F1 Domain of the Leishmania (Leishmania) donovani Nucleoside Hydrolase Promotes a Th1 Response in Leishmania (Leishmania) infantum Cured Patients and in Asymptomatic Individuals Living in an Endemic Area of Leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Carrillo, Eugenia
Data de Publicação: 2017
Outros Autores: Fernandez, Laura, Ibarra-Meneses, Ana Victoria, Santos, Micheli L. B., Nico, Dirlei, Luca, Paula M. de, Correa, Cristiane Bani, Almeida, Roque Pacheco de, Moreno, Javier, Sousa, Clarisa B. Palatnik de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23540
Resumo: WHO Collaborating Centre for Leishmaniasis. Centro Nacional de Microbiologia. Instituto de Salud Carlos III. Madrid, Spain.
id CRUZ_bf1fb888c8d9eaf7da83125e907bc70b
oai_identifier_str oai:www.arca.fiocruz.br:icict/23540
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Carrillo, EugeniaFernandez, LauraIbarra-Meneses, Ana VictoriaSantos, Micheli L. B.Nico, DirleiLuca, Paula M. deCorrea, Cristiane BaniAlmeida, Roque Pacheco deMoreno, JavierSousa, Clarisa B. Palatnik de2017-12-07T13:17:53Z2017-12-07T13:17:53Z2017CARRILLO, Eugenia; et al. F1 Domain of the Leishmania (Leishmania) donovani nucleoside hydrolase Promotes a Th1 response in Leishmania (Leishmania) infantum cured Patients and in asymptomatic individuals living in an endemic area of leishmaniasis. Frontiers in Immunology, v.8, Article 750, 13p, July 2017.1664-3224https://www.arca.fiocruz.br/handle/icict/2354010.3389/fimmu.2017.00750engFrontiers MediaLeishmaniose VisceralImunidade AdaptativaLeishmaniose CutâneaEpitopos de Linfócito BEpitopos de Linfócito Thuman visceral leishmaniasiscutaneous leishmaniasisnucleoside hydrolaseB cell epitopesT cell epitopesadaptive immunityF1 Domain of the Leishmania (Leishmania) donovani Nucleoside Hydrolase Promotes a Th1 Response in Leishmania (Leishmania) infantum Cured Patients and in Asymptomatic Individuals Living in an Endemic Area of Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleWHO Collaborating Centre for Leishmaniasis. Centro Nacional de Microbiologia. Instituto de Salud Carlos III. Madrid, Spain.WHO Collaborating Centre for Leishmaniasis. Centro Nacional de Microbiologia. Instituto de Salud Carlos III. Madrid, Spain.WHO Collaborating Centre for Leishmaniasis. Centro Nacional de Microbiologia. Instituto de Salud Carlos III. Madrid, Spain.Universidade Federal de Sergipe. Departamento de Medicina. Hospital Universitário. Aracaju, SE, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.Universidade Federal de Sergipe. Departamento de Morfologia. Aracaju, SE, Brasil.Universidade Federal de Sergipe. Departamento de Medicina. Hospital Universitário. Aracaju, SE, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. São Paulo, SP, Brasil.WHO Collaborating Centre for Leishmaniasis. Centro Nacional de Microbiologia. Instituto de Salud Carlos III. Madrid, Spain.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. São Paulo, SP, Brasil.The Leishmania (Leishmania) donovani nucleoside hydrolase NH36 is the main antigen of the Leishmune® vaccine and one of the promising candidates for vaccination against visceral leishmaniasis. The antigenicity of the N-terminal (F1), the central (F2), or the C-terminal recombinant domain (F3) of NH36 was evaluated using peripheral blood mononuclear cells (PBMC) from individuals infected with L. (L.) infantum from an endemic area of visceral leishmaniasis of Spain. Both NH36 and F1 domains significantly increased the PBMC proliferation stimulation index of cured patients and infected asymptomatic individuals compared to healthy controls. Moreover, F1 induced a 19% higher proliferative response than NH36 in asymptomatic exposed subjects. In addition, in patients cured from visceral leishmaniasis, proliferation in response to NH36 and F1 was accompanied by a significant increase of IFN-γ and TNF-α secretion, which was 42-43% higher, in response to F1 than to NH36. The interleukin 17 (IL-17) secretion was stronger in asymptomatic subjects, in response to F1, as well as in cured cutaneous leishmaniasis