Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice

Pereira, V. R. A.; Lorena, Virginia Maria Barros de; Silva, Ana Paula Galvão da; Coutinho, Eridan de Medeiros; Silva, Emilson Domingos da; Ferreira, Antonio Gomes Pinto; Miranda, P.; Krieger, Marco Aurélio; Goldenberg, Samuel; Soares, Milena Botelho Pereira; Oliveira, R Correa; Gomes, Yara de Miranda

Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice

Detalhes bibliográficos
Autor(a) principal:	Pereira, V. R. A.
Data de Publicação:	2004
Outros Autores:	Lorena, Virginia Maria Barros de, Silva, Ana Paula Galvão da, Coutinho, Eridan de Medeiros, Silva, Emilson Domingos da, Ferreira, Antonio Gomes Pinto, Miranda, P., Krieger, Marco Aurélio, Goldenberg, Samuel, Soares, Milena Botelho Pereira, Oliveira, R Correa, Gomes, Yara de Miranda
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/9949
Resumo:	Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil.

Metadados do item

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spelling	Pereira, V. R. A.Lorena, Virginia Maria Barros deSilva, Ana Paula Galvão daCoutinho, Eridan de MedeirosSilva, Emilson Domingos daFerreira, Antonio Gomes PintoMiranda, P.Krieger, Marco AurélioGoldenberg, SamuelSoares, Milena Botelho PereiraOliveira, R CorreaGomes, Yara de Miranda2015-04-10T14:27:34Z2015-04-10T14:27:34Z2004PEREIRA, V. R. A. et al. Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice. Parasitology, v. 129, n. Pt 5, p. 563-570, 2004.0031-1820https://www.arca.fiocruz.br/handle/icict/994910.1017/S0031182004006043engCambridge University PressImmunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil.Universidade Federal de Pernambuco. Recife, PE, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal de Pernambuco. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil.In previous studies, we demonstrated that CRA and FRA recombinant proteins, used for diagnosis of Chagas' disease, elicited a humoral immune response in susceptible and resistant mice. To understand better the immune response to these proteins, we have evaluated, the cellular immune response in CRA- and in FRA-immunized BALB/c and C57BL/6 mice. A specific cellular lymphoproliferative response was observed in both strains of mice. Spleen cell cultures mainly from CRA-immunized C57BL/6 and FRA-immunized BALB/c mice produced high levels of IFN-y, indicating the induction of a Type 1 immune response. Regarding the T cell subsets, CD4+ T cells were the major source of IFN-y in CRA- and FRA-immunized mice. These results suggest that CRA and FRA are important immunogens in inducing a Type 1 immune response and that they may be considered as potential vaccine antigens.Recombinant proteinsImmune cellular responseTrypanosoma cruziAntígenos de Protozoários/imunologiaImunidade CelularProteínas de Protozoários/imunologiaTrypanosoma cruzi/imunologiaAnimaisAntígenos de Protozoários/administração & dosagemCitocinas/biossínteseCitometria de FluxoImunidade Celular/imunologiaImunizaçãoAtivação Linfocitária/imunologiaMasculinoCamundongosCamundongos Endogâmicos BALB Cinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/9949/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALPereira V R A Immunization with....pdfPereira V R A Immunization with....pdfapplication/pdf111858https://www.arca.fiocruz.br/bitstream/icict/9949/2/Pereira%20V%20R%20A%20Immunization%20with....pdf4793453ce315eeed8d73c164a1323364MD52TEXTPereira V R A Immunization with....pdf.txtPereira V R A Immunization