The prophenoloxidase system and in vitro interaction of Trypanosoma rangeli with Rhodnius prolixus and Triatoma infestans haemolymph
Autor(a) principal: | |
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Data de Publicação: | 1991 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1365-3024.1991.tb00551.x http://hdl.handle.net/11449/219147 |
Resumo: | Summary The presence of the prophenoloxidase (proPO) system in the haemolymph of Rhodnius protixus and Triatoma infestans and the role played by Trypanosoma rangeli in the in vitro activation of proPO were tested. Both R. prolixus and T. infestans whole blood preparations showed a very active ProPO system. The proPO cascade of the two insect species were differentially activated by microbial‐derived extracts: laminarin was a better activator of T. infestans haemolymph than of R. prolixus blood, and lipopolysaccharides from Shigella flexneri or Pseudomonas aeroginasa caused significant proPO activation of T. infestans haemolymph but not of R. prolixus preparations. For the two insect species, neither T. rangeli from culture nor parasite lysates were able to trigger proPO activation. The presence of the parasite in R. prolixus haemolymph/laminarin assays, however, significantly reduced the level of proPO activation to that of spontaneous activating controls. The immobilization of T. rangeli in vitro in haemolymph preparations occurred in both insect species and was dependent on the proPO activation intensity Our results suggest that the susceptibility of R. prolixus to T. rangeli haemocoel infection may be explained, at least in part, by the suppression of the insect immune defence system i.e., inhibition of proPO in the presence of this protozoan parasite. Copyright © 1991, Wiley Blackwell. All rights reserved |
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The prophenoloxidase system and in vitro interaction of Trypanosoma rangeli with Rhodnius prolixus and Triatoma infestans haemolymphprophenoloxidase. haemolymph. insect immunityRhodnius prolixus. Trypanosoma rangeliTriatoma infestansSummary The presence of the prophenoloxidase (proPO) system in the haemolymph of Rhodnius protixus and Triatoma infestans and the role played by Trypanosoma rangeli in the in vitro activation of proPO were tested. Both R. prolixus and T. infestans whole blood preparations showed a very active ProPO system. The proPO cascade of the two insect species were differentially activated by microbial‐derived extracts: laminarin was a better activator of T. infestans haemolymph than of R. prolixus blood, and lipopolysaccharides from Shigella flexneri or Pseudomonas aeroginasa caused significant proPO activation of T. infestans haemolymph but not of R. prolixus preparations. For the two insect species, neither T. rangeli from culture nor parasite lysates were able to trigger proPO activation. The presence of the parasite in R. prolixus haemolymph/laminarin assays, however, significantly reduced the level of proPO activation to that of spontaneous activating controls. The immobilization of T. rangeli in vitro in haemolymph preparations occurred in both insect species and was dependent on the proPO activation intensity Our results suggest that the susceptibility of R. prolixus to T. rangeli haemocoel infection may be explained, at least in part, by the suppression of the insect immune defence system i.e., inhibition of proPO in the presence of this protozoan parasite. Copyright © 1991, Wiley Blackwell. All rights reservedInstitute de Biociências UNESP Campus de Botucatu, Botucalu, São Paulo, 18600Biomedkal & Physiological Research Group School of Biological Sciences. University College of SwanseaInstitute de Biociências UNESP Campus de Botucatu, Botucalu, São Paulo, 18600Universidade Estadual Paulista (UNESP)University College of SwanseaGREGÓRIO, ELISA A. [UNESP]A.RATCLIFFE, NORMAN2022-04-28T18:54:00Z2022-04-28T18:54:00Z1991-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article551-564http://dx.doi.org/10.1111/j.1365-3024.1991.tb00551.xParasite Immunology, v. 13, n. 5, p. 551-564, 1991.1365-30240141-9838http://hdl.handle.net/11449/21914710.1111/j.1365-3024.1991.tb00551.x2-s2.0-0025913319Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasite Immunologyinfo:eu-repo/semantics/openAccess2022-04-28T18:54:00Zoai:repositorio.unesp.br:11449/219147Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:27:17.343756Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The prophenoloxidase system and in vitro interaction of Trypanosoma rangeli with Rhodnius prolixus and Triatoma infestans haemolymph |
