Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Salomão, Kelly
Data de Publicação: 2011
Outros Autores: Souza, Eniuce M. de, Pons, Andrea Henriques, Barbosa, Helene S., Castro, Solange L. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20919
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
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spelling Salomão, KellySouza, Eniuce M. dePons, Andrea HenriquesBarbosa, Helene S.Castro, Solange L. de2017-09-05T15:16:52Z2017-09-05T15:16:52Z2011SALOMÂO, Kelly; et al. Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi. Evidence-Based Complementary and Alternative Medicine, Volume 2011, Article ID 185918, 11p, 2011.1741-427Xhttps://www.arca.fiocruz.br/handle/icict/2091910.1093/ecam/nep0141741-4288engHindawi Publishing CorporationTrypanosoma cruziIn vitroIn vivoPrópoleBrasilTrypanosoma cruziIn vitroIn vivoBrazilian green propolisBrazilBrazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.The composition of a Brazilian green propolis ethanolic extract (Et-Bra) and its effect on Trypanosoma cruzi trypomastigotes and other pathogenic microorganisms have already been reported. Here, we further investigated Et-Bra targets in T. cruzi and its effect on experimental infection of mice. The IC(50)/4 days for inhibition of amastigote proliferation was 8.5 ± 1.8 μg mL(-1), with no damage to the host cells. In epimastigotes Et-Bra induced alterations in reservosomes, Golgi complex and mitochondrion. These effects were confirmed by flow cytometry analysis. In trypomastigotes, Et-Bra led to the loss of plasma membrane integrity. The in vitro studies indicate that Et-Bra interferes in the functionality of the plasma membrane in trypomastigotes and of reservosomes and mitochondrion in epimastigotes. Acutely infected mice were treated orally with Et-Bra and the parasitemia, mortality and GPT, GOT, CK and urea levels were monitored. The extract (25-300 mg kg(-1) body weight/day for 10 days) reduced the parasitemia, although not at significant levels; increased the survival of the animals and did not induce any hepatic, muscular lesion or renal toxicity. Since Et-Bra was not toxic to the animals, it could be assayed in combination with other drugs. Et-Bra could be a potential metacyclogenesis blocker, considering its effect on reservosomes, which are an important energy source during parasite differentiation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20919/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALhelene2_barbosa_etal_IOC_2009.pdfhelene2_barbosa_etal_IOC_2009.pdfapplication/pdf4890491https://www.arca.fiocruz.br/bitstream/icict/20919/2/helene2_barbosa_etal_IOC_2009.pdf5c1e343cc63060996ab4f3c83d82a78cMD52TEXThelene2_barbosa_etal_IOC_2009.pdf.txthelene2_barbosa_etal_IOC_2009.pdf.txtExtracted texttext/plain43277https://www.arca.fiocruz.br/bitstream/icict/20919/3/helene2_barbosa_etal_IOC_2009.pdf.txt04c48986e0d1e853bf2105878e95296fMD53icict/209192018-08-15 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dc.title.pt_BR.fl_str_mv Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi
title Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi
spellingShingle Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi
Salomão, Kelly
Trypanosoma cruzi
In vitro
In vivo
Própole
Brasil
Trypanosoma cruzi
In vitro
In vivo
Brazilian green propolis
Brazil
title_short Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi
title_full Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi
title_fullStr Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi
title_full_unstemmed Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi
title_sort Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi
author Salomão, Kelly
author_facet Salomão, Kelly
Souza, Eniuce M. de
Pons, Andrea Henriques
Barbosa, Helene S.
Castro, Solange L. de
author_role author
author2 Souza, Eniuce M. de
Pons, Andrea Henriques
Barbosa, Helene S.
Castro, Solange L. de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Salomão, Kelly
Souza, Eniuce M. de
Pons, Andrea Henriques
Barbosa, Helene S.
Castro, Solange L. de
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
In vitro
In vivo
Própole
Brasil
topic Trypanosoma cruzi
In vitro
In vivo
Própole
Brasil
Trypanosoma cruzi
In vitro
In vivo
Brazilian green propolis
Brazil
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
In vitro
In vivo
Brazilian green propolis
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2017-09-05T15:16:52Z
dc.date.available.fl_str_mv 2017-09-05T15:16:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SALOMÂO, Kelly; et al. Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi. Evidence-Based Complementary and Alternative Medicine, Volume 2011, Article ID 185918, 11p, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20919
dc.identifier.issn.pt_BR.fl_str_mv 1741-427X
dc.identifier.doi.none.fl_str_mv 10.1093/ecam/nep014
dc.identifier.eissn.none.fl_str_mv 1741-4288
identifier_str_mv SALOMÂO, Kelly; et al. Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi. Evidence-Based Complementary and Alternative Medicine, Volume 2011, Article ID 185918, 11p, 2011.
1741-427X
10.1093/ecam/nep014
1741-4288
url https://www.arca.fiocruz.br/handle/icict/20919
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/20919/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/20919/2/helene2_barbosa_etal_IOC_2009.pdf
https://www.arca.fiocruz.br/bitstream/icict/20919/3/helene2_barbosa_etal_IOC_2009.pdf.txt
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