Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo

Detalhes bibliográficos
Autor(a) principal: Carvalho, Leonardo J. M.
Data de Publicação: 2020
Outros Autores: Tuvshintulga, Bunduurem, Nugraha, Arifin B., Sivakumar, Thillaiampalam Sivakumar, Yokoyama, Naoaki
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45543
Resumo: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan / Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
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spelling Carvalho, Leonardo J. M.Tuvshintulga, BunduuremNugraha, Arifin B.Sivakumar, Thillaiampalam SivakumarYokoyama, Naoaki2021-01-09T18:03:08Z2021-01-09T18:03:08Z2020CARVALHO, Leonardo J. M. et al. Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo. Parasites & Vectors, v. 13, n. 362, p. 1-9, 2020.1756-3305https://www.arca.fiocruz.br/handle/icict/4554310.1186/s13071-020-04235-7engBMCArtesunatoArtesunatoBabesiaArtesunateTafenoquineArtesunatoActivities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan / Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan / Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan / OIE Reference Laboratory for Bovine Babesiosis and Equine Piroplasmosis, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.Background: Babesiosis represents a veterinary and medical threat, with a need for novel drugs. Artemisinin-based combination therapies (ACT) have been successfully implemented for malaria, a human disease caused by related parasites, Plasmodium spp. The aim of this study was to investigate whether ACT is active against Babesia in vitro and in vivo. Methods: Mefloquine, tafenoquine, primaquine, methylene blue and lumefantrine, alone or in combination with artesunate, were tested in vitro against Babesia bovis. Parasite growth was verified using a SYBR green I-based fluorescence assay. Mice infected with Babesia microti were treated with mefloquine or tafenoquine, alone or in combination with artesunate, and parasitemia was verified by microscopy and PCR. Results: All drugs, except lumefantrine, showed in vitro activity against B. bovis, with methylene blue showing the most potent activity (concentration 0.2 μM). Combination with artesunate led to improved activity, with mefloquine showing a striking 20-fold increase in activity. Tafenoquine (10 mg/kg, base), combined or not with artesunate, but not mefloquine, induced rapid clearance of B. microti in vivo by microscopy, but mice remained PCR-positive. Blood from mice treated with tafenoquine alone, but not with tafenoquine-artesunate, was infective for naive mice upon sub-inoculation. Conclusions: Tafenoquine, and most likely other 8-aminoquinoline compounds, are promising compounds for the development of ACT for babesiosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45543/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLeonardoJ_Carvalho_etal_IOC_2020.pdfLeonardoJ_Carvalho_etal_IOC_2020.pdfapplication/pdf2811468https://www.arca.fiocruz.br/bitstream/icict/45543/2/LeonardoJ_Carvalho_etal_IOC_2020.pdf590b1cd0fd64e9d6ff0f2657482f6b2fMD52TEXTLeonardoJ_Carvalho_etal_IOC_2020.pdf.txtLeonardoJ_Carvalho_etal_IOC_2020.pdf.txtExtracted texttext/plain39302https://www.arca.fiocruz.br/bitstream/icict/45543/3/LeonardoJ_Carvalho_etal_IOC_2020.pdf.txtd311b2bd8e222a41430edcec4b29c58aMD53icict/455432021-03-05 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dc.title.pt_BR.fl_str_mv Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo
title Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo
spellingShingle Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo
Carvalho, Leonardo J. M.
Artesunato
Artesunato
Babesia
Artesunate
Tafenoquine
Artesunato
title_short Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo
title_full Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo
title_fullStr Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo
title_full_unstemmed Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo
title_sort Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo
author Carvalho, Leonardo J. M.
author_facet Carvalho, Leonardo J. M.
Tuvshintulga, Bunduurem
Nugraha, Arifin B.
Sivakumar, Thillaiampalam Sivakumar
Yokoyama, Naoaki
author_role author
author2 Tuvshintulga, Bunduurem
Nugraha, Arifin B.
Sivakumar, Thillaiampalam Sivakumar
Yokoyama, Naoaki
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Leonardo J. M.
Tuvshintulga, Bunduurem
Nugraha, Arifin B.
Sivakumar, Thillaiampalam Sivakumar
Yokoyama, Naoaki
dc.subject.other.pt_BR.fl_str_mv Artesunato
Artesunato
topic Artesunato
Artesunato
Babesia
Artesunate
Tafenoquine
Artesunato
dc.subject.en.pt_BR.fl_str_mv Babesia
Artesunate
Tafenoquine
dc.subject.decs.pt_BR.fl_str_mv Artesunato
description National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan / Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-01-09T18:03:08Z
dc.date.available.fl_str_mv 2021-01-09T18:03:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Leonardo J. M. et al. Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo. Parasites & Vectors, v. 13, n. 362, p. 1-9, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45543
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-020-04235-7
identifier_str_mv CARVALHO, Leonardo J. M. et al. Activities of artesunate‑based combinations and tafenoquine against Babesia bovis in vitro and Babesia microti in vivo. Parasites & Vectors, v. 13, n. 362, p. 1-9, 2020.
1756-3305
10.1186/s13071-020-04235-7
url https://www.arca.fiocruz.br/handle/icict/45543
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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