Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected

Bibliographic Details
Main Author: Cumberworth, Stephanie L.
Publication Date: 2017
Other Authors: Barrie, Jennifer A., Cunningham, Madeleine E., Figueiredo, Daniely Paulino Gomes de, Schultz, Verena, Wilder-Smith, Adrian J., Brennan, Benjamin, Pena, Lindomar J., França, Rafael Freitas de Oliveira, Linington, Christopher, Barnett, Susan C., Willison, Hugh J., Kohl, Alain, Edgar, Julia M.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/23933
Summary: Vídeo Relacionado ao artigo: https://www.youtube.com/watch?v=Tpc3rW9zsb8
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spelling Cumberworth, Stephanie L.Barrie, Jennifer A.Cunningham, Madeleine E.Figueiredo, Daniely Paulino Gomes deSchultz, VerenaWilder-Smith, Adrian J.Brennan, BenjaminPena, Lindomar J.França, Rafael Freitas de OliveiraLinington, ChristopherBarnett, Susan C.Willison, Hugh J.Kohl, AlainEdgar, Julia M.2018-01-17T12:44:37Z2018-01-17T12:44:37Z2017CUMBERWORTH, S. L. et al. Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected. Acta Neuropathologica Communications, v. 5, n. 1, p. 1-16, Dec. 2017.2051-5960https://www.arca.fiocruz.br/handle/icict/2393310.1186/s40478-017-0450-8Vídeo Relacionado ao artigo: https://www.youtube.com/watch?v=Tpc3rW9zsb8Este estudo foi financiado pelo UK Medical Research Council [MC_UU_12014, MR / N017552 / 1 (AK); Resposta rápida ZIKA MC_PC_15105 (HW, JME, AK); MR / K501335 / 1 (Bolsa de Treinamento de Doutorado, SLC)], Centro Nacional de Reposição, Redução e Refinamento (NC / L000423 / 1, JME, CL) e Wellcome Trust (203680 / Z / 16 / Z, WT092805; HJW). Este projecto foi parcialmente financiado através do programa de investigação e inovação Horizonte 2020 da União Europeia no âmbito do acordo de subvenção ZikaPLAN n.º 734584 (HJW, JME, SB, CL).MRC-University of Glasgow. Centre for Virus Research. G61 1QH. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.MRC-University of Glasgow. Centre for Virus Research. G61 1QH. Glasgow, Scotland, UK.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.MRC-University of Glasgow. Centre for Virus Research. G61 1QH. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK / Max Planck Institute for Experimental Medicine. Department of Neurogenetics. Goettingen, Germany.The recent global outbreak of Zika virus (ZIKV) infection has been linked to severe neurological disorders affecting the peripheral and central nervous systems (PNS and CNS, respectively). The pathobiology underlying these diverse clinical phenotypes are the subject of intense research; however, even the principal neural cell types vulnerable to productive Zika infection remain poorly characterised. Here we used CNS and PNS myelinating cultures from wild type and Ifnar1 knockout mice to examine neuronal and glial tropism and short-term consequences of direct infection with a Brazilian variant of ZIKV. Cell cultures were infected pre- or post-myelination for various intervals, then stained with cell-type and ZIKV-specific antibodies. In bypassing systemic immunity using ex vivo culture, and the type I interferon response in Ifnar1 deficient cells, we were able to evaluate the intrinsic infectivity of neural cells. Through systematic quantification of ZIKV infected cells in myelinating cultures, we found that ZIKV infection is enhanced in the absence of the type I interferon responses and that CNS cells are considerably more susceptible to infection than PNS cells. In particular, we demonstrate that CNS axons and myelinating oligodendrocytes are especially vulnerable to injury. These results have implications for understanding the pathobiology of neurological symptoms associated with ZIKV infection. Furthermore, we provide a quantifiable ex vivo infection model that can be used for fundamental and therapeutic studies on viral neuroinvasion and its consequences.engAxonSistema nervoso centralMurineMielinaSistema nervoso periféricoTropismoVírus ZikaAxonCentral nervous systemMurineMyelinPeripheral nervous systemTropismZika virusZika virusSistema NervosoZika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affectedinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23933/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALIAM - Zika virus tropism and interactions in myelinating neural cell cultures ....pdfIAM - Zika virus tropism and interactions in myelinating neural cell cultures ....pdfapplication/pdf10397042https://www.arca.fiocruz.br/bitstream/icict/23933/2/IAM%20-%20Zika%20virus%20tropism%20and%20interactions%20in%20myelinating%20neural%20cell%20cultures%20....pdf2624113b850489dd1f803ee5fd981bcaMD52TEXTIAM - 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dc.title.pt_BR.fl_str_mv Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected
title Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected
spellingShingle Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected
Cumberworth, Stephanie L.
