Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals.

Detalhes bibliográficos
Autor(a) principal: Miranda, Aline Cristina Andrade Mota
Data de Publicação: 2013
Outros Autores: Barreto, Fernanda Khouri, Baptista, Everton, Vale, Lourdes Farre, Cunha, Joana Paixão Monteiro, Castro Filho, Bernardo Galvão, Alcantara, Luiz Carlos Júnior
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1089/aid.2013.0015
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7789
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil /Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil
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spelling Miranda, Aline Cristina Andrade MotaBarreto, Fernanda KhouriBaptista, EvertonVale, Lourdes FarreCunha, Joana Paixão MonteiroCastro Filho, Bernardo GalvãoAlcantara, Luiz Carlos Júnior2014-06-04T12:54:34Z2014-06-04T12:54:34Z2013MOTA-MIRANDA, A.C. A. et al. Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals. AIDS Research and Human Retroviruses, v. 29, n. 10, p. 1370-1372, 2013.1931-8405https://www.arca.fiocruz.br/handle/icict/778910.1089/aid.2013.0015engMary Ann Liebert, IncMolecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil /Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil /Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilHuman T cell leukemia virus type 1 (HTLV-1) is associated with a neurological syndrome named tropical spastic paraparesis/HTLV-associated myelopathy (TSP/HAM) and the disease progression involves viral factors. The gp21 glycoprotein is involved in envelope trafficking and membrane targeting while the bZIP protein is indispensable for cell growth and proliferation. This study aimed to assess the molecular diversity of gp21 and HBZ proteins in TSP/HAM and healthy carriers. DNA samples from HTLV-1-infected individuals were submitted to PCR and sequencing, and the molecular analyses were performed using bioinformatics tools. From eight gp21-analyzed sequences one amino acid change (Y477H) was associated with the switch of a helix to coil structure at secondary structure prediction. From 10 HBZ analyzed sequences, two amino acid changes were identified (S9P and T95I) at the activation domain. One mutation (R112C) located at the nuclear localization signal was present in 66.7% and 25% of healthy carriers (HC) and TSP/HAM groups, respectively. This is the first report of mutations in the HBZ region. These polymorphisms might be important for viral fitness.Fatores de Transcrição de Zíper de Leucina Básica/genéticaVariação GenéticaInfecções por HTLV-I/virologiaVírus 1 Linfotrópico T Humano/genéticaProteínas Virais/genéticaProdutos do Gene env do Vírus da Imunodeficiência Humana/genéticaAdultoIdosoFemininoVírus 1 Linfotrópico T Humano/isolamento & purificaçãoHumanosMasculinoMeia-IdadeDados de Sequência MolecularProteínas Mutantes/genéticaMutação PuntualRNA Viral/genéticaAnálise de Sequência de DNAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALMota-Miranda ACA Molecular study of HBZ....pdfMota-Miranda ACA Molecular study of HBZ....pdfapplication/pdf136604https://www.arca.fiocruz.br/bitstream/icict/7789/1/Mota-Miranda%20ACA%20Molecular%20study%20of%20HBZ....pdf7ba88357a68622e23fedaa32936ce20eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7789/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTMota-Miranda ACA Molecular study of HBZ....pdf.txtMota-Miranda ACA Molecular study of HBZ....pdf.txtExtracted texttext/plain14842https://www.arca.fiocruz.br/bitstream/icict/7789/5/Mota-Miranda%20ACA%20Molecular%20study%20of%20HBZ....pdf.txt3a1f55afe40babe0c3f1be420755fa4cMD55THUMBNAILMota-Miranda ACA Molecular study of HBZ....pdf.jpgMota-Miranda ACA Molecular study of HBZ....pdf.jpgGenerated Thumbnailimage/jpeg2187https://www.arca.fiocruz.br/bitstream/icict/7789/4/Mota-Miranda%20ACA%20Molecular%20study%20of%20HBZ....pdf.jpg8e10f2ea30672067c822277a9ee34e37MD54icict/77892023-03-15 14:33:19.547oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals.
title Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals.
spellingShingle Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals.
Miranda, Aline Cristina Andrade Mota
Fatores de Transcrição de Zíper de Leucina Básica/genética
Variação Genética
Infecções por HTLV-I/virologia
Vírus 1 Linfotrópico T Humano/genética
Proteínas Virais/genética
Produtos do Gene env do Vírus da Imunodeficiência Humana/genética
Adulto
Idoso
Feminino
Vírus 1 Linfotrópico T Humano/isolamento & purificação
Humanos
Masculino
Meia-Idade
Dados de Sequência Molecular
Proteínas Mutantes/genética
Mutação Puntual
RNA Viral/genética
Análise de Sequência de DNA
title_short Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals.
title_full Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals.
title_fullStr Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals.
title_full_unstemmed Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals.
title_sort Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals.
author Miranda, Aline Cristina Andrade Mota
author_facet Miranda, Aline Cristina Andrade Mota
Barreto, Fernanda Khouri
Baptista, Everton
Vale, Lourdes Farre
Cunha, Joana Paixão Monteiro
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
author_role author
author2 Barreto, Fernanda Khouri
Baptista, Everton
Vale, Lourdes Farre
Cunha, Joana Paixão Monteiro
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Miranda, Aline Cristina Andrade Mota
Barreto, Fernanda Khouri
Baptista, Everton
Vale, Lourdes Farre
Cunha, Joana Paixão Monteiro
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
dc.subject.decs.pt_BR.fl_str_mv Fatores de Transcrição de Zíper de Leucina Básica/genética
Variação Genética
Infecções por HTLV-I/virologia
Vírus 1 Linfotrópico T Humano/genética
Proteínas Virais/genética
Produtos do Gene env do Vírus da Imunodeficiência Humana/genética
Adulto
Idoso
Feminino
Vírus 1 Linfotrópico T Humano/isolamento & purificação
Humanos
Masculino
Meia-Idade
Dados de Sequência Molecular
Proteínas Mutantes/genética
Mutação Puntual
RNA Viral/genética
Análise de Sequência de DNA
topic Fatores de Transcrição de Zíper de Leucina Básica/genética
Variação Genética
Infecções por HTLV-I/virologia
Vírus 1 Linfotrópico T Humano/genética
Proteínas Virais/genética
Produtos do Gene env do Vírus da Imunodeficiência Humana/genética
Adulto
Idoso
Feminino
Vírus 1 Linfotrópico T Humano/isolamento & purificação
Humanos
Masculino
Meia-Idade
Dados de Sequência Molecular
Proteínas Mutantes/genética
Mutação Puntual
RNA Viral/genética
Análise de Sequência de DNA
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil /Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-06-04T12:54:34Z
dc.date.available.fl_str_mv 2014-06-04T12:54:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOTA-MIRANDA, A.C. A. et al. Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals. AIDS Research and Human Retroviruses, v. 29, n. 10, p. 1370-1372, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7789
dc.identifier.issn.none.fl_str_mv 1931-8405
dc.identifier.doi.none.fl_str_mv 10.1089/aid.2013.0015
identifier_str_mv MOTA-MIRANDA, A.C. A. et al. Molecular study of HBZ and gp21 human T cell leukemia virus type 1 proteins isolated from different clinical profile infected individuals. AIDS Research and Human Retroviruses, v. 29, n. 10, p. 1370-1372, 2013.
1931-8405
10.1089/aid.2013.0015
url https://www.arca.fiocruz.br/handle/icict/7789
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Mary Ann Liebert, Inc
publisher.none.fl_str_mv Mary Ann Liebert, Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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