Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil

Detalhes bibliográficos
Autor(a) principal: Oliveira, Manoel Marques Evangelista
Data de Publicação: 2011
Outros Autores: Almeida-Paes, Rodrigo, Muniz, Mauro Medeiros, Gutierrez-Galhardo, Maria Clara, Zancopé-Oliveira, Rosely Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1007/s11046-011-9437-3
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33290
Resumo: 2020-05-30
id CRUZ_c1582cd0ff5c071fd74693be1a63269d
oai_identifier_str oai:www.arca.fiocruz.br:icict/33290
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Oliveira, Manoel Marques EvangelistaAlmeida-Paes, RodrigoMuniz, Mauro MedeirosGutierrez-Galhardo, Maria ClaraZancopé-Oliveira, Rosely Maria2019-05-30T13:42:57Z2019-05-30T13:42:57Z2011OLIVEIRA, Manoel Marques Evangelista et al. Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil. Mycopathologia, v. 172, p. 257-267, 2011.0301-486Xhttps://www.arca.fiocruz.br/handle/icict/3329010.1007/s11046-011-9437-31573-0832engSpringer VerlagPhenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-05-30Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Sporotrichosis has significantly increased in Brazil in the last decade, particularly in the state of Rio de Janeiro, with the occurrence of an epidemic related to zoonotic transmission from cats to humans. Recently, four new phylogenetic species were incorporated into the Sporothrix species complex based on the phenotypic and molecular characteristics, and a new species name (Sporothrix brasiliensis) was proposed for some of the Sporothrix isolates from this epidemic. This study describes the characterization of 246 isolates obtained from patients attending the Laboratory of Infectious Dermatology, IPECFIOCRUZ, between 1998 and 2008, together with one environmental sample. Two hundred and six of the isolates (83.4%) were characterized as S. brasiliensis, 15 (6.0%) as S. schenckii, and one (0.5%) as S. mexicana. Twenty-five isolates (10.1%) could not be identified according to their phenotype and were classified as Sporothrix spp. The calmodulin gene was sequenced to confirm the identity of these isolates. The molecular analysis demonstrated that 24 of the isolates were S. brasiliensis, with the remainder being a S. globosa isolate. The isolate characterized phenotypically as S. mexicana was clustered on the S. schenckii clade. The correlation between molecular data and phenotypic characteristics described in this study is fundamental to the identification of the Sporothrix complex.Sporothrix species complexSporotrichosisRio de JaneiroCalmodulin geneIdentificationTaxonomyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33290/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Oliveira_Manoel_etal_INI_2010.pdfve_Oliveira_Manoel_etal_INI_2010.pdfapplication/pdf262653https://www.arca.fiocruz.br/bitstream/icict/33290/2/ve_Oliveira_Manoel_etal_INI_2010.pdf3855a4f921df34c9bb807c75b8fb6c4cMD52TEXTve_Oliveira_Manoel_etal_INI_2010.pdf.txtve_Oliveira_Manoel_etal_INI_2010.pdf.txtExtracted texttext/plain18978https://www.arca.fiocruz.br/bitstream/icict/33290/3/ve_Oliveira_Manoel_etal_INI_2010.pdf.txtca2222f018fa6459c7a6ebba090c86e4MD53icict/332902021-03-24 16:32:32.571oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:32Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil
title Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil
spellingShingle Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil
Oliveira, Manoel Marques Evangelista
Sporothrix species complex
Sporotrichosis
Rio de Janeiro
Calmodulin gene
Identification
Taxonomy
title_short Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil
title_full Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil
title_fullStr Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil
title_full_unstemmed Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil
title_sort Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil
author Oliveira, Manoel Marques Evangelista
author_facet Oliveira, Manoel Marques Evangelista
Almeida-Paes, Rodrigo
Muniz, Mauro Medeiros
Gutierrez-Galhardo, Maria Clara
Zancopé-Oliveira, Rosely Maria
author_role author
author2 Almeida-Paes, Rodrigo
Muniz, Mauro Medeiros
Gutierrez-Galhardo, Maria Clara
Zancopé-Oliveira, Rosely Maria
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Manoel Marques Evangelista
Almeida-Paes, Rodrigo
Muniz, Mauro Medeiros
Gutierrez-Galhardo, Maria Clara
Zancopé-Oliveira, Rosely Maria
dc.subject.en.pt_BR.fl_str_mv Sporothrix species complex
Sporotrichosis
Rio de Janeiro
Calmodulin gene
Identification
Taxonomy
topic Sporothrix species complex
Sporotrichosis
Rio de Janeiro
Calmodulin gene
Identification
Taxonomy
description 2020-05-30
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2019-05-30T13:42:57Z
dc.date.available.fl_str_mv 2019-05-30T13:42:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Manoel Marques Evangelista et al. Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil. Mycopathologia, v. 172, p. 257-267, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33290
dc.identifier.issn.pt_BR.fl_str_mv 0301-486X
dc.identifier.doi.none.fl_str_mv 10.1007/s11046-011-9437-3
dc.identifier.eissn.none.fl_str_mv 1573-0832
identifier_str_mv OLIVEIRA, Manoel Marques Evangelista et al. Phenotypic and molecular identification of Sporothrix isolates from an epidemic area of sporotrichosis in Brazil. Mycopathologia, v. 172, p. 257-267, 2011.
0301-486X
10.1007/s11046-011-9437-3
1573-0832
url https://www.arca.fiocruz.br/handle/icict/33290
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33290/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/33290/2/ve_Oliveira_Manoel_etal_INI_2010.pdf
https://www.arca.fiocruz.br/bitstream/icict/33290/3/ve_Oliveira_Manoel_etal_INI_2010.pdf.txt
bitstream.checksum.fl_str_mv 8a4605be74aa9ea9d79846c1fba20a33
3855a4f921df34c9bb807c75b8fb6c4c
ca2222f018fa6459c7a6ebba090c86e4
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1822791934031167488

Registros relacionados