Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1002/jcp.29274 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/43025 |
Resumo: | Acesso aberto em 27/08;/2020 - https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29274 |
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Honorato, Jessica R.Hauser-Davis, Rachel A.Saggioro, Enrico M.Correia, Fábio V.Sales Junior, Sidney F.Soares, Lorena O. S.Lima, Leandro da R.Moura Neto, VivaldoLopes, Giselle P de FSpohr, Tania C. L. de S.2020-08-27T12:49:06Z2020-08-27T12:49:06Z2020HONORATO, Jessica R. et al. Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma. Journal of Cellular Physiology, v. 235, p. 3798-3814, 2020.0021-9541https://www.arca.fiocruz.br/handle/icict/4302510.1002/jcp.292741097-4652Acesso aberto em 27/08;/2020 - https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29274Secretaria de Estado de Saúde. Instituto Estadual do Cérebro Paulo Niemeyer. Laboratório de Biomedicina do Cérebro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Programa de Pós‐Graduação em Anatomia Patológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Saneamento e Saúde Ambiental. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Departamento de Ciências Naturais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Programa de Pós‐Graduação em Saúde Pública e Meio Ambiente. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Departamento de Ciências Naturais. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Departamento de Ciências Naturais. Rio de Janeiro, RJ, Brasil.Secretaria de Estado de Saúde. Instituto Estadual do Cérebro Paulo Niemeyer. Laboratório de Biomedicina do Cérebro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Programa de Pós‐Graduação em Anatomia Patológica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Programa de Pós‐Graduação em Anatomia Patológica. Rio de Janeiro, RJ, Brasil / Instituto de Estudos do Mar Almirante Paulo Moreira. Departamento de Biotecnologia Marinha. / Instituto Nacional de Câncer. Coordenação de Pesquisa. Rio de Janeiro, RJ, Brasil.Secretaria de Estado de Saúde. Instituto Estadual do Cérebro Paulo Niemeyer. Laboratório de Biomedicina do Cérebro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Programa de Pós‐Graduação em Anatomia Patológica. Rio de Janeiro, RJ, Brasil.The first-line chemotherapy treatment for Glioblastoma (GBM) - the most aggressive and frequent brain tumor - is temozolomide (TMZ). The Sonic hedgehog (SHH) pathway is involved with GBM tumorigenesis and TMZ chemoresistance. The role of SHH pathway inhibition in the potentiation of TMZ's effects using T98G, U251, and GBM11 cell lines is investigated herein. The combination of GANT-61 and TMZ over 72 hr suggested a synergistic effect. All TMZ-resistant cell lines displayed a significant decrease in cell viability, increased DNA fragmentation and loss of membrane integrity. For T98G cells, G2 /M arrest was observed, while U251 cells presented a significant increase in reactive oxygen species production and catalase activity. All the cell lines presented acidic vesicles formation correlated to Beclin-1 overexpression. The combined treatment also enhanced GLI1 expression, indicating the presence of select resistant cells. The selective inhibition of the SHH pathway potentiated the cytotoxic effect of TMZ, thus becoming a promising in vitro strategy for GBM treatment.engWileyGlioblastomaGANT‐61Ouriço sônicoTemozolomidaGANT‐61GlioblastomaSonic hedgehogTemozolomideRole of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastomainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43025/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJessicaHonorato_RachelDavis_etal_IOC_2020.pdfJessicaHonorato_RachelDavis_etal_IOC_2020.pdfapplication/pdf4199234https://www.arca.fiocruz.br/bitstream/icict/43025/2/JessicaHonorato_RachelDavis_etal_IOC_2020.pdfe314e0f5912b3bbbc8cf1362c11c917bMD52TEXTJessicaHonorato_RachelDavis_etal_IOC_2020.pdf.txtJessicaHonorato_RachelDavis_etal_IOC_2020.pdf.txtExtracted texttext/plain73631https://www.arca.fiocruz.br/bitstream/icict/43025/3/JessicaHonorato_RachelDavis_etal_IOC_2020.pdf.txt0f6655aae587763c922be01f3e31130dMD53icict/430252020-12-01 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| dc.title.pt_BR.fl_str_mv |
Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma |
| title |
Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma |
| spellingShingle |
Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma Honorato, Jessica R. Glioblastoma GANT‐61 Ouriço sônico Temozolomida GANT‐61 Glioblastoma Sonic hedgehog Temozolomide |
| title_short |
Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma |
| title_full |
Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma |
| title_fullStr |
Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma |
| title_full_unstemmed |
Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma |
| title_sort |
Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma |
| author |
Honorato, Jessica R. |
| author_facet |
Honorato, Jessica R. Hauser-Davis, Rachel A. Saggioro, Enrico M. Correia, Fábio V. Sales Junior, Sidney F. Soares, Lorena O. S. Lima, Leandro da R. Moura Neto, Vivaldo Lopes, Giselle P de F Spohr, Tania C. L. de S. |
| author_role |
author |
| author2 |
Hauser-Davis, Rachel A. Saggioro, Enrico M. Correia, Fábio V. Sales Junior, Sidney F. Soares, Lorena O. S. Lima, Leandro da R. Moura Neto, Vivaldo Lopes, Giselle P de F Spohr, Tania C. L. de S. |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Honorato, Jessica R. Hauser-Davis, Rachel A. Saggioro, Enrico M. Correia, Fábio V. Sales Junior, Sidney F. Soares, Lorena O. S. Lima, Leandro da R. Moura Neto, Vivaldo Lopes, Giselle P de F Spohr, Tania C. L. de S. |
| dc.subject.other.pt_BR.fl_str_mv |
Glioblastoma GANT‐61 Ouriço sônico Temozolomida |
| topic |
Glioblastoma GANT‐61 Ouriço sônico Temozolomida GANT‐61 Glioblastoma Sonic hedgehog Temozolomide |
| dc.subject.en.pt_BR.fl_str_mv |
GANT‐61 Glioblastoma Sonic hedgehog Temozolomide |
| description |
Acesso aberto em 27/08;/2020 - https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29274 |
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2020 |
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2020-08-27T12:49:06Z |
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2020-08-27T12:49:06Z |
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2020 |
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info:eu-repo/semantics/article |
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HONORATO, Jessica R. et al. Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma. Journal of Cellular Physiology, v. 235, p. 3798-3814, 2020. |
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https://www.arca.fiocruz.br/handle/icict/43025 |
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0021-9541 |
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10.1002/jcp.29274 |
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1097-4652 |
| identifier_str_mv |
HONORATO, Jessica R. et al. Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma. Journal of Cellular Physiology, v. 235, p. 3798-3814, 2020. 0021-9541 10.1002/jcp.29274 1097-4652 |
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Wiley |
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