with....pdf.txtExtracted texttext/plain37518https://www.arca.fiocruz.br/bitstream/icict/9949/3/Pereira%20V%20R%20A%20Immunization%20with....pdf.txtb5511b545150c436b8cd00283e8dbc26MD53icict/99492023-03-15 14:34:19.592oai:www.arca.fiocruz.br:icict/9949Q0VTU8ODTyBOw4NPLUVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcyBhdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKQ0VERSBlIFRSQU5TRkVSRSwgdG90YWwgZSBncmF0dWl0YW1lbnRlLCDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVosIGVtIGNhcsOhdGVyIHBlcm1hbmVudGUsIGlycmV2b2fDoXZlbCBlIE7Dg08gRVhDTFVTSVZPLCAKdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08gQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcyAKZGUgdm96IGUgaW1hZ2VtIHZpbmN1bGFkb3Mgw6AgT0JSQSwgZHVyYW50ZSB0b2RvIG8gcHJhem8gZGUgZHVyYcOnw6NvIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgZW0gcXVhbHF1ZXIgaWRpb21hIGUgZW0gdG9kb3Mgb3MgcGHDrXNlczsKICAgICAgICAKQUNFSVRBIHF1ZSBhIGNlc3PDo28gdG90YWwgbsOjbyBleGNsdXNpdmEsIHBlcm1hbmVudGUgZSBpcnJldm9nw6F2ZWwgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhdHJpbW9uaWFpcyBuw6NvIGNvbWVyY2lhaXMgZGUgdXRpbGl6YcOnw6NvIApkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwgb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciAKbWVpbyBvdSB2ZcOtY3VsbywgaW5jbHVzaXZlIGVtIFJlcG9zaXTDs3Jpb3MgRGlnaXRhaXMsIGJlbSBjb21vIG9zIGRpcmVpdG9zIGRlIHJlcHJvZHXDp8OjbywgZXhpYmnDp8OjbywgZXhlY3XDp8OjbywgZGVjbGFtYcOnw6NvLCBleHBvc2nDp8OjbywgCmFycXVpdmFtZW50bywgaW5jbHVzw6NvIGVtIGJhbmNvIGRlIGRhZG9zLCBwcmVzZXJ2YcOnw6NvLCBkaWZ1c8OjbywgZGlzdHJpYnVpw6fDo28sIGRpdnVsZ2HDp8OjbywgZW1wcsOpc3RpbW8sIHRyYWR1w6fDo28sIGluY2x1c8OjbyBlbSBub3ZhcyAKb2JyYXMgb3UgY29sZXTDom5lYXMsIHJldXRpbGl6YcOnw6NvLCBlZGnDp8OjbywgcHJvZHXDp8OjbyBkZSBtYXRlcmlhbCBkaWTDoXRpY28gZSBjdXJzb3Mgb3UgcXVhbHF1ZXIgZm9ybWEgZGUgdXRpbGl6YcOnw6NvIG7Do28gY29tZXJjaWFsOwogICAgICAgIApSRUNPTkhFQ0UgcXVlIGEgY2Vzc8OjbyBhcXVpIGVzcGVjaWZpY2FkYSBjb25jZWRlIMOgIEZJT0NSVVogLSBGVU5EQcOHw4NPIE9TV0FMRE8gQ1JVWiBvIGRpcmVpdG8gZGUgYXV0b3JpemFyIHF1YWxxdWVyIHBlc3NvYSDigJMgZsOtc2ljYSAKb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdSBlc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlciAKZmluYWxpZGFkZXMgbsOjbyBjb21lcmNpYWlzOwogICAgICAgIApERUNMQVJBIHF1ZSBhIG9icmEgw6kgY3JpYcOnw6NvIG9yaWdpbmFsIGUgcXVlIMOpIG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgYXF1aSBjZWRpZG9zIGUgYXV0b3JpemFkb3MsIHJlc3BvbnNhYmlsaXphbmRvLXNlIGludGVncmFsbWVudGUgCnBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwgaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIAppbmRlbml6YXIgdGVyY2Vpcm9zIHBvciBkYW5vcywgYmVtIGNvbW8gaW5kZW5pemFyIGUgcmVzc2FyY2lyIGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIGRlIGV2ZW50dWFpcyBkZXNwZXNhcyBxdWUgdmllcmVtIGEgCnN1cG9ydGFyLCBlbSByYXrDo28gZGUgcXVhbHF1ZXIgb2ZlbnNhIGEgZGlyZWl0b3MgYXV0b3JhaXMgb3UgZGlyZWl0b3MgZGUgdm96IG91IGltYWdlbSwgcHJpbmNpcGFsbWVudGUgbm8gcXVlIGRpeiByZXNwZWl0byBhIHBsw6FnaW8gCmUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CiAgICAgICAgCkFGSVJNQSBxdWUgY29uaGVjZSBhIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgSW5zdGl0dWnDp8OjbyBlIGFzIGRpcmV0cml6ZXMgcGFyYSBvIGZ1bmNpb25hbWVudG8gZG8gcmVwb3NpdMOzcmlvIAppbnN0aXR1Y2lvbmFsIEFSQ0EKRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice
title	Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice
spellingShingle	Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice Pereira, V. R. A. Recombinant proteins Immune cellular response Trypanosoma cruzi Antígenos de Protozoários/imunologia Imunidade Celular Proteínas de Protozoários/imunologia Trypanosoma cruzi/imunologia Animais Antígenos de Protozoários/administração & dosagem Citocinas/biossíntese Citometria de Fluxo Imunidade Celular/imunologia Imunização Ativação Linfocitária/imunologia Masculino Camundongos Camundongos Endogâmicos BALB C
