Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.

Detalhes bibliográficos
Autor(a) principal: Prado, Nidiane D. R.
Data de Publicação: 2016
Outros Autores: Pereira, Soraya dos Santos., Silva, Michele P. da, Morais, Michelle S. S., Kayano, Anderson M., Moreira-Dill, Leandro Soares., Luiz, Marcos Barros., Zanchi, Fernando Berton., Fuly, André L., Huacca, Maribel E. F., Fernandes, Cleberson F., Calderon, Leonardo de Azevedo., Zuliani, Juliana Pavan., Silva, Luiz H. Pereira da, Soares, Andreimar Martins., Stabeli, Rodrigo Guerino., Fernandes, Carla Freire Celedônio.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27522
Resumo: Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.
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spelling Prado, Nidiane D. R.Pereira, Soraya dos Santos.Silva, Michele P. daMorais, Michelle S. S.Kayano, Anderson M.Moreira-Dill, Leandro Soares.Luiz, Marcos Barros.Zanchi, Fernando Berton.Fuly, André L.Huacca, Maribel E. F.Fernandes, Cleberson F.Calderon, Leonardo de Azevedo.Zuliani, Juliana Pavan.Silva, Luiz H. Pereira daSoares, Andreimar Martins.Stabeli, Rodrigo Guerino.Fernandes, Carla Freire Celedônio.2018-07-12T20:22:29Z2018-07-12T20:22:29Z2016SOARES, A. M. et al. Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments. PLoS One, v. 11, p.1-22 , 2016.https://www.arca.fiocruz.br/handle/icict/275221932-6203engPlos OneInhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Universidade Federal Fluminense. Rio de Janeiro, RJ, Brasil.Universidade Federal de Rondônia. Porto Velho, RO, Brasil.Empresa Brasileira de Pesquisa Agropecuária. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil. / Universidade Federal de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil. / Universidade Federal de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil. / Universidade Federal de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Porto Velho, RO, Brasil. / Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Antivenoms, produced using animal hyperimmune plasma, remains the standard therapy for snakebites. Although effective against systemic damages, conventional antivenoms have limited efficacy against local tissue damage. Additionally, the hypersensitivity reactions, often elicited by antivenoms, the high costs for animal maintenance, the difficulty of producing homogeneous lots, and the instability of biological products instigate the search for innovative products for antivenomtherapy. In this study, camelid antibody fragments (VHH) with specificity to Bothropstoxin I and II (BthTX-I and BthTX-II), two myotoxic phospholipases from Bothrops jararacussu venom, were selected from an immune VHH phage display library. After biopanning, 28 and 6 clones recognized BthTX-I and BthTX-II by ELISA, respectively. Complementarity determining regions (CDRs) and immunoglobulin frameworks (FRs) of 13 VHH-deduced amino acid sequences were identified, as well as the camelid hallmark amino acid substitutions in FR2. Three VHH clones (KF498607, KF498608, and KC329718) were capable of recognizing BthTX-I byWestern blot and showed affinity constants in the nanomolar range against both toxins. VHHs inhibited the BthTX-II phospholipase A2 activity, and when tested for cross-reactivity, presented specificity to the Bothrops genus in ELISA. Furthermore, two clones (KC329718 and KF498607) neutralized the myotoxic effects induced by B. jararacussu venom, BthTX-I, BthTX-II, and by a myotoxin from Bothrops brazili venom (MTX-I) inmice. Molecular docking revealed that VHH CDRs are expected to bind the C-terminal of both toxins, essential for myotoxic activity, and to epitopes in the BthTX-II enzymatic cleft. Identified VHHs could be a biotechnological tool to improve the treatment for snake envenomation, an important and neglected world public health problem.MyotoxicityBothrops jararacussuPhospholipase A2Antibodyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27522/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALInhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments.PDFInhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments.PDFapplication/pdf2085939https://www.arca.fiocruz.br/bitstream/icict/27522/2/Inhibition%20of%20the%20Myotoxicity%20Induced%20by%20Bothrops%20jararacussu%20Venom%20and%20Isolated%20Phospholipases%20A2%20by%20Specific%20Camelid%20Single-Domain%20Antibody%20Fragments.PDFee0b2f63f746338bb86bb2d5e895816dMD52TEXTInhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments.PDF.txtInhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments.PDF.txtExtracted texttext/plain69871https://www.arca.fiocruz.br/bitstream/icict/27522/3/Inhibition%20of%20the%20Myotoxicity%20Induced%20by%20Bothrops%20jararacussu%20Venom%20and%20Isolated%20Phospholipases%20A2%20by%20Specific%20Camelid%20Single-Domain%20Antibody%20Fragments.PDF.txtbb5f86921c9e18a0140350453a683cd1MD53icict/275222018-08-15 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dc.title.pt_BR.fl_str_mv Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.
title Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.
spellingShingle Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.
Prado, Nidiane D. R.
Myotoxicity
Bothrops jararacussu
Phospholipase A2
Antibody
title_short Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.
title_full Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.
title_fullStr Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.
title_full_unstemmed Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.
title_sort Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single- Domain Antibody Fragments.
author Prado, Nidiane D. R.
author_facet Prado, Nidiane D. R.
Pereira, Soraya dos Santos.
Silva, Michele P. da
Morais, Michelle S. S.
Kayano, Anderson M.
Moreira-Dill, Leandro Soares.
Luiz, Marcos Barros.
Zanchi, Fernando Berton.
Fuly, André L.
Huacca, Maribel E. F.
Fernandes, Cleberson F.
Calderon, Leonardo de Azevedo.
Zuliani, Juliana Pavan.
Silva, Luiz H. Pereira da
Soares, Andreimar Martins.
Stabeli, Rodrigo Guerino.
Fernandes, Carla Freire Celedônio.
author_role author
author2 Pereira, Soraya dos Santos.
Silva, Michele P. da
Morais, Michelle S. S.
Kayano, Anderson M.
Moreira-Dill, Leandro Soares.
Luiz, Marcos Barros.
Zanchi, Fernando Berton.
Fuly, André L.
Huacca, Maribel E. F.
Fernandes, Cleberson F.
Calderon, Leonardo de Azevedo.
Zuliani, Juliana Pavan.
Silva, Luiz H. Pereira da
Soares, Andreimar Martins.
Stabeli, Rodrigo Guerino.
Fernandes, Carla Freire Celedônio.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Prado, Nidiane D. R.
Pereira, Soraya dos Santos.
Silva, Michele P. da
Morais, Michelle S. S.
Kayano, Anderson M.
Moreira-Dill, Leandro Soares.
Luiz, Marcos Barros.
Zanchi, Fernando Berton.
Fuly, André L.
Huacca, Maribel E. F.
Fernandes, Cleberson F.
Calderon, Leonardo de Azevedo.
Zuliani, Juliana Pavan.
Silva, Luiz H. Pereira da
Soares, Andreimar Martins.
Stabeli, Rodrigo Guerino.
Fernandes, Carla Freire Celedônio.
dc.subject.en.pt_BR.fl_str_mv Myotoxicity
Bothrops jararacussu
Phospholipase A2
Antibody
topic Myotoxicity
Bothrops jararacussu
Phospholipase A2
Antibody
description Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2018-07-12T20:22:29Z
dc.date.available.fl_str_mv 2018-07-12T20:22:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOARES, A. M. et al. Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments. PLoS One, v. 11, p.1-22 , 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27522
dc.identifier.eissn.none.fl_str_mv 1932-6203
identifier_str_mv SOARES, A. M. et al. Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments. PLoS One, v. 11, p.1-22 , 2016.
1932-6203
url https://www.arca.fiocruz.br/handle/icict/27522
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/27522/1/license.txt
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https://www.arca.fiocruz.br/bitstream/icict/27522/3/Inhibition%20of%20the%20Myotoxicity%20Induced%20by%20Bothrops%20jararacussu%20Venom%20and%20Isolated%20Phospholipases%20A2%20by%20Specific%20Camelid%20Single-Domain%20Antibody%20Fragments.PDF.txt
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