after NH36 stimulation. While no IL-10 secretion was determined by F1, a granzyme B increase was detected in supernatants from cured patients after stimulation with either NH36 or F1. These data demonstrate that F1 is the domain of NH36 that induces a recall cellular response in individuals with acquired resistance to the infection by L. (L.) infantum. In addition, F1 and NH36 discriminated the IgG3 humoral response in patients with active visceral leishmaniasis due to L. (L.) donovani (Ethiopia) and L. (L.) infantum (Spain) from that of endemic and non-endemic area controls. NH36 showed higher reactivity with sera from L. (L.) donovani-infected individuals, indicating species specificity. We conclude that the F1 domain, previously characterized as an inducer of the Th1 and Th17 responses in cured/exposed patients infected with L. (L.) infantum chagasi, may also be involved in the generation of a protective response against L. (L.) infantum and represents a potential vaccine candidate for the control of human leishmaniasis alone, or in combination with other HLA epitopes/antigens.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23540/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPaula2_delucca_etal_IOC_2017.pdfPaula2_delucca_etal_IOC_2017.pdfapplication/pdf1441681https://www.arca.fiocruz.br/bitstream/icict/23540/2/Paula2_delucca_etal_IOC_2017.pdf9890c65419679f1d6ff791b30925018fMD52TEXTPaula2_delucca_etal_IOC_2017.pdf.txtPaula2_delucca_etal_IOC_2017.pdf.txtExtracted texttext/plain68796https://www.arca.fiocruz.br/bitstream/icict/23540/3/Paula2_delucca_etal_IOC_2017.pdf.txtf2b68a5287a3026b52508429414091e8MD53icict/235402018-08-15 02:10:00.309oai:www.arca.fiocruz.br:icict/23540Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-08-15T05:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv F1 Domain of the Leishmania (Leishmania) donovani Nucleoside Hydrolase Promotes a Th1 Response in Leishmania (Leishmania) infantum Cured Patients and in Asymptomatic Individuals Living in an Endemic Area of Leishmaniasis
title F1 Domain of the Leishmania (Leishmania) donovani Nucleoside Hydrolase Promotes a Th1 Response in Leishmania (Leishmania) infantum Cured Patients and in Asymptomatic Individuals Living in an Endemic Area of Leishmaniasis
spellingShingle F1 Domain of the Leishmania (Leishmania) donovani Nucleoside Hydrolase Promotes a Th1 Response in Leishmania (Leishmania) infantum Cured Patients and in Asymptomatic Individuals Living in an Endemic Area of Leishmaniasis
Carrillo, Eugenia
Leishmaniose Visceral
Imunidade Adaptativa
Leishmaniose Cutânea
Epitopos de Linfócito B
Epitopos de Linfócito T
human visceral leishmaniasis
cutaneous leishmaniasis
nucleoside hydrolase
B cell epitopes
T cell epitopes
adaptive immunity
title_short F1 Domain of the Leishmania (Leishmania) donovani Nucleoside Hydrolase Promotes a Th1 Response in Leishmania (Leishmania) infantum Cured Patients and in Asymptomatic Individuals Living in an Endemic Area of Leishmaniasis
title_full F1 Domain of the Leishmania (Leishmania) donovani Nucleoside Hydrolase Promotes a Th1 Response in Leishmania (Leishmania) infantum Cured Patients and in Asymptomatic Individuals Living in an Endemic Area of Leishmaniasis
title_fullStr F1 Domain of the Leishmania (Leishmania) donovani Nucleoside Hydrolase Promotes a Th1 Response in Leishmania (Leishmania) infantum Cured Patients and in Asymptomatic Individuals Living in an Endemic Area of Leishmaniasis
title_full_unstemmed F1 Domain of the Leishmania (Leishmania) donovani Nucleoside Hydrolase Promotes a Th1 Response in Leishmania (Leishmania) infantum Cured Patients and in Asymptomatic Individuals Living in an Endemic Area of Leishmaniasis
title_sort F1 Domain of the Leishmania (Leishmania) donovani Nucleoside Hydrolase Promotes a Th1 Response in Leishmania (Leishmania) infantum Cured Patients and in Asymptomatic Individuals Living in an Endemic Area of Leishmaniasis
author Carrillo, Eugenia
author_facet Carrillo, Eugenia
Fernandez, Laura
Ibarra-Meneses, Ana Victoria
Santos, Micheli L. B.
