A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines

Detalhes bibliográficos
Autor(a) principal: Carels, Nicolas
Data de Publicação: 2017
Outros Autores: Gumiel, Marcial, Mota, Fabio Faria da, Moreira, Carlos José de Carvalho, Azambuja, Patricia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24758
Resumo: Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Laboratório de Modelagem de Sistemas Biológicos. Rio de Janeiro, RJ, Brasil.
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spelling Carels, NicolasGumiel, MarcialMota, Fabio Faria daMoreira, Carlos José de CarvalhoAzambuja, Patricia2018-02-08T11:56:48Z2018-02-08T11:56:48Z2017CARELS, Nicolas; et al. A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines. Bioinformatics and Biology Insights, v.11, p.1-19, 2017.https://www.arca.fiocruz.br/handle/icict/2475810.1177/11779322177334221177-9322engLibertas AcademicaNicho ecológicoMicrobiota BacterianaIntestino médioAnálise MetagenômicaTrato Digestivo de TriatomíneosDigestive Tract of Triatominesecological nichemidgutMetagenomic AnalysisBacterial MicrobiotaA Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatominesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Laboratório de Modelagem de Sistemas Biológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Entomologia Molecular. Departamento de Entomologia Molecular. Rio de Janeiro, RJ, Brasil.The digestive tract of triatomines (DTT) is an ecological niche favored by microbiota whose enzymatic profile is adapted to the specific substrate availability in this medium. This report describes the molecular enzymatic properties that promote bacterial prominence in the DTT. The microbiota composition was assessed previously based on 16S ribosomal DNA, and whole sequenced genomes of bacteria from the same genera were used to calculate the GC level of rare and prominent bacterial species in the DTT. The enzymatic reactions encoded by coding sequences of both rare and common bacterial species were then compared and revealed key functions explaining why some genera outcompete others in the DTT. Representativeness of DTT microbiota was investigated by shotgun sequencing of DNA extracted from bacteria grown in liquid Luria-Bertani broth (LB) medium. Results showed that GC-rich bacteria outcompete GC-poor bacteria and are the dominant components of the DTT microbiota. In addition, oxidoreductases are the main enzymatic components of these bacteria. In particular, nitrate reductases (anaerobic respiration), oxygenases (catabolism of complex substrates), acetate-CoA ligase (tricarboxylic acid cycle and energy metabolism), and kinase (signaling pathway) were the major enzymatic determinants present together with a large group of minor enzymes including hydrogenases involved in energy and amino acid metabolism. In conclusion, despite their slower growth in liquid LB medium, bacteria from GC-rich genera outcompete the GC-poor bacteria because their specific enzymatic abilities impart a selective advantage in the DTT.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24758/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmarcial_gumiel_etal_IOC_2017.pdfmarcial_gumiel_etal_IOC_2017.pdfapplication/pdf691965https://www.arca.fiocruz.br/bitstream/icict/24758/2/marcial_gumiel_etal_IOC_2017.pdfc4fac12518afd700cc48fbc6c640f94bMD52TEXTmarcial_gumiel_etal_IOC_2017.pdf.txtmarcial_gumiel_etal_IOC_2017.pdf.txtExtracted texttext/plain87652https://www.arca.fiocruz.br/bitstream/icict/24758/3/marcial_gumiel_etal_IOC_2017.pdf.txtd17737293c6b2390f3100f669fbf1bd1MD53icict/247582018-08-15 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dc.title.pt_BR.fl_str_mv A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines
title A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines
spellingShingle A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines
Carels, Nicolas
Nicho ecológico
Microbiota Bacteriana
Intestino médio
Análise Metagenômica
Trato Digestivo de Triatomíneos
Digestive Tract of Triatomines
ecological niche
midgut
Metagenomic Analysis
Bacterial Microbiota
title_short A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines
title_full A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines
title_fullStr A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines
title_full_unstemmed A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines
title_sort A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines
author Carels, Nicolas
author_facet Carels, Nicolas
Gumiel, Marcial
Mota, Fabio Faria da
Moreira, Carlos José de Carvalho
Azambuja, Patricia
author_role author
author2 Gumiel, Marcial
Mota, Fabio Faria da
Moreira, Carlos José de Carvalho
Azambuja, Patricia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carels, Nicolas
Gumiel, Marcial
Mota, Fabio Faria da
Moreira, Carlos José de Carvalho
Azambuja, Patricia
dc.subject.other.pt_BR.fl_str_mv Nicho ecológico
Microbiota Bacteriana
Intestino médio
Análise Metagenômica
Trato Digestivo de Triatomíneos
topic Nicho ecológico
Microbiota Bacteriana
Intestino médio
Análise Metagenômica
Trato Digestivo de Triatomíneos
Digestive Tract of Triatomines
ecological niche
midgut
Metagenomic Analysis
Bacterial Microbiota
dc.subject.en.pt_BR.fl_str_mv Digestive Tract of Triatomines
ecological niche
midgut
Metagenomic Analysis
Bacterial Microbiota
description Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Laboratório de Modelagem de Sistemas Biológicos. Rio de Janeiro, RJ, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-08T11:56:48Z
dc.date.available.fl_str_mv 2018-02-08T11:56:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARELS, Nicolas; et al. A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines. Bioinformatics and Biology Insights, v.11, p.1-19, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24758
dc.identifier.doi.none.fl_str_mv 10.1177/1177932217733422
dc.identifier.eissn.none.fl_str_mv 1177-9322
identifier_str_mv CARELS, Nicolas; et al. A Metagenomic Analysis of Bacterial Microbiota in the Digestive Tract of Triatomines. Bioinformatics and Biology Insights, v.11, p.1-19, 2017.
10.1177/1177932217733422
1177-9322
url https://www.arca.fiocruz.br/handle/icict/24758
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Libertas Academica
publisher.none.fl_str_mv Libertas Academica
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/24758/2/marcial_gumiel_etal_IOC_2017.pdf
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