Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection

Detalhes bibliográficos
Autor(a) principal: Koishi, Andrea Cristine
Data de Publicação: 2018
Outros Autores: Suzukawa, Andréia Akemi, Zanluca, Camila, Camacho, Daria Elena, Comach, Guillermo, Santos, Claudia Nunes Duarte dos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31681
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
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spelling Koishi, Andrea CristineSuzukawa, Andréia AkemiZanluca, CamilaCamacho, Daria ElenaComach, GuillermoSantos, Claudia Nunes Duarte dos2019-02-15T14:43:57Z2019-02-15T14:43:57Z2018KOISHI, Andrea Cristine et al. Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection. PLoS Negl. Trop. Dis., v. 12, n. 3, p. 1-15, 2018.1935-2735https://www.arca.fiocruz.br/handle/icict/3168110.1371/journal.pntd.0006342porPublic Library of ScienceFlavivirusZika VirusClinical DiagnosisSeroepidemiologic StudiesSerologic TestsVirus ZikaEstudios SeroepidemiológicosPruebas SerológicasZika virusDiagnóstico ClínicoEstudos SoroepidemiológicosTestes SorológicosDevelopment and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidad de Carabobo. Instituto de Investigaciones Biomédicas. Laboratório Regional de Diagnostico e Investigación del Dengue y otras Enfermedades Virales. Maracay, Venezuela.Universidad de Carabobo. Instituto de Investigaciones Biomédicas. Laboratório Regional de Diagnostico e Investigación del Dengue y otras Enfermedades Virales. Maracay, Venezuela.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Zika virus (ZIKV) is an emerging arbovirus belonging to the genus flavivirus that comprises other important public health viruses, such as dengue (DENV) and yellow fever (YFV). In general, ZIKV infection is a self-limiting disease, however cases of Guillain-Barré syndrome and congenital brain abnormalities in newborn infants have been reported. Diagnosing ZIKV infection remains a challenge, as viral RNA detection is only applicable until a few days after the onset of symptoms. After that, serological tests must be applied, and, as expected, high cross-reactivity between ZIKV and other flavivirus serology is observed. Plaque reduction neutralization test (PRNT) is indicated to confirm positive samples for being more specific, however it is laborious intensive and time consuming, representing a major bottleneck for patient diagnosis. To overcome this limitation, we developed a high-throughput image-based fluorescent neutralization test for ZIKV infection by serological detection. Using 226 human specimens, we showed that the new test presented higher throughput than traditional PRNT, maintaining the correlation between results. Furthermore, when tested with dengue virus samples, it showed 50.53% less cross reactivity than MAC-ELISA. This fluorescent neutralization test could be used for clinical diagnosis confirmation of ZIKV infection, as well as for vaccine clinical trials and seroprevalence studies.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/31681/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALKoishiDevel.pdfKoishiDevel.pdfapplication/pdf5210592https://www.arca.fiocruz.br/bitstream/icict/31681/2/KoishiDevel.pdf8f88a42496a6df547bf14f5296936116MD52TEXTKoishiDevel.pdf.txtKoishiDevel.pdf.txtExtracted texttext/plain46406https://www.arca.fiocruz.br/bitstream/icict/31681/3/KoishiDevel.pdf.txt634a04fa36e31cfb2306fc4fd6ca7c28MD53icict/316812019-02-17 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dc.title.pt_BR.fl_str_mv Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
title Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
spellingShingle Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
Koishi, Andrea Cristine
Flavivirus
Zika Virus
Clinical Diagnosis
Seroepidemiologic Studies
Serologic Tests
Virus Zika
Estudios Seroepidemiológicos
Pruebas Serológicas
Zika virus
Diagnóstico Clínico
Estudos Soroepidemiológicos
Testes Sorológicos
title_short Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
title_full Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
title_fullStr Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
title_full_unstemmed Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
title_sort Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
author Koishi, Andrea Cristine
author_facet Koishi, Andrea Cristine
Suzukawa, Andréia Akemi
Zanluca, Camila
Camacho, Daria Elena
Comach, Guillermo
Santos, Claudia Nunes Duarte dos
author_role author
author2 Suzukawa, Andréia Akemi
Zanluca, Camila
Camacho, Daria Elena
Comach, Guillermo
Santos, Claudia Nunes Duarte dos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Koishi, Andrea Cristine
Suzukawa, Andréia Akemi
Zanluca, Camila
Camacho, Daria Elena
Comach, Guillermo
Santos, Claudia Nunes Duarte dos
dc.subject.other.pt_BR.fl_str_mv Flavivirus
topic Flavivirus
Zika Virus
Clinical Diagnosis
Seroepidemiologic Studies
Serologic Tests
Virus Zika
Estudios Seroepidemiológicos
Pruebas Serológicas
Zika virus
Diagnóstico Clínico
Estudos Soroepidemiológicos
Testes Sorológicos
dc.subject.en.pt_BR.fl_str_mv Zika Virus
Clinical Diagnosis
Seroepidemiologic Studies
Serologic Tests
dc.subject.es.pt_BR.fl_str_mv Virus Zika
Estudios Seroepidemiológicos
Pruebas Serológicas
dc.subject.decs.pt_BR.fl_str_mv Zika virus
Diagnóstico Clínico
Estudos Soroepidemiológicos
Testes Sorológicos
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-15T14:43:57Z
dc.date.available.fl_str_mv 2019-02-15T14:43:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv KOISHI, Andrea Cristine et al. Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection. PLoS Negl. Trop. Dis., v. 12, n. 3, p. 1-15, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31681
dc.identifier.issn.pt_BR.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0006342
identifier_str_mv KOISHI, Andrea Cristine et al. Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection. PLoS Negl. Trop. Dis., v. 12, n. 3, p. 1-15, 2018.
1935-2735
10.1371/journal.pntd.0006342
url https://www.arca.fiocruz.br/handle/icict/31681
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31681/1/license.txt
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