HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland

Detalhes bibliográficos
Autor(a) principal: Damasceno, Karine Araújo
Data de Publicação: 2016
Outros Autores: Ferreira, Enio, Lima, Alessandra Estrela, Gamba, Conrado de Oliveira, Miranda, Fernanda Freitas, Alves, Mariana Rezende, Rocha, Rafael Malagoli, Barros, André Luis Branco de, Cassali, Geovanni Dantas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17751
Resumo: Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Conselho Nacional de Desenvolvimento e Pesquisa (CNPq) and Fundação de amparo a pesquisa do Estado de Minas Gerais (FAPEMIG).
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spelling Damasceno, Karine AraújoFerreira, EnioLima, Alessandra EstrelaGamba, Conrado de OliveiraMiranda, Fernanda FreitasAlves, Mariana RezendeRocha, Rafael MalagoliBarros, André Luis Branco deCassali, Geovanni Dantas2017-02-08T17:54:20Z2017-02-08T17:54:20Z2016DAMASCENO, K. A. et al. HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland. PLoS ONE, v. 11, n. 8, p. e0160419, 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1775110.1371/journal.pone.0160419Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Conselho Nacional de Desenvolvimento e Pesquisa (CNPq) and Fundação de amparo a pesquisa do Estado de Minas Gerais (FAPEMIG).Universidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, BrasilUniversidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, BrasilUniversidade Federal da Bahia. School of Veterinary Medicine and Zootechny. Department of Pathology and Clinics. Salvador, BA, BrazilUniversidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, BrasilInternational Center of Research in Cancer (CIPE). A.C. Camargo Cancer Center. São Paulo, SP, BrazilInternational Center of Research in Cancer (CIPE). A.C. Camargo Cancer Center. São Paulo, SP, BrazilUniversidade Federal de Minas Gerais. School of Pharmacy. Department of Clinical and Toxicological Analyses. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Department of General Pathology. Biological Sciences Institute. Belo Horizonte, MG, BrasilVersican expression promotes tumor growth by destabilizing focal cell contacts, thus impeding cell adhesion and facilitating cell migration. It not only presents or recruits molecules to the cell surface, but also modulates gene expression levels and coordinates complex signal pathways. Previously, we suggested that the interaction between versican and human epidermal growth factor receptors may be directly associated with tumor aggressiveness. Thus, the expression of EGFR and HER-2 in these neoplasms may contribute to a better understanding of the progression mechanisms in malignant mammary tumors. The purpose of this study was to correlate the gene and protein expressions of EGFR and HER2 by RNA In Situ Hybridization (ISH) and immunohistochemistry (IHC), respectively, and their relationship with the versican expression in carcinomas in mixed tumors and carcinosarcomas of the canine mammary gland. The results revealed that EGFR mRNA expression showed a significant difference between in situ and invasive carcinomatous areas in low and high versican expression groups. Identical results were observed in HER-2 mRNA expression. In immunohistochemistry analysis, neoplasms with low versican expression showed greater EGFR immunostaining in the in situ areas than in invasive areas, even as the group presenting high versican expression displayed greater EGFR and HER-2 staining in in situ areas. Significant EGFR and HER-2 mRNA and protein expressions in in situ carcinomatous sites relative to invasive areas suggest that these molecules play a role during the early stages of tumor progression.engPublic Library of ScienceGlandulas mamarias de animaisGenes EGFRCarcinomaProteínasTumores mamarios animaisAnimaisAnimal mammary glandsGene EGFRCarcinomaProteinsAnimal mammary tumorsAnimalHER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Glandinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17751/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDamasceno KA HER-2 and EGFR....pdfDamasceno KA HER-2 and 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dc.title.pt_BR.fl_str_mv HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland
title HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland
spellingShingle HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland
Damasceno, Karine Araújo
Glandulas mamarias de animais
Genes EGFR
Carcinoma
Proteínas
Tumores mamarios animais
Animais
Animal mammary glands
Gene EGFR
Carcinoma
Proteins
Animal mammary tumors
Animal
title_short HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland
title_full HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland
title_fullStr HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland
title_full_unstemmed HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland
title_sort HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland
author Damasceno, Karine Araújo
author_facet Damasceno, Karine Araújo
Ferreira, Enio
Lima, Alessandra Estrela
Gamba, Conrado de Oliveira
Miranda, Fernanda Freitas
Alves, Mariana Rezende
Rocha, Rafael Malagoli
Barros, André Luis Branco de
Cassali, Geovanni Dantas
author_role author
author2 Ferreira, Enio
Lima, Alessandra Estrela
Gamba, Conrado de Oliveira
Miranda, Fernanda Freitas
Alves, Mariana Rezende
Rocha, Rafael Malagoli
Barros, André Luis Branco de
Cassali, Geovanni Dantas
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Damasceno, Karine Araújo
Ferreira, Enio
Lima, Alessandra Estrela
Gamba, Conrado de Oliveira
Miranda, Fernanda Freitas
Alves, Mariana Rezende
Rocha, Rafael Malagoli
Barros, André Luis Branco de
Cassali, Geovanni Dantas
dc.subject.other.pt_BR.fl_str_mv Glandulas mamarias de animais
Genes EGFR
Carcinoma
Proteínas
Tumores mamarios animais
Animais
topic Glandulas mamarias de animais
Genes EGFR
Carcinoma
Proteínas
Tumores mamarios animais
Animais
Animal mammary glands
Gene EGFR
Carcinoma
Proteins
Animal mammary tumors
Animal
dc.subject.en.pt_BR.fl_str_mv Animal mammary glands
Gene EGFR
Carcinoma
Proteins
Animal mammary tumors
Animal
description Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Conselho Nacional de Desenvolvimento e Pesquisa (CNPq) and Fundação de amparo a pesquisa do Estado de Minas Gerais (FAPEMIG).
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-02-08T17:54:20Z
dc.date.available.fl_str_mv 2017-02-08T17:54:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DAMASCENO, K. A. et al. HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland. PLoS ONE, v. 11, n. 8, p. e0160419, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17751
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0160419
identifier_str_mv DAMASCENO, K. A. et al. HER-2 and EGFR mRNA Expression and Its Relationship with Versican in Malignant Matrix-Producing Tumors of the Canine Mammary Gland. PLoS ONE, v. 11, n. 8, p. e0160419, 2016.
1932-6203
10.1371/journal.pone.0160419
url https://www.arca.fiocruz.br/handle/icict/17751
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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