Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/01480545.2018.1478850 http://hdl.handle.net/11449/171193 |
Resumo: | Nanoparticle-conjugated venom-toxins of venomous animals and its therapeutic efficacy against emerging or neglecting diseases is a promising strategy. In this study, silver nanoparticles (AgNPs ∼50 nm, 0.081 mg mL−1) were studied against the neuromuscular blockade, myotoxic effects induced by Bothrops jararacussu venom (60 µg mL−1) and also against prokaryotic cells. The neurotoxicity was evaluated on ex vivo mouse phrenic nerve-diaphragm using traditional myographic technique, able to obtain functional contractile responses and to check the neurotransmission. The myotoxicity on mammalian cells was evaluated in muscles resulting from pharmacological assays using routine histological techniques and light microscopy. The toxicity to prokaryotic cells was evaluated on Salmonella typhimurium TA100 without metabolic activation. The in vitro preincubation model between AgNPs and venom was enough to abolish toxic effects of B. jararacussu venom, but mammalian cells were highly sensitive to AgNPs more than prokaryotic cells, by acting as dose-independently and dose-dependently parameters, respectively. These results allowed us to conclude that AgNPs showed promising activity as antivenom agent but for its safer use, the toxicity should be evaluated on experimental animals. |
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Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venomBothrops jararacussu venommyotoxicityneurotoxicitySalmonella typhimuriumsilver nanoparticlesNanoparticle-conjugated venom-toxins of venomous animals and its therapeutic efficacy against emerging or neglecting diseases is a promising strategy. In this study, silver nanoparticles (AgNPs ∼50 nm, 0.081 mg mL−1) were studied against the neuromuscular blockade, myotoxic effects induced by Bothrops jararacussu venom (60 µg mL−1) and also against prokaryotic cells. The neurotoxicity was evaluated on ex vivo mouse phrenic nerve-diaphragm using traditional myographic technique, able to obtain functional contractile responses and to check the neurotransmission. The myotoxicity on mammalian cells was evaluated in muscles resulting from pharmacological assays using routine histological techniques and light microscopy. The toxicity to prokaryotic cells was evaluated on Salmonella typhimurium TA100 without metabolic activation. The in vitro preincubation model between AgNPs and venom was enough to abolish toxic effects of B. jararacussu venom, but mammalian cells were highly sensitive to AgNPs more than prokaryotic cells, by acting as dose-independently and dose-dependently parameters, respectively. These results allowed us to conclude that AgNPs showed promising activity as antivenom agent but for its safer use, the toxicity should be evaluated on experimental animals.Veterinary Medicine Graduate Course, University of Sorocaba (UNISO), Sorocaba, BrazilBioprocess and Biotechnology Engineering Course, University of Sorocaba (UNISO), Sorocaba, BrazilPost-Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, BrazilBioengineering and Biomedical Engineering Programs, Technological and Scientific Institute, Brazil University, Itaquera, São Paulo, BrazilPharmaceutical Sciences Faculty of Araraquara, São Paulo State University (UNESP), Araraquara, BrazilDepartment of Biotechnology, SGB Amravati University, Amravati, IndiaUniversidade Estadual Paulista (Unesp)Oliveira, Isadora Caruso Fontanade Paula, Marina OliveiraLastra, Hellen Cristine BoschilhaAlves, Bruno de BritoMoreno, Débora Antunes NetoYoshida, Edson HideakiAmaral Filho, JorgeCogo, José CarlosVaranda, Eliana AparecidaRai, MahendraSantos, Carolina Alves dosOshima-Franco, Yoko2018-12-11T16:54:19Z2018-12-11T16:54:19Z2018-06-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-5http://dx.doi.org/10.1080/01480545.2018.1478850Drug and Chemical Toxicology, p. 1-5.1525-60140148-0545http://hdl.handle.net/11449/17119310.1080/01480545.2018.14788502-s2.0-85049605363Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDrug and Chemical Toxicology0,4600,460info:eu-repo/semantics/openAccess2021-10-23T17:09:04Zoai:repositorio.unesp.br:11449/171193Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:09:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom |
