Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44390 |
Resumo: | Daniella Pereira - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento do vinculo com a Fiocruz Brasília. |
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Genteluci, Gabrielle LimeiraGomes, Daniela Betzler CardosoPereira, Daniella Cristina RodriguesNeves, Marta de CamposSouza, Maria José deRangel, KaryneVillas Bôas, Maria Helena Simões2020-11-12T20:57:37Z2020-11-12T20:57:37Z2020GENTELUCI, Gabrielle Limeira et al. Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells. Brazilian Journal of Microbiology, São Paulo, v. 51, p. 657–664, mar. 2020.1517-8382https://www.arca.fiocruz.br/handle/icict/4439010.1007/s42770-020-00252-xDaniella Pereira - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento do vinculo com a Fiocruz Brasília.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Programa de Pós-Graduação em Vigilância em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Programa de Pós-Graduação em Vigilância em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Federal Hospital of State Servers. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Acinetobacter baumannii has been associated with antimicrobial resistance and ability to form biofilms. Furthermore, its adherence to host cells is an important factor to the colonization process. Therefore, this study intended to identify some virulence factors that can explain the success of A. baumannii in causing nosocomial infections. We studied 92 A. baumannii isolates collected from hospitals in Rio de Janeiro, Brazil. Isolates were identified and the susceptibility to antimicrobials was determined. Oxacilinase type β-lactamase encoding genes were amplified by polymerase chain reaction, and genetic diversity was investigated by pulsed-field gel electrophoresis (PFGE). In addition, biofilm formation on polystyrene plates using crystal violet staining was quantified, and adherence to human cell lines was evaluated. Eighty-six isolates were multidrug-resistant, of which 93% were carbapenem-resistant. All isolates had the blaOXA-51 gene and 94% had the blaOXA-23 gene, other searched blaOXA genes were not detected. PFGE typing showed two predominant clones, and biofilm production was observed in 79%of isolates. A. baumannii isolates adhered better to HEp-2 cell compared with A-549 cell. Clones A, B, E, and F showed a significantly increased adherence to HEp-2 compared with adherence to A-549 cell. Our findings revealed that A. baumannii isolates had high frequencies of resistance to antimicrobial agents, ability to form biofilm, and capacity to adhere to HEp-2 cells.engSociedade Brasileira de MicrobiologiaAcinetobacter baumanniiDrug Resistance, MicrobialBiofilmsAcinetobacter baumanniiAntimicrobial resistancePCRPFGEBiofilmAdherence to cellAcinetobacter baumanniiResistência Microbiana a MedicamentosBiofilmesMultidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44390/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Gabrielle Limeira Genteluci_etal_2020.pdfve_Gabrielle Limeira 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| dc.title.pt_BR.fl_str_mv |
Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells |
| title |
Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells |
| spellingShingle |
Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells Genteluci, Gabrielle Limeira Acinetobacter baumannii Drug Resistance, Microbial Biofilms Acinetobacter baumannii Antimicrobial resistance PCR PFGE Biofilm Adherence to cell Acinetobacter baumannii Resistência Microbiana a Medicamentos Biofilmes |
| title_short |
Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells |
| title_full |
Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells |
| title_fullStr |
Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells |
| title_full_unstemmed |
Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells |
| title_sort |
Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells |
| author |
Genteluci, Gabrielle Limeira |
| author_facet |
Genteluci, Gabrielle Limeira Gomes, Daniela Betzler Cardoso Pereira, Daniella Cristina Rodrigues Neves, Marta de Campos Souza, Maria José de Rangel, Karyne Villas Bôas, Maria Helena Simões |
| author_role |
author |
| author2 |
Gomes, Daniela Betzler Cardoso Pereira, Daniella Cristina Rodrigues Neves, Marta de Campos Souza, Maria José de Rangel, Karyne Villas Bôas, Maria Helena Simões |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Genteluci, Gabrielle Limeira Gomes, Daniela Betzler Cardoso Pereira, Daniella Cristina Rodrigues Neves, Marta de Campos Souza, Maria José de Rangel, Karyne Villas Bôas, Maria Helena Simões |
| dc.subject.mesh.pt_BR.fl_str_mv |
Acinetobacter baumannii Drug Resistance, Microbial Biofilms |
| topic |
Acinetobacter baumannii Drug Resistance, Microbial Biofilms Acinetobacter baumannii Antimicrobial resistance PCR PFGE Biofilm Adherence to cell Acinetobacter baumannii Resistência Microbiana a Medicamentos Biofilmes |
| dc.subject.en.pt_BR.fl_str_mv |
Acinetobacter baumannii Antimicrobial resistance PCR PFGE Biofilm Adherence to cell |
| dc.subject.decs.pt_BR.fl_str_mv |
Acinetobacter baumannii Resistência Microbiana a Medicamentos Biofilmes |
| description |
Daniella Pereira - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento do vinculo com a Fiocruz Brasília. |
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2020 |
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2020-11-12T20:57:37Z |
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2020-11-12T20:57:37Z |
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2020 |
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info:eu-repo/semantics/article |
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GENTELUCI, Gabrielle Limeira et al. Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells. Brazilian Journal of Microbiology, São Paulo, v. 51, p. 657–664, mar. 2020. |
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https://www.arca.fiocruz.br/handle/icict/44390 |
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1517-8382 |
| dc.identifier.doi.none.fl_str_mv |
10.1007/s42770-020-00252-x |
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GENTELUCI, Gabrielle Limeira et al. Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells. Brazilian Journal of Microbiology, São Paulo, v. 51, p. 657–664, mar. 2020. 1517-8382 10.1007/s42770-020-00252-x |
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Sociedade Brasileira de Microbiologia |
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