Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.

Detalhes bibliográficos
Autor(a) principal: Barocchi, Michele A
Data de Publicação: 2001
Outros Autores: Ko, Albert Icksang, Ferrer, Suzana Ramos, Faria, Marcos Tucunduva de, Reis, Mitermayer Galvão dos, Riley, Lee Woodland
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7567
Resumo: Division of Infectious Diseases and Immunity. School of Public Health. University of California. Berkeley, California
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spelling Barocchi, Michele AKo, Albert IcksangFerrer, Suzana RamosFaria, Marcos Tucunduva deReis, Mitermayer Galvão dosRiley, Lee Woodland2014-05-02T14:12:01Z2014-05-02T14:12:01Z2001BAROCCHI, M. A. et al. Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.0095-1137https://www.arca.fiocruz.br/handle/icict/756710.1128/JCM.39.1.191–195.2001engAmerican Society for MicrobiologyIdentification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDivision of Infectious Diseases and Immunity. School of Public Health. University of California. Berkeley, CaliforniaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Diseases. Weil Medical College of Cornell University. New York, New YorkFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilDivision of Infectious Diseases and Immunity. School of Public Health. University of California. Berkeley, CaliforniaA new repetitive DNA element was identified in an isolate of Leptospira interrogans serovar copenhageni from a patient in Salvador, Brazil. A Sau3A genomic library from this strain was constructed and screened for repetitive DNA elements. An insert of 438 bp (Rep1) from one library clone hybridized to multiple chromosomal DNA fragments resolved electrophoretically after digestion with BamHI, HindIII, and MfeI. A single oligonucleotide primer, designated iRepl, was designed to generate multiple PCR amplicons of various electrophoretic mobilities in a PCR typing method. The method distinguished strains belonging to the eight pathogenic and three saprophytic species of the genus Leptospira. Clinical isolates obtained during urban epidemics between 1996 and 1998 in Salvador, Brazil, were analyzed by this PCR method. Although the iRep1 primer was unable to discriminate strains among L. interrogans serovar copenhageni isolates, it was able to differentiate strains belonging to different species and serogroups of Leptospira identified in Salvador. This PCR-based method may provide a faster and less expensive alternative to serologic tests used in reference laboratories.Leptospira interrogans/classificaçãoLeptospira interrogans/genéticaLeptospira/classificaçãoRepetições de Trinucleotídeos/genéticaDoença de Weil/microbiologiaAnimaisSequência de BasesDNA Bacteriano/análiseDNA Bacteriano/genéticaDosagem de GenesBiblioteca GenômicaHumanosLeptospira/genéticaLeptospira interrogans/isolamento & purificaçãoDados de Sequência MolecularReação em Cadeia da Polimerase/métodosAnálise de Sequência de DNASorotipagemDoença de Weil/epidemiologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBarocchi MA Identification of New....pdfBarocchi MA Identification of New....pdfapplication/pdf2689916https://www.arca.fiocruz.br/bitstream/icict/7567/1/Barocchi%20MA%20Identification%20of%20New....pdfb29266c121b560e1b5c6260b77f5c08fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7567/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBarocchi MA Identification of New....pdf.txtBarocchi MA Identification of New....pdf.txtExtracted texttext/plain31486https://www.arca.fiocruz.br/bitstream/icict/7567/5/Barocchi%20MA%20Identification%20of%20New....pdf.txt1777ddb564ce198b4228fd1cb193a36aMD55THUMBNAILBarocchi MA Identification of New....pdf.jpgBarocchi MA Identification of New....pdf.jpgGenerated Thumbnailimage/jpeg2050https://www.arca.fiocruz.br/bitstream/icict/7567/4/Barocchi%20MA%20Identification%20of%20New....pdf.jpgefbf4a14b085cf16feeb7f1903dce005MD54icict/75672023-03-15 14:33:18.504oai:www.arca.fiocruz.br:icict/7567TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
title Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
spellingShingle Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
Barocchi, Michele A
Leptospira interrogans/classificação
Leptospira interrogans/genética
Leptospira/classificação
Repetições de Trinucleotídeos/genética
Doença de Weil/microbiologia
Animais
Sequência de Bases
DNA Bacteriano/análise
DNA Bacteriano/genética
Dosagem de Genes
Biblioteca Genômica
Humanos
Leptospira/genética
Leptospira interrogans/isolamento & purificação
Dados de Sequência Molecular
Reação em Cadeia da Polimerase/métodos
Análise de Sequência de DNA
Sorotipagem
Doença de Weil/epidemiologia
title_short Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
title_full Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
title_fullStr Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
title_full_unstemmed Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
title_sort Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
author Barocchi, Michele A
author_facet Barocchi, Michele A
Ko, Albert Icksang
Ferrer, Suzana Ramos
Faria, Marcos Tucunduva de
Reis, Mitermayer Galvão dos
Riley, Lee Woodland
author_role author
author2 Ko, Albert Icksang
Ferrer, Suzana Ramos
Faria, Marcos Tucunduva de
Reis, Mitermayer Galvão dos
Riley, Lee Woodland
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Barocchi, Michele A
Ko, Albert Icksang
Ferrer, Suzana Ramos
Faria, Marcos Tucunduva de
Reis, Mitermayer Galvão dos
Riley, Lee Woodland
dc.subject.decs.pt_BR.fl_str_mv Leptospira interrogans/classificação
Leptospira interrogans/genética
Leptospira/classificação
Repetições de Trinucleotídeos/genética
Doença de Weil/microbiologia
Animais
Sequência de Bases
DNA Bacteriano/análise
DNA Bacteriano/genética
Dosagem de Genes
Biblioteca Genômica
Humanos
Leptospira/genética
Leptospira interrogans/isolamento & purificação
Dados de Sequência Molecular
Reação em Cadeia da Polimerase/métodos
Análise de Sequência de DNA
Sorotipagem
Doença de Weil/epidemiologia
topic Leptospira interrogans/classificação
Leptospira interrogans/genética
Leptospira/classificação
Repetições de Trinucleotídeos/genética
Doença de Weil/microbiologia
Animais
Sequência de Bases
DNA Bacteriano/análise
DNA Bacteriano/genética
Dosagem de Genes
Biblioteca Genômica
Humanos
Leptospira/genética
Leptospira interrogans/isolamento & purificação
Dados de Sequência Molecular
Reação em Cadeia da Polimerase/métodos
Análise de Sequência de DNA
Sorotipagem
Doença de Weil/epidemiologia
description Division of Infectious Diseases and Immunity. School of Public Health. University of California. Berkeley, California
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2014-05-02T14:12:01Z
dc.date.available.fl_str_mv 2014-05-02T14:12:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BAROCCHI, M. A. et al. Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7567
dc.identifier.issn.none.fl_str_mv 0095-1137
dc.identifier.doi.none.fl_str_mv 10.1128/JCM.39.1.191–195.2001
identifier_str_mv BAROCCHI, M. A. et al. Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
0095-1137
10.1128/JCM.39.1.191–195.2001
url https://www.arca.fiocruz.br/handle/icict/7567
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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