Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/27522 |
Resumo: | Fundação Oswaldo Cruz. Porto Velho, RO, Brasil. |
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Prado, Nidiane D. R.Pereira, Soraya dos Santos.Silva, Michele P. daMorais, Michelle S. S.Kayano, Anderson M.Moreira-Dill, Leandro Soares.Luiz, Marcos Barros.Zanchi, Fernando Berton.Fuly, André L.Huacca, Maribel E. F.Fernandes, Cleberson F.Calderon, Leonardo de Azevedo.Zuliani, Juliana Pavan.Silva, Luiz H. Pereira daSoares, Andreimar Martins.Stabeli, Rodrigo Guerino.Fernandes, Carla Freire Celedônio.2018-07-12T20:22:29Z2018-07-12T20:22:29Z2016SOARES, A. M. et al. Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments. PLoS One, v. 11, p.1-22 , 2016.https://www.arca.fiocruz.br/handle/icict/275221932-6203engPlos OneInhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Universidade Federal Fluminense. Rio de Janeiro, RJ, Brasil.Universidade Federal de Rondônia. Porto Velho, RO, Brasil.Empresa Brasileira de Pesquisa Agropecuária. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil. / Universidade Federal de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil. / Universidade Federal de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil. / Universidade Federal de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil. / Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Antivenoms, produced using animal hyperimmune plasma, remains the standard therapy for snakebites. Although effective against systemic damages, conventional antivenoms have limited efficacy against local tissue damage. Additionally, the hypersensitivity reactions, often elicited by antivenoms, the high costs for animal maintenance, the difficulty of producing homogeneous lots, and the instability of biological products instigate the search for innovative products for antivenomtherapy. In this study, camelid antibody fragments (VHH) with specificity to Bothropstoxin I and II (BthTX-I and BthTX-II), two myotoxic phospholipases from Bothrops jararacussu venom, were selected from an immune VHH phage display library. After biopanning, 28 and 6 clones recognized BthTX-I and BthTX-II by ELISA, respectively. Complementarity determining regions (CDRs) and immunoglobulin frameworks (FRs) of 13 VHH-deduced amino acid sequences were identified, as well as the camelid hallmark amino acid substitutions in FR2. Three VHH clones (KF498607, KF498608, and KC329718) were capable of recognizing BthTX-I byWestern blot and showed affinity constants in the nanomolar range against both toxins. VHHs inhibited the BthTX-II phospholipase A2 activity, and when tested for cross-reactivity, presented specificity to the Bothrops genus in ELISA. Furthermore, two clones (KC329718 and KF498607) neutralized the myotoxic effects induced by B. jararacussu venom, BthTX-I, BthTX-II, and by a myotoxin from Bothrops brazili venom (MTX-I) inmice. Molecular docking revealed that VHH CDRs are expected to bind the C-terminal of both toxins, essential for myotoxic activity, and to epitopes in the BthTX-II enzymatic cleft. Identified VHHs could be a biotechnological tool to improve the treatment for snake envenomation, an important and neglected world public health problem.MyotoxicityBothrops jararacussuPhospholipase A2Antibodyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27522/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALInhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments.PDFInhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments.PDFapplication/pdf2085939https://www.arca.fiocruz.br/bitstream/icict/27522/2/Inhibition%20of%20the%20Myotoxicity%20Induced%20by%20Bothrops%20jararacussu%20Venom%20and%20Isolated%20Phospholipases%20A2%20by%20Specific%20Camelid%20Single-Domain%20Antibody%20Fragments.PDFee0b2f63f746338bb86bb2d5e895816dMD52TEXTInhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments.PDF.txtInhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments.PDF.txtExtracted texttext/plain69871https://www.arca.fiocruz.br/bitstream/icict/27522/3/Inhibition%20of%20the%20Myotoxicity%20Induced%20by%20Bothrops%20jararacussu%20Venom%20and%20Isolated%20Phospholipases%20A2%20by%20Specific%20Camelid%20Single-Domain%20Antibody%20Fragments.PDF.txtbb5f86921c9e18a0140350453a683cd1MD53icict/275222018-08-15 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| dc.title.pt_BR.fl_str_mv |
Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments. |
| title |
Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments. |
| spellingShingle |
Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments. Prado, Nidiane D. R. Myotoxicity Bothrops jararacussu Phospholipase A2 Antibody |
| title_short |
Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments. |
| title_full |
Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments. |
| title_fullStr |
Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments. |
| title_full_unstemmed |
Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments. |
| title_sort |
Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments. |
| author |
Prado, Nidiane D. R. |
| author_facet |
Prado, Nidiane D. R. Pereira, Soraya dos Santos. Silva, Michele P. da Morais, Michelle S. S. Kayano, Anderson M. Moreira-Dill, Leandro Soares. Luiz, Marcos Barros. Zanchi, Fernando Berton. Fuly, André L. Huacca, Maribel E. F. Fernandes, Cleberson F. Calderon, Leonardo de Azevedo. Zuliani, Juliana Pavan. Silva, Luiz H. Pereira da Soares, Andreimar Martins. Stabeli, Rodrigo Guerino. Fernandes, Carla Freire Celedônio. |
| author_role |
author |
| author2 |
Pereira, Soraya dos Santos. Silva, Michele P. da Morais, Michelle S. S. Kayano, Anderson M. Moreira-Dill, Leandro Soares. Luiz, Marcos Barros. Zanchi, Fernando Berton. Fuly, André L. Huacca, Maribel E. F. Fernandes, Cleberson F. Calderon, Leonardo de Azevedo. Zuliani, Juliana Pavan. Silva, Luiz H. Pereira da Soares, Andreimar Martins. Stabeli, Rodrigo Guerino. Fernandes, Carla Freire Celedônio. |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Prado, Nidiane D. R. Pereira, Soraya dos Santos. Silva, Michele P. da Morais, Michelle S. S. Kayano, Anderson M. Moreira-Dill, Leandro Soares. Luiz, Marcos Barros. Zanchi, Fernando Berton. Fuly, André L. Huacca, Maribel E. F. Fernandes, Cleberson F. Calderon, Leonardo de Azevedo. Zuliani, Juliana Pavan. Silva, Luiz H. Pereira da Soares, Andreimar Martins. Stabeli, Rodrigo Guerino. Fernandes, Carla Freire Celedônio. |
| dc.subject.en.pt_BR.fl_str_mv |
Myotoxicity Bothrops jararacussu Phospholipase A2 Antibody |
| topic |
Myotoxicity Bothrops jararacussu Phospholipase A2 Antibody |
| description |
Fundação Oswaldo Cruz. Porto Velho, RO, Brasil. |
| publishDate |
2016 |
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2016 |
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2018-07-12T20:22:29Z |
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2018-07-12T20:22:29Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SOARES, A. M. et al. Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments. PLoS One, v. 11, p.1-22 , 2016. |
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https://www.arca.fiocruz.br/handle/icict/27522 |
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1932-6203 |
| identifier_str_mv |
SOARES, A. M. et al. Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments. PLoS One, v. 11, p.1-22 , 2016. 1932-6203 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Plos One |
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Plos One |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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