Trypsin-Like Serine Proteases in Lutzomyia longipalpis –Expression, Activity and Possible Modulation by Leishmania infantum chagasi

Detalhes bibliográficos
Autor(a) principal: Telleria, Erich Loza
Data de Publicação: 2010
Outros Autores: Araujo, Adriana Pereira Oliveira de, Secundino, Nágila Francinete Costa, Levy, Claudia Masini D’Avila, Traub Cseko, Yara Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/5894
Resumo: CNPq, FAPERJ and FIOCRUZ
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spelling Telleria, Erich LozaAraujo, Adriana Pereira Oliveira deSecundino, Nágila Francinete CostaLevy, Claudia Masini D’AvilaTraub Cseko, Yara Maria2012-11-28T13:36:24Z2012-11-28T13:36:24Z2010TELLERIA, Erich Loza. Trypsin-Like Serine Proteases in Lutzomyia longipalpis – Expression, Activity and Possible Modulation by Leishmania infantum chagasi. PLoS ONE 5(5): e10697, 20101932-6203https://www.arca.fiocruz.br/handle/icict/5894CNPq, FAPERJ and FIOCRUZFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brazil,Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Entomologia Medica. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Biologia Molecular e Doenças Endemicas. Rio de Janeiro, RJ, BrazilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, BrazilBackground: Midgut enzymatic activity is one of the obstacles that Leishmania must surpass to succeed in establishing infection. Trypsins are abundant digestive enzymes in most insects. We have previously described two trypsin cDNAs of L. longipalpis: one (Lltryp1) with a bloodmeal induced transcription pattern, the other (Lltryp2) with a constitutive transcription pattern. We have now characterized the expression and activity of trypsin-like proteases of Lutzomyia longipalpis, the main vector of visceral leishmaniasis in Brazil. Methodology and Principal Findings: In order to study trypsin expression profiles we produced antibodies against peptides specific for Lltryp1 and Lltryp2. The anti-Lltryp1-peptide antibody revealed a band of 28 kDa between 6 and 48 hours. The anti-Lltryp2 peptide antibody did not evidence any band. When proteinaceous substrates (gelatin, hemoglobin, casein or albumin) were co-polymerized in polyacrylamide gels, insect midguts obtained at 12 hours after feeding showed a unique proteolytic pattern for each substrate. All activity bands were strongly inhibited by TLCK, benzamidine and 4-amino-benzamidine, indicating that they are trypsin-like proteases. The trypsin-like activity was also measured in vitro at different time points after ingestion of blood or blood containing Leishmania infantum chagasi, using the chromogenic substrate BArNA. L. longipalpis females fed on blood infected with L. i. chagasi had lower levels of trypsin activity after 12 and 48 hours than non-infected insects, suggesting that the parasite may have a role in this modulation. Conclusions and Significance: Trypsins are important and abundant digestive enzymes in L. longipalpis. Protein production and enzymatic activity followed previously identified gene expression of a blood modulated trypsin gene. A decrease of enzymatic activity upon the parasite infection, previously detected mostly in Old World vectors, was detected for the first time in the natural vector-parasite pair L. longipalpis-L. i. chagasi.engTrypsin-Like Serine Proteases in Lutzomyia longipalpis –Expression, Activity and Possible Modulation by Leishmania infantum chagasiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleleishmaniasisSynthetic trypsin peptidesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL124.2010.pdf124.2010.pdfapplication/pdf1176852https://www.arca.fiocruz.br/bitstream/icict/5894/1/124.2010.pdfab1571c2958f1ed490e2b4b972af42bfMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5894/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXT124.2010.pdf.txt124.2010.pdf.txtExtracted texttext/plain37513https://www.arca.fiocruz.br/bitstream/icict/5894/5/124.2010.pdf.txt6db5056c0af73ac5d194d47f5a61961dMD55THUMBNAIL124.2010.pdf.jpg124.2010.pdf.jpgGenerated Thumbnailimage/jpeg2581https://www.arca.fiocruz.br/bitstream/icict/5894/4/124.2010.pdf.jpg8f6252ea60bdd51ce2d312174fb771c2MD54icict/58942018-05-18 08:22:41.4oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-05-18T11:22:41Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Trypsin-Like Serine Proteases in Lutzomyia longipalpis –Expression, Activity and Possible Modulation by Leishmania infantum chagasi
title Trypsin-Like Serine Proteases in Lutzomyia longipalpis –Expression, Activity and Possible Modulation by Leishmania infantum chagasi
spellingShingle Trypsin-Like Serine Proteases in Lutzomyia longipalpis –Expression, Activity and Possible Modulation by Leishmania infantum chagasi
Telleria, Erich Loza
leishmaniasis
Synthetic trypsin peptides
title_short Trypsin-Like Serine Proteases in Lutzomyia longipalpis –Expression, Activity and Possible Modulation by Leishmania infantum chagasi
title_full Trypsin-Like Serine Proteases in Lutzomyia longipalpis –Expression, Activity and Possible Modulation by Leishmania infantum chagasi
title_fullStr Trypsin-Like Serine Proteases in Lutzomyia longipalpis –Expression, Activity and Possible Modulation by Leishmania infantum chagasi
title_full_unstemmed Trypsin-Like Serine Proteases in Lutzomyia longipalpis –Expression, Activity and Possible Modulation by Leishmania infantum chagasi
title_sort Trypsin-Like Serine Proteases in Lutzomyia longipalpis –Expression, Activity and Possible Modulation by Leishmania infantum chagasi
author Telleria, Erich Loza
author_facet Telleria, Erich Loza
Araujo, Adriana Pereira Oliveira de
Secundino, Nágila Francinete Costa
Levy, Claudia Masini D’Avila
Traub Cseko, Yara Maria
author_role author
author2 Araujo, Adriana Pereira Oliveira de
Secundino, Nágila Francinete Costa
Levy, Claudia Masini D’Avila
Traub Cseko, Yara Maria
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Telleria, Erich Loza
Araujo, Adriana Pereira Oliveira de
Secundino, Nágila Francinete Costa
Levy, Claudia Masini D’Avila
Traub Cseko, Yara Maria
dc.subject.en.pt_BR.fl_str_mv leishmaniasis
Synthetic trypsin peptides
topic leishmaniasis
Synthetic trypsin peptides
description CNPq, FAPERJ and FIOCRUZ
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2012-11-28T13:36:24Z
dc.date.available.fl_str_mv 2012-11-28T13:36:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv TELLERIA, Erich Loza. Trypsin-Like Serine Proteases in Lutzomyia longipalpis – Expression, Activity and Possible Modulation by Leishmania infantum chagasi. PLoS ONE 5(5): e10697, 2010
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5894
dc.identifier.issn.none.fl_str_mv 1932-6203
identifier_str_mv TELLERIA, Erich Loza. Trypsin-Like Serine Proteases in Lutzomyia longipalpis – Expression, Activity and Possible Modulation by Leishmania infantum chagasi. PLoS ONE 5(5): e10697, 2010
1932-6203
url https://www.arca.fiocruz.br/handle/icict/5894
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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