Axon
Sistema nervoso central
Murine
Mielina
Sistema nervoso periférico
Tropismo
Vírus Zika
Axon
Central nervous system
Murine
Myelin
Peripheral nervous system
Tropism
Zika virus
Zika virus
Sistema Nervoso
title_short Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected
title_full Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected
title_fullStr Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected
title_full_unstemmed Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected
title_sort Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected
author Cumberworth, Stephanie L.
author_facet Cumberworth, Stephanie L.
Barrie, Jennifer A.
Cunningham, Madeleine E.
Figueiredo, Daniely Paulino Gomes de
Schultz, Verena
Wilder-Smith, Adrian J.
Brennan, Benjamin
Pena, Lindomar J.
França, Rafael Freitas de Oliveira
Linington, Christopher
Barnett, Susan C.
Willison, Hugh J.
Kohl, Alain
Edgar, Julia M.
author_role author
author2 Barrie, Jennifer A.
Cunningham, Madeleine E.
Figueiredo, Daniely Paulino Gomes de
Schultz, Verena
Wilder-Smith, Adrian J.
Brennan, Benjamin
Pena, Lindomar J.
França, Rafael Freitas de Oliveira
Linington, Christopher
Barnett, Susan C.
Willison, Hugh J.
Kohl, Alain
Edgar, Julia M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cumberworth, Stephanie L.
Barrie, Jennifer A.
Cunningham, Madeleine E.
Figueiredo, Daniely Paulino Gomes de
Schultz, Verena
Wilder-Smith, Adrian J.
Brennan, Benjamin
Pena, Lindomar J.
França, Rafael Freitas de Oliveira
Linington, Christopher
Barnett, Susan C.
Willison, Hugh J.
Kohl, Alain
Edgar, Julia M.
dc.subject.other.pt_BR.fl_str_mv Axon
Sistema nervoso central
Murine
Mielina
Sistema nervoso periférico
Tropismo
Vírus Zika
topic Axon
Sistema nervoso central
Murine
Mielina
Sistema nervoso periférico
Tropismo
Vírus Zika
Axon
Central nervous system
Murine
Myelin
Peripheral nervous system
Tropism
Zika virus
Zika virus
Sistema Nervoso
dc.subject.en.pt_BR.fl_str_mv Axon
Central nervous system
Murine
Myelin
Peripheral nervous system
Tropism
Zika virus
dc.subject.decs.pt_BR.fl_str_mv Zika virus
Sistema Nervoso
description Vídeo Relacionado ao artigo: https://www.youtube.com/watch?v=Tpc3rW9zsb8
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-01-17T12:44:37Z
dc.date.available.fl_str_mv 2018-01-17T12:44:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CUMBERWORTH, S. L. et al. Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected. Acta Neuropathologica Communications, v. 5, n. 1, p. 1-16, Dec. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23933
dc.identifier.issn.pt_BR.fl_str_mv 2051-5960
dc.identifier.doi.none.fl_str_mv 10.1186/s40478-017-0450-8
identifier_str_mv CUMBERWORTH, S. L. et al. Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected. Acta Neuropathologica Communications, v. 5, n. 1, p. 1-16, Dec. 2017.
2051-5960
10.1186/s40478-017-0450-8
url https://www.arca.fiocruz.br/handle/icict/23933
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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