title_short	Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice
title_full	Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice
title_fullStr	Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice
title_full_unstemmed	Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice
title_sort	Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice
author	Pereira, V. R. A.
author_facet	Pereira, V. R. A. Lorena, Virginia Maria Barros de Silva, Ana Paula Galvão da Coutinho, Eridan de Medeiros Silva, Emilson Domingos da Ferreira, Antonio Gomes Pinto Miranda, P. Krieger, Marco Aurélio Goldenberg, Samuel Soares, Milena Botelho Pereira Oliveira, R Correa Gomes, Yara de Miranda
author_role	author
author2	Lorena, Virginia Maria Barros de Silva, Ana Paula Galvão da Coutinho, Eridan de Medeiros Silva, Emilson Domingos da Ferreira, Antonio Gomes Pinto Miranda, P. Krieger, Marco Aurélio Goldenberg, Samuel Soares, Milena Botelho Pereira Oliveira, R Correa Gomes, Yara de Miranda
author2_role	author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Pereira, V. R. A. Lorena, Virginia Maria Barros de Silva, Ana Paula Galvão da Coutinho, Eridan de Medeiros Silva, Emilson Domingos da Ferreira, Antonio Gomes Pinto Miranda, P. Krieger, Marco Aurélio Goldenberg, Samuel Soares, Milena Botelho Pereira Oliveira, R Correa Gomes, Yara de Miranda
dc.subject.en.pt_BR.fl_str_mv	Recombinant proteins Immune cellular response Trypanosoma cruzi
topic	Recombinant proteins Immune cellular response Trypanosoma cruzi Antígenos de Protozoários/imunologia Imunidade Celular Proteínas de Protozoários/imunologia Trypanosoma cruzi/imunologia Animais Antígenos de Protozoários/administração & dosagem Citocinas/biossíntese Citometria de Fluxo Imunidade Celular/imunologia Imunização Ativação Linfocitária/imunologia Masculino Camundongos Camundongos Endogâmicos BALB C
dc.subject.decs.pt_BR.fl_str_mv	Antígenos de Protozoários/imunologia Imunidade Celular Proteínas de Protozoários/imunologia Trypanosoma cruzi/imunologia Animais Antígenos de Protozoários/administração & dosagem Citocinas/biossíntese Citometria de Fluxo Imunidade Celular/imunologia Imunização Ativação Linfocitária/imunologia Masculino Camundongos Camundongos Endogâmicos BALB C
description	Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil.
publishDate	2004
dc.date.issued.fl_str_mv	2004
dc.date.accessioned.fl_str_mv	2015-04-10T14:27:34Z
dc.date.available.fl_str_mv	2015-04-10T14:27:34Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	PEREIRA, V. R. A. et al. Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice. Parasitology, v. 129, n. Pt 5, p. 563-570, 2004.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/9949
dc.identifier.issn.none.fl_str_mv	0031-1820
dc.identifier.doi.none.fl_str_mv	10.1017/S0031182004006043
identifier_str_mv	PEREIRA, V. R. A. et al. Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice. Parasitology, v. 129, n. Pt 5, p. 563-570, 2004. 0031-1820 10.1017/S0031182004006043
url	https://www.arca.fiocruz.br/handle/icict/9949
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Cambridge University Press
publisher.none.fl_str_mv	Cambridge University Press
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice

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