Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes

Detalhes bibliográficos
Autor(a) principal: Pérez-Díaz, Leticia
Data de Publicação: 2013
Outros Autores: Pastro, Lucía, Smircich, Pablo, Dallagiovanna, Bruno, Garat, Beatriz
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15827
Resumo: Facultad de Ciencias. Laboratorio de Interacciones Moleculares. Montevideo, Uruguay.
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spelling Pérez-Díaz, LeticiaPastro, LucíaSmircich, PabloDallagiovanna, BrunoGarat, Beatriz2016-09-16T18:17:03Z2016-09-16T18:17:03Z2013PÉREZ-DIAZ, Letícia et al. Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes. Biochem. Biophys. Res. Commun. v. 28, n. 436, p. 295-299, 2013.0006-291Xhttps://www.arca.fiocruz.br/handle/icict/1582710.1016/j.bbrc.2013.05.096porW. BaumeisterEvidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFacultad de Ciencias. Laboratorio de Interacciones Moleculares. Montevideo, Uruguay.Facultad de Ciencias. Laboratorio de Interacciones Moleculares. Montevideo, Uruguay.Facultad de Ciencias. Laboratorio de Interacciones Moleculares. Montevideo, Uruguay.Facultad de Ciencias. Laboratorio de Interacciones Moleculares. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Because of their relevant role in the post-transcriptional regulation of the expression of a multitude of genes, RNA-binding proteins (RBPs) need to be accurately regulated in response to environmental signals in terms of quantity, functionality and localization. Transcriptional, post-transcriptional and post-translational steps have all been involved in this tight control. We have previously identified a Trypanosoma cruzi RBP, named TcRBP19, which can barely be detected at the replicative intracellular amastigote stage of the mammalian host. Even though protein coding genes are typically transcribed constitutively in trypanosomes, TcRBP19 protein is undetectable at the epimastigote stage. Here, we show that this protein expression pattern follows the steady-state of its mRNA. Using a T. cruzi reporter gene approach, we could establish a role for the 3' UTR of the tcrbp19 mRNA in transcript down-regulation at the epimastigote stage. In addition, the binding of the TcRBP19 protein to its encoding mRNA was revealed by in vitro pull down followed by qRT-PCR and confirmed by CLIP assays. Furthermore, we found that forced over-expression of TcRBP19 in T. cruzi epimastigotes decreased the stability of the endogenous tcrbp19 mRNA. These results support a negative feedback control of TcRBP19 to help maintain its very low concentration of TcRBP19 in the epimastigote stage. To our knowledge, this is the first RBP reported in trypanosomatids capable of negatively regulating its own mRNA. The mechanism revealed here adds to our limited but growing number of examples of negative mRNA autoregulation in the control of gene expression.Trypanosoma cruzi3′UTRmRNA abundanceRRM proteinAutoregulationRNA MensageiroProteínas com Motivo de Reconhecimento de RNAHomeostaseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15827/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALEvidence for a negative feedback control.pdfEvidence for a negative feedback control.pdfapplication/pdf635554https://www.arca.fiocruz.br/bitstream/icict/15827/2/Evidence%20for%20a%20negative%20feedback%20control.pdff844a9e150b225352c1e155707018c7dMD52TEXTEvidence for a negative feedback control.pdf.txtEvidence for a negative feedback control.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes
title Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes
spellingShingle Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes
Pérez-Díaz, Leticia
Trypanosoma cruzi
3′UTR
mRNA abundance
RRM protein
Autoregulation
RNA Mensageiro
Proteínas com Motivo de Reconhecimento de RNA
Homeostase
title_short Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes
title_full Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes
title_fullStr Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes
title_full_unstemmed Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes
title_sort Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes
author Pérez-Díaz, Leticia
author_facet Pérez-Díaz, Leticia
Pastro, Lucía
Smircich, Pablo
Dallagiovanna, Bruno
Garat, Beatriz
author_role author
author2 Pastro, Lucía
Smircich, Pablo
Dallagiovanna, Bruno
Garat, Beatriz
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pérez-Díaz, Leticia
Pastro, Lucía
Smircich, Pablo
Dallagiovanna, Bruno
Garat, Beatriz
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
3′UTR
mRNA abundance
RRM protein
Autoregulation
topic Trypanosoma cruzi
3′UTR
mRNA abundance
RRM protein
Autoregulation
RNA Mensageiro
Proteínas com Motivo de Reconhecimento de RNA
Homeostase
dc.subject.decs.pt_BR.fl_str_mv RNA Mensageiro
Proteínas com Motivo de Reconhecimento de RNA
Homeostase
description Facultad de Ciencias. Laboratorio de Interacciones Moleculares. Montevideo, Uruguay.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-09-16T18:17:03Z
dc.date.available.fl_str_mv 2016-09-16T18:17:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PÉREZ-DIAZ, Letícia et al. Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes. Biochem. Biophys. Res. Commun. v. 28, n. 436, p. 295-299, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15827
dc.identifier.issn.pt_BR.fl_str_mv 0006-291X
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.bbrc.2013.05.096
identifier_str_mv PÉREZ-DIAZ, Letícia et al. Evidence for a negative feedback control mediated by the 30 untranslated region assuring the low expression level of the RNA binding protein TcRBP19 in T. cruzi epimastigotes. Biochem. Biophys. Res. Commun. v. 28, n. 436, p. 295-299, 2013.
0006-291X
10.1016/j.bbrc.2013.05.096
url https://www.arca.fiocruz.br/handle/icict/15827
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv W. Baumeister
publisher.none.fl_str_mv W. Baumeister
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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