Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain

Detalhes bibliográficos
Autor(a) principal: Rose, Tatiana L.
Data de Publicação: 2010
Outros Autores: Miagostovich, Marize P., Leite, José Paulo G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25409
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
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spelling Rose, Tatiana L.Miagostovich, Marize P.Leite, José Paulo G.2018-03-20T12:00:25Z2018-03-20T12:00:25Z2010ROSE, Tatiana L.; MIAGOSTOVICH, Marize P.; LEITE, José Paulo G. Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain. Mem Inst Oswaldo Cruz, v.105, n.8, p.1068-1072, Dec. 2010.0074-0276https://www.arca.fiocruz.br/handle/icict/254091678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzEstirpe da vacina Rotarix®restriction endonucleaseRotavirus AInfecções por RotavirusRotarix® vaccine strainG1P[8] wild-type strainrotavirus A strain differentiationrestriction endonucleaseRotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine straininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Rotaviruses are important enteric pathogens for humans and animals. Group A rotaviruses (RV-A) are the most common agents of severe gastroenteritis in infants and young children and vaccination is the most effective method to reduce RV-A-associated diseases. G1P[8], the most prevalent RV-A genotype worldwide, is included in the RV-A vaccine Rotarix®. The discrimination between wild-type G1P[8] and vaccine G1P[8] strains is an important topic in the study of RV-A epidemiology to manage outbreaks and to define control measures for vaccinated children. In this study, we developed a novel method to segregate the wild-type and vaccine strains using restriction endonucleases. The dsRNA from the Rotarix® vaccine was sequenced and the NSP3 gene was selected as the target gene. The vaccine strain has a restriction pattern that is different than that of wild-type RV-A G1P[8] isolates after digestion with the restriction endonuclease BspHI. This pattern could be used as a marker for the differentiation of wild-type G1P[8] strains from the vaccine strain.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25409/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALtatiana_rose_etal_IOC_2010.pdftatiana_rose_etal_IOC_2010.pdfapplication/pdf475551https://www.arca.fiocruz.br/bitstream/icict/25409/2/tatiana_rose_etal_IOC_2010.pdf842a18e1b6ca68289424b33870a430a1MD52TEXTtatiana_rose_etal_IOC_2010.pdf.txttatiana_rose_etal_IOC_2010.pdf.txtExtracted texttext/plain25318https://www.arca.fiocruz.br/bitstream/icict/25409/3/tatiana_rose_etal_IOC_2010.pdf.txtef98a3eb2579b2c99feade9bbd5692e4MD53icict/254092018-08-15 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dc.title.pt_BR.fl_str_mv Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain
title Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain
spellingShingle Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain
Rose, Tatiana L.
Estirpe da vacina Rotarix®
restriction endonuclease
Rotavirus A
Infecções por Rotavirus
Rotarix® vaccine strain
G1P[8] wild-type strain
rotavirus A strain differentiation
restriction endonuclease
title_short Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain
title_full Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain
title_fullStr Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain
title_full_unstemmed Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain
title_sort Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain
author Rose, Tatiana L.
author_facet Rose, Tatiana L.
Miagostovich, Marize P.
Leite, José Paulo G.
author_role author
author2 Miagostovich, Marize P.
Leite, José Paulo G.
author2_role author
author
dc.contributor.author.fl_str_mv Rose, Tatiana L.
Miagostovich, Marize P.
Leite, José Paulo G.
dc.subject.other.pt_BR.fl_str_mv Estirpe da vacina Rotarix®
restriction endonuclease
Rotavirus A
Infecções por Rotavirus
topic Estirpe da vacina Rotarix®
restriction endonuclease
Rotavirus A
Infecções por Rotavirus
Rotarix® vaccine strain
G1P[8] wild-type strain
rotavirus A strain differentiation
restriction endonuclease
dc.subject.en.pt_BR.fl_str_mv Rotarix® vaccine strain
G1P[8] wild-type strain
rotavirus A strain differentiation
restriction endonuclease
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-03-20T12:00:25Z
dc.date.available.fl_str_mv 2018-03-20T12:00:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROSE, Tatiana L.; MIAGOSTOVICH, Marize P.; LEITE, José Paulo G. Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain. Mem Inst Oswaldo Cruz, v.105, n.8, p.1068-1072, Dec. 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25409
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv ROSE, Tatiana L.; MIAGOSTOVICH, Marize P.; LEITE, José Paulo G. Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain. Mem Inst Oswaldo Cruz, v.105, n.8, p.1068-1072, Dec. 2010.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/25409
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/25409/2/tatiana_rose_etal_IOC_2010.pdf
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