Amiodarone inhibits Trypanosoma cruzi infection and promotes cardiac cell recovery with gap junction and cytoskeleton reassembly in vitro

Detalhes bibliográficos
Autor(a) principal: Adesse, Daniel
Data de Publicação: 2011
Outros Autores: Azzam, Eduardo Meirelles, Meirelles, Maria de Nazareth L., Urbina, Julio A., Garzoni, Luciana R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27993
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.
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spelling Adesse, DanielAzzam, Eduardo MeirellesMeirelles, Maria de Nazareth L.Urbina, Julio A.Garzoni, Luciana R.2018-08-08T11:36:02Z2018-08-08T11:36:02Z2011ADESSE, Daniel; et al. Amiodarone Inhibits Trypanosoma cruzi Infection and Promotes Cardiac Cell Recovery with Gap Junction and Cytoskeleton Reassembly In Vitro. Antimicrobial Agents and Chemotherapy, v.55, n.1, p.203-210, Jan. 2011.0066-4804https://www.arca.fiocruz.br/handle/icict/2799310.1128/AAC.01129-101098-6596engAmerican Society for MicrobiologyTrypanosoma CruziDoença de ChagasDoenças cardíacasAmiodaronaTrypanosoma CruziChagas DiseaseamiodaroneHeart diseasesAmiodarone inhibits Trypanosoma cruzi infection and promotes cardiac cell recovery with gap junction and cytoskeleton reassembly in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.Instituto Venezolano de Investigaciones Cientificas. Centro de Biofísica y Bioquímica. Laboratório de Química Biológica. Caracas, Venezuela.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.We present the results of the first detailed study of the antiproliferative and ultrastructural effects of amiodarone on Trypanosoma cruzi, the causative agent of Chagas' disease. Moreover, we report the effects of this compound on the recovery of F-actin fibrils, connexin43, and contractility in T. cruzi-infected cardiac myocytes. Amiodarone is the most prescribed class III antiarrhythmic agent and is frequently used for the symptomatic treatment of Chagas' disease patients with cardiac compromise. In addition, recent studies identified its antifungal and antiprotozoal activities, which take place through Ca(2+) homeostasis disruption and ergosterol biosynthesis blockade. We tested different concentrations of amiodarone (2.5 to 10 μM) on infected primary cultures of heart muscle cells and observed a dose- and time-dependent effect on growth of the clinically relevant intracellular amastigote form of T. cruzi. Ultrastructural analyses revealed that amiodarone had a profound effect on intracellular amastigotes, including mitochondrial swelling and disorganization of reservosomes and the kinetoplast and a blockade of amastigote-trypomastigote differentiation. Amiodarone showed no toxic effects on host cells, which recovered their F-actin fibrillar organization, connexin43 distribution, and spontaneous contractility concomitant with the drug-induced eradication of the intracellular parasites. Amiodarone is, therefore, a promising compound for the development of new drugs against T. cruzi.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27993/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALdaniel _adesse_etal_IOC_2010.pdfdaniel _adesse_etal_IOC_2010.pdfapplication/pdf6190363https://www.arca.fiocruz.br/bitstream/icict/27993/2/daniel%20_adesse_etal_IOC_2010.pdf186c26faeff51157c4b30590631545dbMD52TEXTdaniel _adesse_etal_IOC_2010.pdf.txtdaniel _adesse_etal_IOC_2010.pdf.txtExtracted texttext/plain34229https://www.arca.fiocruz.br/bitstream/icict/27993/3/daniel%20_adesse_etal_IOC_2010.pdf.txte2f74dedc037db81b376ef4409d8fcafMD53icict/279932018-08-15 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dc.title.pt_BR.fl_str_mv Amiodarone inhibits Trypanosoma cruzi infection and promotes cardiac cell recovery with gap junction and cytoskeleton reassembly in vitro
title Amiodarone inhibits Trypanosoma cruzi infection and promotes cardiac cell recovery with gap junction and cytoskeleton reassembly in vitro
spellingShingle Amiodarone inhibits Trypanosoma cruzi infection and promotes cardiac cell recovery with gap junction and cytoskeleton reassembly in vitro
Adesse, Daniel
Trypanosoma Cruzi
Doença de Chagas
Doenças cardíacas
Amiodarona
Trypanosoma Cruzi
Chagas Disease
amiodarone
Heart diseases
title_short Amiodarone inhibits Trypanosoma cruzi infection and promotes cardiac cell recovery with gap junction and cytoskeleton reassembly in vitro
title_full Amiodarone inhibits Trypanosoma cruzi infection and promotes cardiac cell recovery with gap junction and cytoskeleton reassembly in vitro
title_fullStr Amiodarone inhibits Trypanosoma cruzi infection and promotes cardiac cell recovery with gap junction and cytoskeleton reassembly in vitro
title_full_unstemmed Amiodarone inhibits Trypanosoma cruzi infection and promotes cardiac cell recovery with gap junction and cytoskeleton reassembly in vitro
title_sort Amiodarone inhibits Trypanosoma cruzi infection and promotes cardiac cell recovery with gap junction and cytoskeleton reassembly in vitro
author Adesse, Daniel
author_facet Adesse, Daniel
Azzam, Eduardo Meirelles
Meirelles, Maria de Nazareth L.
Urbina, Julio A.
Garzoni, Luciana R.
author_role author
author2 Azzam, Eduardo Meirelles
Meirelles, Maria de Nazareth L.
Urbina, Julio A.
Garzoni, Luciana R.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Adesse, Daniel
Azzam, Eduardo Meirelles
Meirelles, Maria de Nazareth L.
Urbina, Julio A.
Garzoni, Luciana R.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma Cruzi
Doença de Chagas
Doenças cardíacas
Amiodarona
topic Trypanosoma Cruzi
Doença de Chagas
Doenças cardíacas
Amiodarona
Trypanosoma Cruzi
Chagas Disease
amiodarone
Heart diseases
dc.subject.en.pt_BR.fl_str_mv Trypanosoma Cruzi
Chagas Disease
amiodarone
Heart diseases
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2018-08-08T11:36:02Z
dc.date.available.fl_str_mv 2018-08-08T11:36:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv ADESSE, Daniel; et al. Amiodarone Inhibits Trypanosoma cruzi Infection and Promotes Cardiac Cell Recovery with Gap Junction and Cytoskeleton Reassembly In Vitro. Antimicrobial Agents and Chemotherapy, v.55, n.1, p.203-210, Jan. 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27993
dc.identifier.issn.pt_BR.fl_str_mv 0066-4804
dc.identifier.doi.none.fl_str_mv 10.1128/AAC.01129-10
dc.identifier.eissn.none.fl_str_mv 1098-6596
identifier_str_mv ADESSE, Daniel; et al. Amiodarone Inhibits Trypanosoma cruzi Infection and Promotes Cardiac Cell Recovery with Gap Junction and Cytoskeleton Reassembly In Vitro. Antimicrobial Agents and Chemotherapy, v.55, n.1, p.203-210, Jan. 2011.
0066-4804
10.1128/AAC.01129-10
1098-6596
url https://www.arca.fiocruz.br/handle/icict/27993
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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