An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)

Detalhes bibliográficos
Autor(a) principal: Fernandes, Octávio
Data de Publicação: 1996
Outros Autores: Bozza, Marcelo, Pascale, Juan M., Miranda, Antonio B. de, Lopes, Ulisses G., Degrave, Wim M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36812
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
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spelling Fernandes, OctávioBozza, MarceloPascale, Juan M.Miranda, Antonio B. deLopes, Ulisses G.Degrave, Wim M.2019-11-02T19:31:21Z2019-11-02T19:31:21Z1996FERNANDES, Octavio et al. An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 91, n. 3, p. 279-284, May/June 1996.0074-0276https://www.arca.fiocruz.br/handle/icict/3681210.1590/S0074-027619960003000051678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Leishmania braziliensisLeishmania panamensisLeishmania (Viannia) identificationMinicircle DNA sequencesOligonucleotide probeLeishmania braziliensisLeishmania panamensisAn oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Biofísica. Rio de Janeiro, RJ, Brasil.Universidade do Panamá. Departamento de Parasitologia. Panamá.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Biofísica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Sequence analysis of Leishmania (Viannia) kDNA minicircles and analysis of multiple sequence alignments of the conserved region (minirepeats) of five distinct minicircles from L. (V.) braziliensis species with corresponding sequences derived from other dermotropic leishmanias indicated the presence of a sub-genus specific sequence. An oligonucleotide bearing this sequence was designed and used as a molecular probe, being able to recognize solely the sub-genus Viannia species in hybridization experiments. A dendrogram reflecting the homologies among the minirepeat sequences was constructed. Sequence clustering was obtained corresponding to the traditional classification based on similarity of biochemical, biological and parasitological characteristics of these Leishmania species, distinguishing the Old World dermotropic leishmanias, the New World dermotropic leishmanias of the sub-genus Leishmania and of the sub-genus Viannia.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
title An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
spellingShingle An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
Fernandes, Octávio
Leishmania braziliensis
Leishmania panamensis
Leishmania (Viannia) identification
Minicircle DNA sequences
Oligonucleotide probe
Leishmania braziliensis
Leishmania panamensis
title_short An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
title_full An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
title_fullStr An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
title_full_unstemmed An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
title_sort An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
author Fernandes, Octávio
author_facet Fernandes, Octávio
Bozza, Marcelo
Pascale, Juan M.
Miranda, Antonio B. de
Lopes, Ulisses G.
Degrave, Wim M.
author_role author
author2 Bozza, Marcelo
Pascale, Juan M.
Miranda, Antonio B. de
Lopes, Ulisses G.
Degrave, Wim M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Octávio
Bozza, Marcelo
Pascale, Juan M.
Miranda, Antonio B. de
Lopes, Ulisses G.
Degrave, Wim M.
dc.subject.other.pt_BR.fl_str_mv Leishmania braziliensis
Leishmania panamensis
topic Leishmania braziliensis
Leishmania panamensis
Leishmania (Viannia) identification
Minicircle DNA sequences
Oligonucleotide probe
Leishmania braziliensis
Leishmania panamensis
dc.subject.en.pt_BR.fl_str_mv Leishmania (Viannia) identification
Minicircle DNA sequences
Oligonucleotide probe
Leishmania braziliensis
Leishmania panamensis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
publishDate 1996
dc.date.issued.fl_str_mv 1996
dc.date.accessioned.fl_str_mv 2019-11-02T19:31:21Z
dc.date.available.fl_str_mv 2019-11-02T19:31:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERNANDES, Octavio et al. An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 91, n. 3, p. 279-284, May/June 1996.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36812
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761996000300005
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv FERNANDES, Octavio et al. An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 91, n. 3, p. 279-284, May/June 1996.
0074-0276
10.1590/S0074-02761996000300005
1678-8060
url https://www.arca.fiocruz.br/handle/icict/36812
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/36812/3/OctavioFernandes_WinDegrave_etal_IOC_1996.pdf.txt
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