Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: Description of an optimized immunohistochemistry method

Detalhes bibliográficos
Autor(a) principal: Santos, Isabele Barbieri dos
Data de Publicação: 2015
Outros Autores: Tortelly, Rogerio, Quintella, Leonardo Pereira, Madeira, Maria de Fátima, Miranda, Luisa Helena Monteiro de, Figueiredo, Fabiano Borges, Oliveira, Raquel de Vasconcellos Carvalhaes de, Schubach, Tânia Maria Pacheco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35092
Resumo: 2020-08-27
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spelling Santos, Isabele Barbieri dosTortelly, RogerioQuintella, Leonardo PereiraMadeira, Maria de FátimaMiranda, Luisa Helena Monteiro deFigueiredo, Fabiano BorgesOliveira, Raquel de Vasconcellos Carvalhaes deSchubach, Tânia Maria Pacheco2019-08-27T13:34:55Z2019-08-27T13:34:55Z2015SANTOS, Isabele Barbieri dos et al. Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: description of an optimized immunohistochemistry method. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 109, p. 469-476, 2015.0035-9203https://www.arca.fiocruz.br/handle/icict/3509210.1093/trstmh/trv0341878-3503engOxford University PressHigher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: Description of an optimized immunohistochemistry methodinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-08-27Fundação Oswaldo Cruz. DIREB. Programa de Epidemiologia e Vigilância em Saúde. Brasília, DF, Brasil.Universidade Federal Fluminense. Faculdade de Veterinária. Serviço de Anatomia Patológica Doutor Jefferson Andrade dos Santos. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Anatomia Patológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Dermatozoonoses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Dermatozoonoses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Epidemiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Dermatozoonoses. Rio de Janeiro, RJ, Brasil.Background: The in situ detection of parasite antigens in tissue sections by immunohistochemistry (IHC) is a diagnostic alternative for human American tegumentary leishmaniasis (ATL), but has not been used for the diagnosis of cutaneous lesions in dogs with ATL. This study describes the results of IHC for the detection of amastigote forms and other Leishmania sp. antigen-positive cells and compares the results of IHC, histopathology and cytopathology for the diagnosis of canine ATL. In addition, possible cross-reactivity with sporotrichosis is analyzed. Methods: Forty paraffin-embedded biopsies and 40 smears of cutaneous lesions from dogs with ATL, confirmed by isolation and characterization of Leishmania (Viannia) braziliensis, and 40 paraffin-embedded biopsies of cutaneous lesions from dogs with sporotrichosis, confirmed by isolation of Sporothrix schenckii in culture (control group), were studied. Results: Immunohistochemistry was more sensitive in detecting amastigote forms than cytopathology and histopathology, with a positivity rate of 70% (n¼28) versus 37.5% and 22.5% for histopathology and cytopathology, respectively. Cytoplasmic staining of mononuclear and endothelial cells was detected by IHC, which was highly specific since no cytoplasmic staining of these cells or staining of fungal structures was observed in sporotrichosis fragments. Conclusions: In view of the higher sensitivity of IHC in detecting Leishmania sp. antigen and patterns of positivity for Leishmania sp. antigen compared to histopathology or cytopathology and the absence of cross-reactions with sporotrichosis, we recommend this technique for the diagnosis of canine tegumentary leishmaniasis.American tegumentary leishmaniasisBrazilDogsImmunohistochemistryinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/35092/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Santos_Isabele_etal_INI_2015.pdfve_Santos_Isabele_etal_INI_2015.pdfapplication/pdf861224https://www.arca.fiocruz.br/bitstream/icict/35092/2/ve_Santos_Isabele_etal_INI_2015.pdf11d22dbf8733a28b080c63ca9a58c5c4MD52TEXTve_Santos_Isabele_etal_INI_2015.pdf.txtve_Santos_Isabele_etal_INI_2015.pdf.txtExtracted texttext/plain41056https://www.arca.fiocruz.br/bitstream/icict/35092/3/ve_Santos_Isabele_etal_INI_2015.pdf.txt303556a72c00434d557fa5de8f197829MD53icict/350922021-03-24 16:32:35.788oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: Description of an optimized immunohistochemistry method
title Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: Description of an optimized immunohistochemistry method
spellingShingle Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: Description of an optimized immunohistochemistry method
Santos, Isabele Barbieri dos
American tegumentary leishmaniasis
Brazil
Dogs
Immunohistochemistry
title_short Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: Description of an optimized immunohistochemistry method
title_full Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: Description of an optimized immunohistochemistry method
title_fullStr Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: Description of an optimized immunohistochemistry method
title_full_unstemmed Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: Description of an optimized immunohistochemistry method
title_sort Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: Description of an optimized immunohistochemistry method
author Santos, Isabele Barbieri dos
author_facet Santos, Isabele Barbieri dos
Tortelly, Rogerio
Quintella, Leonardo Pereira
Madeira, Maria de Fátima
Miranda, Luisa Helena Monteiro de
Figueiredo, Fabiano Borges
Oliveira, Raquel de Vasconcellos Carvalhaes de
Schubach, Tânia Maria Pacheco
author_role author
author2 Tortelly, Rogerio
Quintella, Leonardo Pereira
Madeira, Maria de Fátima
Miranda, Luisa Helena Monteiro de
Figueiredo, Fabiano Borges
Oliveira, Raquel de Vasconcellos Carvalhaes de
Schubach, Tânia Maria Pacheco
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Isabele Barbieri dos
Tortelly, Rogerio
Quintella, Leonardo Pereira
Madeira, Maria de Fátima
Miranda, Luisa Helena Monteiro de
Figueiredo, Fabiano Borges
Oliveira, Raquel de Vasconcellos Carvalhaes de
Schubach, Tânia Maria Pacheco
dc.subject.en.pt_BR.fl_str_mv American tegumentary leishmaniasis
Brazil
Dogs
Immunohistochemistry
topic American tegumentary leishmaniasis
Brazil
Dogs
Immunohistochemistry
description 2020-08-27
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2019-08-27T13:34:55Z
dc.date.available.fl_str_mv 2019-08-27T13:34:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Isabele Barbieri dos et al. Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: description of an optimized immunohistochemistry method. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 109, p. 469-476, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35092
dc.identifier.issn.pt_BR.fl_str_mv 0035-9203
dc.identifier.doi.none.fl_str_mv 10.1093/trstmh/trv034
dc.identifier.eissn.none.fl_str_mv 1878-3503
identifier_str_mv SANTOS, Isabele Barbieri dos et al. Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: description of an optimized immunohistochemistry method. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 109, p. 469-476, 2015.
0035-9203
10.1093/trstmh/trv034
1878-3503
url https://www.arca.fiocruz.br/handle/icict/35092
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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