An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)

Detalhes bibliográficos
Autor(a) principal: Fernandes,Octavio
Data de Publicação: 1996
Outros Autores: Bozza,Marcelo, Pascale,Juan M, Miranda,Antonio B de, Lopes,Ulisses G, Degrave,Wim M
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000300005
Resumo: Sequence analysis of Leishmania (Viannia) kDNA minicircles and analysis of multiple sequence alignments of the conserved region (minirepeats) of five distinct minicircles from L. (V.) braziliensis species with corresponding sequences derived from other dermotropic leishmanias indicated the presence of a sub-genus specific sequence. An oligonucleotide bearing this sequence was designed and used as a molecular probe, being able to recognize solely the sub-genus Viannia species in hybridization experiments. A dendrogram reflecting the homologies among the minirepeat sequences was constructed. Sequence clustering was obtained corresponding to the traditional classification based on similarity of biochemical, biological and parasitological characteristics of these Leishmania species, distinguishing the Old World dermotropic leishmanias, the New World dermotropic leishmanias of the sub-genus Leishmania and of the sub-genus Viannia.
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spelling An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)Leishmania (Viannia) identificationminicircle DNA sequencesoligonucleotide probeLeishmania braziliensisLeishmania panamensisSequence analysis of Leishmania (Viannia) kDNA minicircles and analysis of multiple sequence alignments of the conserved region (minirepeats) of five distinct minicircles from L. (V.) braziliensis species with corresponding sequences derived from other dermotropic leishmanias indicated the presence of a sub-genus specific sequence. An oligonucleotide bearing this sequence was designed and used as a molecular probe, being able to recognize solely the sub-genus Viannia species in hybridization experiments. A dendrogram reflecting the homologies among the minirepeat sequences was constructed. Sequence clustering was obtained corresponding to the traditional classification based on similarity of biochemical, biological and parasitological characteristics of these Leishmania species, distinguishing the Old World dermotropic leishmanias, the New World dermotropic leishmanias of the sub-genus Leishmania and of the sub-genus Viannia.Instituto Oswaldo Cruz, Ministério da Saúde1996-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000300005Memórias do Instituto Oswaldo Cruz v.91 n.3 1996reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761996000300005info:eu-repo/semantics/openAccessFernandes,OctavioBozza,MarceloPascale,Juan MMiranda,Antonio B deLopes,Ulisses GDegrave,Wim Meng2020-04-25T17:47:33Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:06:59.224Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
title An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
spellingShingle An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
Fernandes,Octavio
Leishmania (Viannia) identification
minicircle DNA sequences
oligonucleotide probe
Leishmania braziliensis
Leishmania panamensis
title_short An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
title_full An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
title_fullStr An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
title_full_unstemmed An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
title_sort An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
author Fernandes,Octavio
author_facet Fernandes,Octavio
Bozza,Marcelo
Pascale,Juan M
Miranda,Antonio B de
Lopes,Ulisses G
Degrave,Wim M
author_role author
author2 Bozza,Marcelo
Pascale,Juan M
Miranda,Antonio B de
Lopes,Ulisses G
Degrave,Wim M
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes,Octavio
Bozza,Marcelo
Pascale,Juan M
Miranda,Antonio B de
Lopes,Ulisses G
Degrave,Wim M
dc.subject.por.fl_str_mv Leishmania (Viannia) identification
minicircle DNA sequences
oligonucleotide probe
Leishmania braziliensis
Leishmania panamensis
topic Leishmania (Viannia) identification
minicircle DNA sequences
oligonucleotide probe
Leishmania braziliensis
Leishmania panamensis
dc.description.none.fl_txt_mv Sequence analysis of Leishmania (Viannia) kDNA minicircles and analysis of multiple sequence alignments of the conserved region (minirepeats) of five distinct minicircles from L. (V.) braziliensis species with corresponding sequences derived from other dermotropic leishmanias indicated the presence of a sub-genus specific sequence. An oligonucleotide bearing this sequence was designed and used as a molecular probe, being able to recognize solely the sub-genus Viannia species in hybridization experiments. A dendrogram reflecting the homologies among the minirepeat sequences was constructed. Sequence clustering was obtained corresponding to the traditional classification based on similarity of biochemical, biological and parasitological characteristics of these Leishmania species, distinguishing the Old World dermotropic leishmanias, the New World dermotropic leishmanias of the sub-genus Leishmania and of the sub-genus Viannia.
description Sequence analysis of Leishmania (Viannia) kDNA minicircles and analysis of multiple sequence alignments of the conserved region (minirepeats) of five distinct minicircles from L. (V.) braziliensis species with corresponding sequences derived from other dermotropic leishmanias indicated the presence of a sub-genus specific sequence. An oligonucleotide bearing this sequence was designed and used as a molecular probe, being able to recognize solely the sub-genus Viannia species in hybridization experiments. A dendrogram reflecting the homologies among the minirepeat sequences was constructed. Sequence clustering was obtained corresponding to the traditional classification based on similarity of biochemical, biological and parasitological characteristics of these Leishmania species, distinguishing the Old World dermotropic leishmanias, the New World dermotropic leishmanias of the sub-genus Leishmania and of the sub-genus Viannia.
publishDate 1996
dc.date.none.fl_str_mv 1996-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000300005
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000300005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761996000300005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.91 n.3 1996
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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