardella, Vanessa Bellini [UNESP]
Pita, Sebastian
Laforga Vanzela, Andre Luis
Galvao, Cleber
Panzera, Francisco
dc.subject.por.fl_str_mv Heteroptera
Triatominae
holocentric chromosomes
CMA/DAPI banding
repetitive DNA
topic Heteroptera
Triatominae
holocentric chromosomes
CMA/DAPI banding
repetitive DNA
description The subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-01
2018-11-26T17:06:43Z
2018-11-26T17:06:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0074-02760160044
Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 111, n. 10, p. 614-624, 2016.
0074-0276
http://hdl.handle.net/11449/162063
10.1590/0074-02760160044
S0074-02762016001000614
WOS:000385514100003
S0074-02762016001000614.pdf
url http://dx.doi.org/10.1590/0074-02760160044
http://hdl.handle.net/11449/162063
identifier_str_mv Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 111, n. 10, p. 614-624, 2016.
0074-0276
10.1590/0074-02760160044
S0074-02762016001000614
WOS:000385514100003
S0074-02762016001000614.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memorias Do Instituto Oswaldo Cruz
1,172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 614-624
application/pdf
dc.publisher.none.fl_str_mv Fundaco Oswaldo Cruz
publisher.none.fl_str_mv Fundaco Oswaldo Cruz
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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