Leishmania Parasites Drive PD-L1 Expression in Mice and Human Neutrophils With Suppressor Capacity
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/47929 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Goes. Departamento de Imunologia. Laboratório de Imunobiologia das Leishmanioses. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Laborato´rio de Imunobiotecnologia. Rio de Janeiro, RJ, Brasil. |
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Martins, Alessandra M. da FonsecaGomes, Phillipe de Souza LimaAntunes, Maísa MotaMoura, Renan Garcia deCovre, Luciana P.Calôba, CarolinaRocha, Vivian GrizentePereira, Renata M.Menezes, Gustavo BatistaGomes, Daniel Claudio OliveiraSaraiva, Elvira M.Guedes, Herbet L. de Matos2021-06-30T14:11:48Z2021-06-30T14:11:48Z2021MARTINS, Alessandra M. da Fonseca et al. Leishmania parasites drive PD-L1 expression in mice and human neutrophils with suppressor capacity. Frontiers in Immunology, v. 12, Article 598943, 15 p, June 2021.1664-3224https://www.arca.fiocruz.br/handle/icict/4792910.3389/fimmu.2021.598943engFrontiers MediaPD-L1NeutrófilosLeishmaniaPeleLeishmaniose cutânea humanaLeishmaniose murinaHibridização Subtrativa por SupressãoPD-L1NeutrophilsSkinLeishmaniaHuman cutaneous leishmaniasisMurine leishmaniasisSupressionTécnicas de Hibridização SubtrativaHibridização Subtrativa por SupressãoLeishmania Parasites Drive PD-L1 Expression in Mice and Human Neutrophils With Suppressor Capacityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Goes. Departamento de Imunologia. Laboratório de Imunobiologia das Leishmanioses. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Laborato´rio de Imunobiotecnologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Goes. Departamento de Imunologia. Laboratório de Imunobiologia das Leishmanioses. Rio de Janeiro, RJ, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestinal. Belo Horizonte, MG, Brasil. . Belo Horizonte, MG, Brasil.Universidade Federal do Espírito Santo. Nu´ cleo de Doenças Infecciosas. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Nu´ cleo de Doenças Infecciosas. Vitória, ES, Brasil / Division of Medicine, University College London. London, United Kingdom.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunobiologia. Laboratório de Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunobiologia. Laboratório de Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunobiologia. Laboratório de Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciencias Biológicas. Departamento de Morfologia. Centro de Biologia Gastrointestina. Belo Horizonte, MG, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Goes. Departamento de Imunologia. Laboratório de Imunobiologia das Leishmanioses. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Laboratório de Imunobiotecnologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinas de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Neutrophils play an important role in the outcome of leishmaniasis, contributing either to exacerbating or controlling the progression of infection, a dual effect whose underlying mechanisms are not clear. We recently reported that CD4+ and CD8+ T cells, and dendritic cells of Leishmania amazonensis-infected mice present high expression of PD-1 and PD-L1, respectively. Given that the PD-1/PD-L1 interaction may promote cellular dysfunction, and that neutrophils could interact with T cells during infection, we investigated here the levels of PD-L1 in neutrophils exposed to Leishmania parasites. We found that both, promastigotes and amastigotes of L. amazonensis induced the expression of PD-L1 in the human and murine neutrophils that internalized these parasites in vitro. PD-L1-expressing neutrophils were also observed in the ear lesions and the draining lymph nodes of L. amazonensis-infected mice, assessed through cell cytometry and intravital microscopy. Moreover, expression of PD-L1 progressively increased in neutrophils from ear lesions as the disease evolved to the chronic phase. Co-culture of infected neutrophils with in vitro activated CD8+ T cells inhibits IFN-g production by a mechanism dependent on PD-1 and PD-L1. Importantly, we demonstrated that in vitro infection of human neutrophils by L braziliensis induced PD-L1+ expression and also PD-L1+ neutrophils were detected in the lesions of patients with cutaneous leishmaniasis. Taken together, these findings suggest that the Leishmania parasite increases the expression of PD-L1 in neutrophils with suppressor capacity, which could favor the parasite survival through impairing the immune response.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/47929/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALHernertMGuedes_etal_IOC_2021.pdfHernertMGuedes_etal_IOC_2021.pdfapplication/pdf3053643https://www.arca.fiocruz.br/bitstream/icict/47929/2/HernertMGuedes_etal_IOC_2021.pdf1c337d93f336f5198941ee69caad5cddMD52TEXTHernertMGuedes_etal_IOC_2021.pdf.txtHernertMGuedes_etal_IOC_2021.pdf.txtExtracted texttext/plain77238https://www.arca.fiocruz.br/bitstream/icict/47929/3/HernertMGuedes_etal_IOC_2021.pdf.txt75fd8ff8aac38cd01a501c504fce5147MD53icict/479292021-07-01 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| dc.title.pt_BR.fl_str_mv |
