In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis

Detalhes bibliográficos
Autor(a) principal: Alem, Diego
Data de Publicação: 2014
Outros Autores: Dellavalle, Paola Diaz, Leoni, Carolina, De Simone, Salvatore Giovanni, Correa, Agustin, Oppezzo, Pablo, Rizza, Marco Dalla
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10169
Resumo: Instituto Nacional de Investigación Agropecuaria. Estación Experimental Wilson Ferreira Aldunate. Unidad de Biotecnología. Laboratorio de Proteínas. Canelones, Uruguay.
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spelling Alem, DiegoDellavalle, Paola DiazLeoni, CarolinaDe Simone, Salvatore GiovanniCorrea, AgustinOppezzo, PabloRizza, Marco Dalla2015-05-04T16:34:33Z2015-05-04T16:34:33Z2014ALEM, Diego et al. In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis. Microbial & Biochemical Technology, v. 6, n. 5, p. 268-273, 2014.1948-5948https://www.arca.fiocruz.br/handle/icict/1016910.4172/1948-5948.1000155engOmics InternationalIn search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Investigación Agropecuaria. Estación Experimental Wilson Ferreira Aldunate. Unidad de Biotecnología. Laboratorio de Proteínas. Canelones, Uruguay.Universidad Catolica del Uruguay. Facultad de Ingeniería y Tecnologías. Departamento de Ciencia y Tecnología de los Alimentos. Montevideo, Uruguay.Instituto Nacional de Investigación Agropecuaria. Estación Experimental Wilson Ferreira Aldunate. Programa Nacional de Investigación de Producción y Sustentabilidad Ambiental. Sección Protección Vegetal. Canelones, Uruguay.Fundação Oswaldo Cruz. Centro para Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil / Institut Pasteur de Montevideo. Unidad de Proteínas Recombinantes. Montevideo, Uruguay.Institut Pasteur de Montevideo. Unidad de Proteínas Recombinantes. Montevideo, Uruguay.Institut Pasteur de Montevideo. Unidad de Proteínas Recombinantes. Montevideo, Uruguay.Instituto Nacional de Investigación Agropecuaria. Estación Experimental Wilson Ferreira Aldunate. Unidad de Biotecnología. Laboratorio de Proteínas. Canelones, Uruguay.Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq-AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For topical application we present an AMP with gathering no hemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, pH-conditions and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easily to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially those in relation related to molecule bioavailability.Antimicrobial peptidesAlternaria solaniFusarium oxysporum f. sp. lycopersiciPenicillium sp.Heterologous expressionPeptídeosPenicilliumAlternariainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10169/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALsalvatore_simonietal_IOC_2014.pdfapplication/pdf981658https://www.arca.fiocruz.br/bitstream/icict/10169/2/salvatore_simonietal_IOC_2014.pdf8bbfbb23ad37c55a3b8b4a30f0147fa3MD52TEXTsalvatore_simonietal_IOC_2014.pdf.txtsalvatore_simonietal_IOC_2014.pdf.txtExtracted texttext/plain40869https://www.arca.fiocruz.br/bitstream/icict/10169/3/salvatore_simonietal_IOC_2014.pdf.txtb66cc3e8f6f8365e795f019fd66d0a85MD53icict/101692023-07-28 11:34:09.063oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-28T14:34:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis
title In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis
spellingShingle In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis
Alem, Diego
Antimicrobial peptides
Alternaria solani
Fusarium oxysporum f. sp. lycopersici
Penicillium sp.
Heterologous expression
Peptídeos
Penicillium
Alternaria
title_short In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis
title_full In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis
title_fullStr In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis
title_full_unstemmed In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis
title_sort In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis
author Alem, Diego
author_facet Alem, Diego
Dellavalle, Paola Diaz
Leoni, Carolina
De Simone, Salvatore Giovanni
Correa, Agustin
Oppezzo, Pablo
Rizza, Marco Dalla
author_role author
author2 Dellavalle, Paola Diaz
Leoni, Carolina
De Simone, Salvatore Giovanni
Correa, Agustin
Oppezzo, Pablo
Rizza, Marco Dalla
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alem, Diego
Dellavalle, Paola Diaz
Leoni, Carolina
De Simone, Salvatore Giovanni
Correa, Agustin
Oppezzo, Pablo
Rizza, Marco Dalla
dc.subject.en.pt_BR.fl_str_mv Antimicrobial peptides
Alternaria solani
Fusarium oxysporum f. sp. lycopersici
Penicillium sp.
Heterologous expression
topic Antimicrobial peptides
Alternaria solani
Fusarium oxysporum f. sp. lycopersici
Penicillium sp.
Heterologous expression
Peptídeos
Penicillium
Alternaria
dc.subject.decs.pt_BR.fl_str_mv Peptídeos
Penicillium
Alternaria
description Instituto Nacional de Investigación Agropecuaria. Estación Experimental Wilson Ferreira Aldunate. Unidad de Biotecnología. Laboratorio de Proteínas. Canelones, Uruguay.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-04T16:34:33Z
dc.date.available.fl_str_mv 2015-05-04T16:34:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALEM, Diego et al. In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis. Microbial & Biochemical Technology, v. 6, n. 5, p. 268-273, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10169
dc.identifier.issn.pt_BR.fl_str_mv 1948-5948
dc.identifier.doi.pt_BR.fl_str_mv 10.4172/1948-5948.1000155
identifier_str_mv ALEM, Diego et al. In search of topical agricultural biofungicides: properties of the recombinant antimicrobial peptide Trxaq-AMP obtained from amaranthus quitensis. Microbial & Biochemical Technology, v. 6, n. 5, p. 268-273, 2014.
1948-5948
10.4172/1948-5948.1000155
url https://www.arca.fiocruz.br/handle/icict/10169
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Omics International
publisher.none.fl_str_mv Omics International
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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