Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma

Detalhes bibliográficos
Autor(a) principal: Honorato, Jessica R.
Data de Publicação: 2020
Outros Autores: Hauser-Davis, Rachel A., Saggioro, Enrico M., Correia, Fábio V., Sales Junior, Sidney F., Soares, Lorena O. S., Lima, Leandro da R., Moura Neto, Vivaldo, Lopes, Giselle P de F, Spohr, Tania C. L. de S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43025
Resumo: Acesso aberto em 27/08;/2020 - https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29274
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spelling Honorato, Jessica R.Hauser-Davis, Rachel A.Saggioro, Enrico M.Correia, Fábio V.Sales Junior, Sidney F.Soares, Lorena O. S.Lima, Leandro da R.Moura Neto, VivaldoLopes, Giselle P de FSpohr, Tania C. L. de S.2020-08-27T12:49:06Z2020-08-27T12:49:06Z2020HONORATO, Jessica R. et al. Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma. Journal of Cellular Physiology, v. 235, p. 3798-3814, 2020.0021-9541https://www.arca.fiocruz.br/handle/icict/4302510.1002/jcp.292741097-4652Acesso aberto em 27/08;/2020 - https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29274Secretaria de Estado de Saúde. Instituto Estadual do Cérebro Paulo Niemeyer. Laboratório de Biomedicina do Cérebro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Programa de Pós‐Graduação em Anatomia Patológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Saneamento e Saúde Ambiental. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Departamento de Ciências Naturais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Programa de Pós‐Graduação em Saúde Pública e Meio Ambiente. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Departamento de Ciências Naturais. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Departamento de Ciências Naturais. Rio de Janeiro, RJ, Brasil.Secretaria de Estado de Saúde. Instituto Estadual do Cérebro Paulo Niemeyer. Laboratório de Biomedicina do Cérebro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Programa de Pós‐Graduação em Anatomia Patológica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Programa de Pós‐Graduação em Anatomia Patológica. Rio de Janeiro, RJ, Brasil / Instituto de Estudos do Mar Almirante Paulo Moreira. Departamento de Biotecnologia Marinha. / Instituto Nacional de Câncer. Coordenação de Pesquisa. Rio de Janeiro, RJ, Brasil.Secretaria de Estado de Saúde. Instituto Estadual do Cérebro Paulo Niemeyer. Laboratório de Biomedicina do Cérebro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Programa de Pós‐Graduação em Anatomia Patológica. Rio de Janeiro, RJ, Brasil.The first-line chemotherapy treatment for Glioblastoma (GBM) - the most aggressive and frequent brain tumor - is temozolomide (TMZ). The Sonic hedgehog (SHH) pathway is involved with GBM tumorigenesis and TMZ chemoresistance. The role of SHH pathway inhibition in the potentiation of TMZ's effects using T98G, U251, and GBM11 cell lines is investigated herein. The combination of GANT-61 and TMZ over 72 hr suggested a synergistic effect. All TMZ-resistant cell lines displayed a significant decrease in cell viability, increased DNA fragmentation and loss of membrane integrity. For T98G cells, G2 /M arrest was observed, while U251 cells presented a significant increase in reactive oxygen species production and catalase activity. All the cell lines presented acidic vesicles formation correlated to Beclin-1 overexpression. The combined treatment also enhanced GLI1 expression, indicating the presence of select resistant cells. The selective inhibition of the SHH pathway potentiated the cytotoxic effect of TMZ, thus becoming a promising in vitro strategy for GBM treatment.engWileyGlioblastomaGANT‐61Ouriço sônicoTemozolomidaGANT‐61GlioblastomaSonic hedgehogTemozolomideRole of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastomainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma
title Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma
spellingShingle Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma
Honorato, Jessica R.
Glioblastoma
GANT‐61
Ouriço sônico
Temozolomida
GANT‐61
Glioblastoma
Sonic hedgehog
Temozolomide
title_short Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma
title_full Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma
title_fullStr Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma
title_full_unstemmed Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma
title_sort Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma
author Honorato, Jessica R.
author_facet Honorato, Jessica R.
Hauser-Davis, Rachel A.
Saggioro, Enrico M.
Correia, Fábio V.
Sales Junior, Sidney F.
Soares, Lorena O. S.
Lima, Leandro da R.
Moura Neto, Vivaldo
Lopes, Giselle P de F
Spohr, Tania C. L. de S.
author_role author
author2 Hauser-Davis, Rachel A.
Saggioro, Enrico M.
Correia, Fábio V.
Sales Junior, Sidney F.
Soares, Lorena O. S.
Lima, Leandro da R.
Moura Neto, Vivaldo
Lopes, Giselle P de F
Spohr, Tania C. L. de S.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Honorato, Jessica R.
Hauser-Davis, Rachel A.
Saggioro, Enrico M.
Correia, Fábio V.
Sales Junior, Sidney F.
Soares, Lorena O. S.
Lima, Leandro da R.
Moura Neto, Vivaldo
Lopes, Giselle P de F
Spohr, Tania C. L. de S.
dc.subject.other.pt_BR.fl_str_mv Glioblastoma
GANT‐61
Ouriço sônico
Temozolomida
topic Glioblastoma
GANT‐61
Ouriço sônico
Temozolomida
GANT‐61
Glioblastoma
Sonic hedgehog
Temozolomide
dc.subject.en.pt_BR.fl_str_mv GANT‐61
Glioblastoma
Sonic hedgehog
Temozolomide
description Acesso aberto em 27/08;/2020 - https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29274
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-08-27T12:49:06Z
dc.date.available.fl_str_mv 2020-08-27T12:49:06Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HONORATO, Jessica R. et al. Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma. Journal of Cellular Physiology, v. 235, p. 3798-3814, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43025
dc.identifier.issn.pt_BR.fl_str_mv 0021-9541
dc.identifier.doi.none.fl_str_mv 10.1002/jcp.29274
dc.identifier.eissn.none.fl_str_mv 1097-4652
identifier_str_mv HONORATO, Jessica R. et al. Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma. Journal of Cellular Physiology, v. 235, p. 3798-3814, 2020.
0021-9541
10.1002/jcp.29274
1097-4652
url https://www.arca.fiocruz.br/handle/icict/43025
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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