Leishmania Parasites Drive PD-L1 Expression in Mice and Human Neutrophils With Suppressor Capacity |
| title |
Leishmania Parasites Drive PD-L1 Expression in Mice and Human Neutrophils With Suppressor Capacity |
| spellingShingle |
Leishmania Parasites Drive PD-L1 Expression in Mice and Human Neutrophils With Suppressor Capacity Martins, Alessandra M. da Fonseca PD-L1 Neutrófilos Leishmania Pele Leishmaniose cutânea humana Leishmaniose murina Hibridização Subtrativa por Supressão PD-L1 Neutrophils Skin Leishmania Human cutaneous leishmaniasis Murine leishmaniasis Supression Técnicas de Hibridização Subtrativa Hibridização Subtrativa por Supressão |
| title_short |
Leishmania Parasites Drive PD-L1 Expression in Mice and Human Neutrophils With Suppressor Capacity |
| title_full |
Leishmania Parasites Drive PD-L1 Expression in Mice and Human Neutrophils With Suppressor Capacity |
| title_fullStr |
Leishmania Parasites Drive PD-L1 Expression in Mice and Human Neutrophils With Suppressor Capacity |
| title_full_unstemmed |
Leishmania Parasites Drive PD-L1 Expression in Mice and Human Neutrophils With Suppressor Capacity |
| title_sort |
Leishmania Parasites Drive PD-L1 Expression in Mice and Human Neutrophils With Suppressor Capacity |
| author |
Martins, Alessandra M. da Fonseca |
| author_facet |
Martins, Alessandra M. da Fonseca Gomes, Phillipe de Souza Lima Antunes, Maísa Mota Moura, Renan Garcia de Covre, Luciana P. Calôba, Carolina Rocha, Vivian Grizente Pereira, Renata M. Menezes, Gustavo Batista Gomes, Daniel Claudio Oliveira Saraiva, Elvira M. Guedes, Herbet L. de Matos |
| author_role |
author |
| author2 |
Gomes, Phillipe de Souza Lima Antunes, Maísa Mota Moura, Renan Garcia de Covre, Luciana P. Calôba, Carolina Rocha, Vivian Grizente Pereira, Renata M. Menezes, Gustavo Batista Gomes, Daniel Claudio Oliveira Saraiva, Elvira M. Guedes, Herbet L. de Matos |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Martins, Alessandra M. da Fonseca Gomes, Phillipe de Souza Lima Antunes, Maísa Mota Moura, Renan Garcia de Covre, Luciana P. Calôba, Carolina Rocha, Vivian Grizente Pereira, Renata M. Menezes, Gustavo Batista Gomes, Daniel Claudio Oliveira Saraiva, Elvira M. Guedes, Herbet L. de Matos |
| dc.subject.other.pt_BR.fl_str_mv |
PD-L1 Neutrófilos Leishmania Pele Leishmaniose cutânea humana Leishmaniose murina Hibridização Subtrativa por Supressão |
| topic |
PD-L1 Neutrófilos Leishmania Pele Leishmaniose cutânea humana Leishmaniose murina Hibridização Subtrativa por Supressão PD-L1 Neutrophils Skin Leishmania Human cutaneous leishmaniasis Murine leishmaniasis Supression Técnicas de Hibridização Subtrativa Hibridização Subtrativa por Supressão |
| dc.subject.en.pt_BR.fl_str_mv |
PD-L1 Neutrophils Skin Leishmania Human cutaneous leishmaniasis Murine leishmaniasis Supression |
| dc.subject.decs.pt_BR.fl_str_mv |
Técnicas de Hibridização Subtrativa Hibridização Subtrativa por Supressão |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Goes. Departamento de Imunologia. Laboratório de Imunobiologia das Leishmanioses. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Laborato´rio de Imunobiotecnologia. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021-06-30T14:11:48Z |
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2021-06-30T14:11:48Z |
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2021 |
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MARTINS, Alessandra M. da Fonseca et al. Leishmania parasites drive PD-L1 expression in mice and human neutrophils with suppressor capacity. Frontiers in Immunology, v. 12, Article 598943, 15 p, June 2021. |
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https://www.arca.fiocruz.br/handle/icict/47929 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2021.598943 |
| identifier_str_mv |
MARTINS, Alessandra M. da Fonseca et al. Leishmania parasites drive PD-L1 expression in mice and human neutrophils with suppressor capacity. Frontiers in Immunology, v. 12, Article 598943, 15 p, June 2021. 1664-3224 10.3389/fimmu.2021.598943 |
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