title |
Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom |
spellingShingle |
Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom Oliveira, Isadora Caruso Fontana Bothrops jararacussu venom myotoxicity neurotoxicity Salmonella typhimurium silver nanoparticles |
title_short |
Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom |
title_full |
Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom |
title_fullStr |
Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom |
title_full_unstemmed |
Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom |
title_sort |
Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom |
author |
Oliveira, Isadora Caruso Fontana |
author_facet |
Oliveira, Isadora Caruso Fontana de Paula, Marina Oliveira Lastra, Hellen Cristine Boschilha Alves, Bruno de Brito Moreno, Débora Antunes Neto Yoshida, Edson Hideaki Amaral Filho, Jorge Cogo, José Carlos Varanda, Eliana Aparecida Rai, Mahendra Santos, Carolina Alves dos Oshima-Franco, Yoko |
author_role |
author |
author2 |
de Paula, Marina Oliveira Lastra, Hellen Cristine Boschilha Alves, Bruno de Brito Moreno, Débora Antunes Neto Yoshida, Edson Hideaki Amaral Filho, Jorge Cogo, José Carlos Varanda, Eliana Aparecida Rai, Mahendra Santos, Carolina Alves dos Oshima-Franco, Yoko |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Oliveira, Isadora Caruso Fontana de Paula, Marina Oliveira Lastra, Hellen Cristine Boschilha Alves, Bruno de Brito Moreno, Débora Antunes Neto Yoshida, Edson Hideaki Amaral Filho, Jorge Cogo, José Carlos Varanda, Eliana Aparecida Rai, Mahendra Santos, Carolina Alves dos Oshima-Franco, Yoko |
dc.subject.por.fl_str_mv |
Bothrops jararacussu venom myotoxicity neurotoxicity Salmonella typhimurium silver nanoparticles |
topic |
Bothrops jararacussu venom myotoxicity neurotoxicity Salmonella typhimurium silver nanoparticles |
description |
Nanoparticle-conjugated venom-toxins of venomous animals and its therapeutic efficacy against emerging or neglecting diseases is a promising strategy. In this study, silver nanoparticles (AgNPs ∼50 nm, 0.081 mg mL−1) were studied against the neuromuscular blockade, myotoxic effects induced by Bothrops jararacussu venom (60 µg mL−1) and also against prokaryotic cells. The neurotoxicity was evaluated on ex vivo mouse phrenic nerve-diaphragm using traditional myographic technique, able to obtain functional contractile responses and to check the neurotransmission. The myotoxicity on mammalian cells was evaluated in muscles resulting from pharmacological assays using routine histological techniques and light microscopy. The toxicity to prokaryotic cells was evaluated on Salmonella typhimurium TA100 without metabolic activation. The in vitro preincubation model between AgNPs and venom was enough to abolish toxic effects of B. jararacussu venom, but mammalian cells were highly sensitive to AgNPs more than prokaryotic cells, by acting as dose-independently and dose-dependently parameters, respectively. These results allowed us to conclude that AgNPs showed promising activity as antivenom agent but for its safer use, the toxicity should be evaluated on experimental animals. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:54:19Z 2018-12-11T16:54:19Z 2018-06-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/01480545.2018.1478850 Drug and Chemical Toxicology, p. 1-5. 1525-6014 0148-0545 http://hdl.handle.net/11449/171193 10.1080/01480545.2018.1478850 2-s2.0-85049605363 |
url |
http://dx.doi.org/10.1080/01480545.2018.1478850 http://hdl.handle.net/11449/171193 |
identifier_str_mv |
Drug and Chemical Toxicology, p. 1-5. 1525-6014 0148-0545 10.1080/01480545.2018.1478850 2-s2.0-85049605363 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Drug and Chemical Toxicology 0,460 0,460 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-5 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047457998766080 |