The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland

Detalhes bibliográficos
Autor(a) principal: Gamba, Conrado de Oliveira
Data de Publicação: 2019
Outros Autores: Damasceno, Karine Araújo, Ferreira, Izabel Cristina, Rodrigues, Michele Angela, Gomes, Dawidson Assis, Alves, Mariana Rezende, Rocha, Rafael Malagoli, Lima, Alessandra Estrela, Ferreira, Enio, Cassali, Geovanni Dantas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1371/journal.pone.0209497
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31772
Resumo: Centro de Aquisição e Processamento de Imagens” (CAPI- ICB/UFMG) for providing the microscopes to immunofluorescence evaluations.
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spelling Gamba, Conrado de OliveiraDamasceno, Karine AraújoFerreira, Izabel CristinaRodrigues, Michele AngelaGomes, Dawidson AssisAlves, Mariana RezendeRocha, Rafael MalagoliLima, Alessandra EstrelaFerreira, EnioCassali, Geovanni Dantas2019-02-19T17:58:49Z2019-02-19T17:58:49Z2019GAMBA, Conrado de Oliveira et al. The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland. Plos One, v. 14, n. 1, p. 1-15, 2019.1932-6203https://www.arca.fiocruz.br/handle/icict/3177210.1371/journal.pone.0209497Centro de Aquisição e Processamento de Imagens” (CAPI- ICB/UFMG) for providing the microscopes to immunofluorescence evaluations.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil / Universidade do Oeste de Santa Catarina. Curso de Medicina Veterinária. Campos Novos, SC, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologial. Belo Horizonte, MG, Brasil.International Center of Research in Cancer. A.C. Camargo Cancer Center. São Paulo, SP, Brazil.International Center of Research in Cancer. A.C. Camargo Cancer Center. São Paulo, SP, Brazil.Universidade Federal da Bahia. Escola de Medicina Veterinária. Departamento de Patologia e Clínicas. Salvador, BA, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil.The E-cadherin loss has frequently been associated with transcriptional repression mediated by transcription factors, such as the Zinc Finger E-Box Binding Homeobox-2 (ZEB2). Invasive micropapillary carcinomas (IMPCs) of the breast are aggressive neoplasms frequently related to lymph node metastasis and poor overall survival. In the canine mammary gland, IMPCs has just been reported and, based on its behavioral similarity with the human IMPCs, appears to be a good spontaneous model to this human entity. This study aimed to evaluate the relationship between E-cadherin and ZEB2 in a spontaneous canine model of invasive micropapillary carcinoma of the mammary gland. The correlation among gene expression (ZEB2 and CDH1) and clinicopathological findings was also explored. Nineteen cases of IMPC of the canine mammary gland were obtained, protein and mRNA expression were investigated through immunohistochemistry and RNA In Situ Hybridization, respectively. To better understand the relationship between E-cadherin and ZEB2, immunofluorescence was performed in canine IMPCs. Immunohistochemically, most of IMPCs showed 1+ (14/19, 73.7%) for E-cadherin; and positivity for ZEB2 was diagnosed in 47.4% of the IMPCs. Regarding the RNA In Situ Hybridization (ISH), most of IMPCs showed 4+ and 0+ for E-cadherin (CDH1) and ZEB2 respectively. Through immunofluorescence, the first and second more frequent combinatorial group were E-cadherin+ZEB2- and E-cadherin+ZEB2+; neoplastic cells showing concomitantly weak expression for E-cadherin and positivity for ZEB2 were frequently observed. A negative correlation was observed between E-cadherin and progesterone receptor expression in IMPCs. Based on these results, canine mammary IMPCs show E-cadherin lost and, at times reveals nuclear positivity for the transcription factor ZEB2 that seems to exert transcriptional repression of the CDH1.engPublic Library of ScienceCarcinomaGlandulas mamárias de animalNeoplasias da mamaE-caderinaAnimaisCarcinomaMammary Glands AnimalBreast NeoplasmsE-cadherinAnimalsThe investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary glandinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31772/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGamba C.O. The investigation of transcriptional....2019.pdfGamba C.O. The investigation of transcriptional....2019.pdfapplication/pdf1832458https://www.arca.fiocruz.br/bitstream/icict/31772/2/Gamba%20C.O.%20The%20investigation%20of%20transcriptional....2019.pdf30cd04e8056ef88e48b16a1429b9fe48MD52TEXTGamba C.O. The investigation of transcriptional....2019.pdf.txtGamba C.O. 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dc.title.pt_BR.fl_str_mv The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland
title The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland
spellingShingle The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland
Gamba, Conrado de Oliveira
Carcinoma
Glandulas mamárias de animal
Neoplasias da mama
E-caderina
Animais
Carcinoma
Mammary Glands Animal
Breast Neoplasms
E-cadherin
Animals
title_short The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland
title_full The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland
title_fullStr The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland
title_full_unstemmed The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland
title_sort The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland
author Gamba, Conrado de Oliveira
author_facet Gamba, Conrado de Oliveira
Damasceno, Karine Araújo
Ferreira, Izabel Cristina
Rodrigues, Michele Angela
Gomes, Dawidson Assis
Alves, Mariana Rezende
Rocha, Rafael Malagoli
Lima, Alessandra Estrela
Ferreira, Enio
Cassali, Geovanni Dantas
author_role author
author2 Damasceno, Karine Araújo
Ferreira, Izabel Cristina
Rodrigues, Michele Angela
Gomes, Dawidson Assis
Alves, Mariana Rezende
Rocha, Rafael Malagoli
Lima, Alessandra Estrela
Ferreira, Enio
Cassali, Geovanni Dantas
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gamba, Conrado de Oliveira
Damasceno, Karine Araújo
Ferreira, Izabel Cristina
Rodrigues, Michele Angela
Gomes, Dawidson Assis
Alves, Mariana Rezende
Rocha, Rafael Malagoli
Lima, Alessandra Estrela
Ferreira, Enio
Cassali, Geovanni Dantas
dc.subject.other.pt_BR.fl_str_mv Carcinoma
Glandulas mamárias de animal
Neoplasias da mama
E-caderina
Animais
topic Carcinoma
Glandulas mamárias de animal
Neoplasias da mama
E-caderina
Animais
Carcinoma
Mammary Glands Animal
Breast Neoplasms
E-cadherin
Animals
dc.subject.en.pt_BR.fl_str_mv Carcinoma
Mammary Glands Animal
Breast Neoplasms
E-cadherin
Animals
description Centro de Aquisição e Processamento de Imagens” (CAPI- ICB/UFMG) for providing the microscopes to immunofluorescence evaluations.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-02-19T17:58:49Z
dc.date.available.fl_str_mv 2019-02-19T17:58:49Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv GAMBA, Conrado de Oliveira et al. The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland. Plos One, v. 14, n. 1, p. 1-15, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31772
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0209497
identifier_str_mv GAMBA, Conrado de Oliveira et al. The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland. Plos One, v. 14, n. 1, p. 1-15, 2019.
1932-6203
10.1371/journal.pone.0209497
url https://www.arca.fiocruz.br/handle/icict/31772
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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