Nico, Dirlei
Luca, Paula M. de
Correa, Cristiane Bani
Almeida, Roque Pacheco de
Moreno, Javier
Sousa, Clarisa B. Palatnik de
author_role author
author2 Fernandez, Laura
Ibarra-Meneses, Ana Victoria
Santos, Micheli L. B.
Nico, Dirlei
Luca, Paula M. de
Correa, Cristiane Bani
Almeida, Roque Pacheco de
Moreno, Javier
Sousa, Clarisa B. Palatnik de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carrillo, Eugenia
Fernandez, Laura
Ibarra-Meneses, Ana Victoria
Santos, Micheli L. B.
Nico, Dirlei
Luca, Paula M. de
Correa, Cristiane Bani
Almeida, Roque Pacheco de
Moreno, Javier
Sousa, Clarisa B. Palatnik de
dc.subject.other.pt_BR.fl_str_mv Leishmaniose Visceral
Imunidade Adaptativa
Leishmaniose Cutânea
Epitopos de Linfócito B
Epitopos de Linfócito T
topic Leishmaniose Visceral
Imunidade Adaptativa
Leishmaniose Cutânea
Epitopos de Linfócito B
Epitopos de Linfócito T
human visceral leishmaniasis
cutaneous leishmaniasis
nucleoside hydrolase
B cell epitopes
T cell epitopes
adaptive immunity
dc.subject.en.pt_BR.fl_str_mv human visceral leishmaniasis
cutaneous leishmaniasis
nucleoside hydrolase
B cell epitopes
T cell epitopes
adaptive immunity
description WHO Collaborating Centre for Leishmaniasis. Centro Nacional de Microbiologia. Instituto de Salud Carlos III. Madrid, Spain.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-12-07T13:17:53Z
dc.date.available.fl_str_mv 2017-12-07T13:17:53Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARRILLO, Eugenia; et al. F1 Domain of the Leishmania (Leishmania) donovani nucleoside hydrolase Promotes a Th1 response in Leishmania (Leishmania) infantum cured Patients and in asymptomatic individuals living in an endemic area of leishmaniasis. Frontiers in Immunology, v.8, Article 750, 13p, July 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23540
dc.identifier.issn.pt_BR.fl_str_mv 1664-3224
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2017.00750
identifier_str_mv CARRILLO, Eugenia; et al. F1 Domain of the Leishmania (Leishmania) donovani nucleoside hydrolase Promotes a Th1 response in Leishmania (Leishmania) infantum cured Patients and in asymptomatic individuals living in an endemic area of leishmaniasis. Frontiers in Immunology, v.8, Article 750, 13p, July 2017.
1664-3224
10.3389/fimmu.2017.00750
url https://www.arca.fiocruz.br/handle/icict/23540
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/23540/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/23540/2/Paula2_delucca_etal_IOC_2017.pdf
https://www.arca.fiocruz.br/bitstream/icict/23540/3/Paula2_delucca_etal_IOC_2017.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
9890c65419679f1d6ff791b30925018f
f2b68a5287a3026b52508429414091e8
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813008902101401600

Registros relacionados