Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet

Detalhes bibliográficos
Autor(a) principal: Neves, Renata Heisler
Data de Publicação: 2006
Outros Autores: Alencar, Alba Cristina Miranda de Barros, Aguila, Marcia Barbosa, Lacerda, Carlos Alberto Mandarim de, Silva, José Roberto Machado, Gomes, Delir Corrêa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39470
Resumo: Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Roberto Lascasas Porto. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.
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spelling Neves, Renata HeislerAlencar, Alba Cristina Miranda de BarrosAguila, Marcia BarbosaLacerda, Carlos Alberto Mandarim deSilva, José Roberto MachadoGomes, Delir Corrêa2020-01-23T15:33:18Z2020-01-23T15:33:18Z2006NEVES, Renata Heisler et al. Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet. International Journal of Morphology, v. 24, n. 4, p. 625-632, 2006.0717-9367https://www.arca.fiocruz.br/handle/icict/3947010.4067/S0717-950220060005000180717-9502engDesconhecidoHipercolesterolemiaModelo experimentalRatosColesterolDietaFígadoHypercholesterolemiaExperimental modelMiceCholesterolDietLiverSomatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat DietAlteraciones Somáticas Bioquímicas y hepáticas en un Tipo de Ratas Alimentadas Crónicamente con Dieta Alta en Grasasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Roberto Lascasas Porto. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Roberto Lascasas Porto. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia. Laboratório de Morfometria e Morfologia Cardiovascular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Centro Biomédico. Instituto de Biologia. Laboratório de Morfometria e Morfologia Cardiovascular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Roberto Lascasas Porto. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.This study evaluated whether a high fat diet (HFC group) induces overweight, hepatic steatosis and plasma lipoproteins level alteration compared to standard chow diet (SC group). Female mice were submitted to each diet over 6 months. Body mass and food intake were evaluated weekly throughout the experiment. Total cholesterol, TG, LDL-c, HDL-c and VLDL-c were analyzed. Mice were sacrificed to remove liver, spleen, heart and intestine. The volume of the organs was determined according to the submersion method. Fixed livers were embedded in paraffin and stained with hematoxylin and eosin and Masson’s trichrome. The analysis used a video microscope system and a test-system with 42 test-points. The volume density was estimated for hepatocytes, steatosis and sinusoids. Animals fed HFC had smaller chow intake than SC group. HFC group presented body mass greater than SC. Animals fed HFC showed heavier liver and spleen and lighter intestine than SC (p<0.05), heart mass was not significant between groups. Plasma lipoproteins differed between groups (p<0.05) except VLDL-c and TG fractions. The liver structure was without major alteration in SC group however, HFC mice group showed different degrees of fatty degeneration with micro- and macrovesicular steatosis dispersed in all liver with typical peri-cellular/peri-sinusoidal fibrosis. The quantitative study showed significant (p<0.05) volume density reduction for hepatocytes and sinusoids. In conclusion, our results clearly show that hepatic steatosis can be induced in mouse by such a fat-rich diet without any toxin ingestion, alimentary deficiency and genes depletion.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39470/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJoseRobertoM_SIlva_etal_IOC_2006.pdfJoseRobertoM_SIlva_etal_IOC_2006.pdfapplication/pdf1507551https://www.arca.fiocruz.br/bitstream/icict/39470/2/JoseRobertoM_SIlva_etal_IOC_2006.pdf432b8f82d3c6ece2da59812435aa2197MD52TEXTJoseRobertoM_SIlva_etal_IOC_2006.pdf.txtJoseRobertoM_SIlva_etal_IOC_2006.pdf.txtExtracted texttext/plain32716https://www.arca.fiocruz.br/bitstream/icict/39470/3/JoseRobertoM_SIlva_etal_IOC_2006.pdf.txt5cccc3ba3309eaad87712984cc3caf3eMD53icict/394702020-01-30 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dc.title.pt_BR.fl_str_mv Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
dc.title.alternative.pt_BR.fl_str_mv Alteraciones Somáticas Bioquímicas y hepáticas en un Tipo de Ratas Alimentadas Crónicamente con Dieta Alta en Grasas
title Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
spellingShingle Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
Neves, Renata Heisler
Hipercolesterolemia
Modelo experimental
Ratos
Colesterol
Dieta
Fígado
Hypercholesterolemia
Experimental model
Mice
Cholesterol
Diet
Liver
title_short Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
title_full Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
title_fullStr Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
title_full_unstemmed Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
title_sort Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
author Neves, Renata Heisler
author_facet Neves, Renata Heisler
Alencar, Alba Cristina Miranda de Barros
Aguila, Marcia Barbosa
Lacerda, Carlos Alberto Mandarim de
Silva, José Roberto Machado
Gomes, Delir Corrêa
author_role author
author2 Alencar, Alba Cristina Miranda de Barros
Aguila, Marcia Barbosa
Lacerda, Carlos Alberto Mandarim de
Silva, José Roberto Machado
Gomes, Delir Corrêa
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Neves, Renata Heisler
Alencar, Alba Cristina Miranda de Barros
Aguila, Marcia Barbosa
Lacerda, Carlos Alberto Mandarim de
Silva, José Roberto Machado
Gomes, Delir Corrêa
dc.subject.other.pt_BR.fl_str_mv Hipercolesterolemia
Modelo experimental
Ratos
Colesterol
Dieta
Fígado
topic Hipercolesterolemia
Modelo experimental
Ratos
Colesterol
Dieta
Fígado
Hypercholesterolemia
Experimental model
Mice
Cholesterol
Diet
Liver
dc.subject.en.pt_BR.fl_str_mv Hypercholesterolemia
Experimental model
Mice
Cholesterol
Diet
Liver
description Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Roberto Lascasas Porto. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2020-01-23T15:33:18Z
dc.date.available.fl_str_mv 2020-01-23T15:33:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NEVES, Renata Heisler et al. Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet. International Journal of Morphology, v. 24, n. 4, p. 625-632, 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39470
dc.identifier.issn.pt_BR.fl_str_mv 0717-9367
dc.identifier.doi.none.fl_str_mv 10.4067/S0717-95022006000500018
dc.identifier.eissn.none.fl_str_mv 0717-9502
identifier_str_mv NEVES, Renata Heisler et al. Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet. International Journal of Morphology, v. 24, n. 4, p. 625-632, 2006.
0717-9367
10.4067/S0717-95022006000500018
0717-9502
url https://www.arca.fiocruz.br/handle/icict/39470
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Desconhecido
publisher.none.fl_str_mv Desconhecido
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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