Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus

Detalhes bibliográficos
Autor(a) principal: Feder, D.
Data de Publicação: 1999
Outros Autores: Gomes, S. A. O., Garcia, Eloi S., Azambuja, Patricia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/S0074-02761999000600011
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44032
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Feder, D.Gomes, S. A. O.Garcia, Eloi S.Azambuja, Patricia2020-10-17T19:00:56Z2020-10-17T19:00:56Z1999FEDER, D. et al. Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus, Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 94, n. 6, p. 771-777, Nov./Dec. 1999.0074-0276https://www.arca.fiocruz.br/handle/icict/4403210.1590/S0074-027619990006000111678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Rhodnius prolixusTrypanosoma rangeliCorpo gordoMetaloproteaseHaemolymphFat bodyHaemolymphRhodnius prolixusMetalloproteaseTrypanosoma rangeliMetalloproteases in Trypanosoma rangeli-infected Rhodnius prolixusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Protease activities in the haemolymph and fat body in a bloodsucking insect, Rhodnius prolixus, infected with Trypanosoma rangeli, were investigated. After SDS-polyacrylamide gel electrophoresis containing gelatin as substrate, analysis of zymograms performed on samples of different tissues of controls and insects inoculated or orally infected with short or long epimastigotes of T. rangeli, demonstrated distinct patterns of protease activities: (i) proteases were detected in the haemolymph of insects which were fed on, or inoculated with, short epimastigotes of T. rangeli (39 kDa and 33 kDa, respectively), but they were not observed in the fat body taken from these insects; (ii) protease was also presented in the fat bodies derived from naive insects or controls inoculated with sterile phosphatesaline buffer (49 kDa), but it was not detected in the haemolymph of these insects; (iii) no protease activity was observed in both haemolymph and fat bodies taken from insects inoculated with, or fed on, long epimastigotes of T. rangeli. Furthermore, in short epimastigotes of T. rangeli extracts, three bands of the protease activities with apparent molecular weights of 297, 198 and 95 kDa were detected while long epimastigotes preparation presented only two bands of protease activities with molecular weights of 297 and 198 kDa. The proteases from the insect infected with T. rangeli and controls belong to the class of either metalloproteases or metal-activated enzymes since they are inhibited by 1,10- phenanthroline. The significance of these proteases in the insects infected with short epimastigotes of T. rangeli is discussed in relation to the success of the establishment of infection of these parasites in its vector, R. prolixus.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44032/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDFeder_EloiSGarcia_etal_IOC_1999.pdfDFeder_EloiSGarcia_etal_IOC_1999.pdfapplication/pdf82782https://www.arca.fiocruz.br/bitstream/icict/44032/2/DFeder_EloiSGarcia_etal_IOC_1999.pdf6cbed37819bf26020e39b77e1e45ec25MD52TEXTDFeder_EloiSGarcia_etal_IOC_1999.pdf.txtDFeder_EloiSGarcia_etal_IOC_1999.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus
title Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus
spellingShingle Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus
Feder, D.
Rhodnius prolixus
Trypanosoma rangeli
Corpo gordo
Metaloprotease
Haemolymph
Fat body
Haemolymph
Rhodnius prolixus
Metalloprotease
Trypanosoma rangeli
title_short Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus
title_full Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus
title_fullStr Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus
title_full_unstemmed Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus
title_sort Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus
author Feder, D.
author_facet Feder, D.
Gomes, S. A. O.
Garcia, Eloi S.
Azambuja, Patricia
author_role author
author2 Gomes, S. A. O.
Garcia, Eloi S.
Azambuja, Patricia
author2_role author
author
author
dc.contributor.author.fl_str_mv Feder, D.
Gomes, S. A. O.
Garcia, Eloi S.
Azambuja, Patricia
dc.subject.other.pt_BR.fl_str_mv Rhodnius prolixus
Trypanosoma rangeli
Corpo gordo
Metaloprotease
Haemolymph
topic Rhodnius prolixus
Trypanosoma rangeli
Corpo gordo
Metaloprotease
Haemolymph
Fat body
Haemolymph
Rhodnius prolixus
Metalloprotease
Trypanosoma rangeli
dc.subject.en.pt_BR.fl_str_mv Fat body
Haemolymph
Rhodnius prolixus
Metalloprotease
Trypanosoma rangeli
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 1999
dc.date.issued.fl_str_mv 1999
dc.date.accessioned.fl_str_mv 2020-10-17T19:00:56Z
dc.date.available.fl_str_mv 2020-10-17T19:00:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FEDER, D. et al. Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus, Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 94, n. 6, p. 771-777, Nov./Dec. 1999.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44032
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761999000600011
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv FEDER, D. et al. Metalloproteases in Trypanosoma rangeli-infected Rhodnius prolixus, Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 94, n. 6, p. 771-777, Nov./Dec. 1999.
0074-0276
10.1590/S0074-02761999000600011
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44032
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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