title |
The prophenoloxidase system and in vitro interaction of Trypanosoma rangeli with Rhodnius prolixus and Triatoma infestans haemolymph |
spellingShingle |
The prophenoloxidase system and in vitro interaction of Trypanosoma rangeli with Rhodnius prolixus and Triatoma infestans haemolymph GREGÓRIO, ELISA A. [UNESP] prophenoloxidase. haemolymph. insect immunity Rhodnius prolixus. Trypanosoma rangeli Triatoma infestans |
title_short |
The prophenoloxidase system and in vitro interaction of Trypanosoma rangeli with Rhodnius prolixus and Triatoma infestans haemolymph |
title_full |
The prophenoloxidase system and in vitro interaction of Trypanosoma rangeli with Rhodnius prolixus and Triatoma infestans haemolymph |
title_fullStr |
The prophenoloxidase system and in vitro interaction of Trypanosoma rangeli with Rhodnius prolixus and Triatoma infestans haemolymph |
title_full_unstemmed |
The prophenoloxidase system and in vitro interaction of Trypanosoma rangeli with Rhodnius prolixus and Triatoma infestans haemolymph |
title_sort |
The prophenoloxidase system and in vitro interaction of Trypanosoma rangeli with Rhodnius prolixus and Triatoma infestans haemolymph |
author |
GREGÓRIO, ELISA A. [UNESP] |
author_facet |
GREGÓRIO, ELISA A. [UNESP] A.RATCLIFFE, NORMAN |
author_role |
author |
author2 |
A.RATCLIFFE, NORMAN |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University College of Swansea |
dc.contributor.author.fl_str_mv |
GREGÓRIO, ELISA A. [UNESP] A.RATCLIFFE, NORMAN |
dc.subject.por.fl_str_mv |
prophenoloxidase. haemolymph. insect immunity Rhodnius prolixus. Trypanosoma rangeli Triatoma infestans |
topic |
prophenoloxidase. haemolymph. insect immunity Rhodnius prolixus. Trypanosoma rangeli Triatoma infestans |
description |
Summary The presence of the prophenoloxidase (proPO) system in the haemolymph of Rhodnius protixus and Triatoma infestans and the role played by Trypanosoma rangeli in the in vitro activation of proPO were tested. Both R. prolixus and T. infestans whole blood preparations showed a very active ProPO system. The proPO cascade of the two insect species were differentially activated by microbial‐derived extracts: laminarin was a better activator of T. infestans haemolymph than of R. prolixus blood, and lipopolysaccharides from Shigella flexneri or Pseudomonas aeroginasa caused significant proPO activation of T. infestans haemolymph but not of R. prolixus preparations. For the two insect species, neither T. rangeli from culture nor parasite lysates were able to trigger proPO activation. The presence of the parasite in R. prolixus haemolymph/laminarin assays, however, significantly reduced the level of proPO activation to that of spontaneous activating controls. The immobilization of T. rangeli in vitro in haemolymph preparations occurred in both insect species and was dependent on the proPO activation intensity Our results suggest that the susceptibility of R. prolixus to T. rangeli haemocoel infection may be explained, at least in part, by the suppression of the insect immune defence system i.e., inhibition of proPO in the presence of this protozoan parasite. Copyright © 1991, Wiley Blackwell. All rights reserved |
publishDate |
1991 |
dc.date.none.fl_str_mv |
1991-01-01 2022-04-28T18:54:00Z 2022-04-28T18:54:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1365-3024.1991.tb00551.x Parasite Immunology, v. 13, n. 5, p. 551-564, 1991. 1365-3024 0141-9838 http://hdl.handle.net/11449/219147 10.1111/j.1365-3024.1991.tb00551.x 2-s2.0-0025913319 |
url |
http://dx.doi.org/10.1111/j.1365-3024.1991.tb00551.x http://hdl.handle.net/11449/219147 |
identifier_str_mv |
Parasite Immunology, v. 13, n. 5, p. 551-564, 1991. 1365-3024 0141-9838 10.1111/j.1365-3024.1991.tb00551.x 2-s2.0-0025913319 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasite Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
551-564 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129522668666880 |