The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins

Detalhes bibliográficos
Autor(a) principal: Valente, Richard Hemmi
Data de Publicação: 2018
Outros Autores: Sakai, Fernanda, Portes Junior, José Antonio, Viana, Luciana Godoy, Carneiro, Sylvia Mendes, Perales, Jonas, Yamanouye, Norma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/29110
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil.
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spelling Valente, Richard HemmiSakai, FernandaPortes Junior, José AntonioViana, Luciana GodoyCarneiro, Sylvia MendesPerales, JonasYamanouye, Norma2018-09-27T15:47:59Z2018-09-27T15:47:59Z2018VALENTE, Richard Hemmi; et al. The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins. Toxins, v.10, n.121, 13p, 2018.2072-6651https://www.arca.fiocruz.br/handle/icict/2911010.3390/toxins100301212072-6651engMDPIBothrops jararacaaparelho glandular de venenoduto primáriomorfologiaciclo secretorprodução de venenoproteômicavenom glandular apparatusprimary ductmorphologysecretory cyclevenom productionproteomicsBothrops jararacaThe Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil.Instituto Butantan. Laboratório de Farmacologia. São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Imunopatologia. São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Farmacologia. São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Biologia Celular. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil.Instituto Butantan. Laboratório de Farmacologia. São Paulo, SP, Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil.Despite numerous studies concerning morphology and venom production and secretion in the main venom gland (and some data on the accessory gland) of the venom glandular apparatus of Viperidae snakes, the primary duct has been overlooked. We characterized the primary duct of the Bothrops jararaca snake by morphological analysis, immunohistochemistry and proteomics. The duct has a pseudostratified epithelium with secretory columnar cells with vesicles of various electrondensities, as well as mitochondria-rich, dark, basal, and horizontal cells. Morphological analysis, at different periods after venom extraction, showed that the primary duct has a long cycle of synthesis and secretion, as do the main venom and accessory glands; however, the duct has a mixed mode venom storage, both in the lumen and in secretory vesicles. Mouse anti-B. jararaca venom serum strongly stained the primary duct's epithelium. Subsequent proteomic analysis revealed the synthesis of venom toxins-mainly C-type lectin/C-type lectin-like proteins. We propose that the primary duct's toxin synthesis products complement the final venom bolus. Finally, we hypothesize that the primary duct and the accessory gland (components of the venom glandular apparatus) are part of the evolutionary path from a salivary gland towards the main venom gland.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29110/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjoseantonio_portesjr_etal_IOC_2018.pdfjoseantonio_portesjr_etal_IOC_2018.pdfapplication/pdf6165008https://www.arca.fiocruz.br/bitstream/icict/29110/2/joseantonio_portesjr_etal_IOC_2018.pdfb14c5e58fb2db4ccaae9c736e6652f8fMD52TEXTjoseantonio_portesjr_etal_IOC_2018.pdf.txtjoseantonio_portesjr_etal_IOC_2018.pdf.txtExtracted texttext/plain59357https://www.arca.fiocruz.br/bitstream/icict/29110/3/joseantonio_portesjr_etal_IOC_2018.pdf.txt1f05869b794263eb5ca6f70257651207MD53icict/291102018-09-28 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dc.title.pt_BR.fl_str_mv The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins
title The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins
spellingShingle The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins
Valente, Richard Hemmi
Bothrops jararaca
aparelho glandular de veneno
duto primário
morfologia
ciclo secretor
produção de veneno
proteômica
venom glandular apparatus
primary duct
morphology
secretory cycle
venom production
proteomics
Bothrops jararaca
title_short The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins
title_full The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins
title_fullStr The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins
title_full_unstemmed The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins
title_sort The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins
author Valente, Richard Hemmi
author_facet Valente, Richard Hemmi
Sakai, Fernanda
Portes Junior, José Antonio
Viana, Luciana Godoy
Carneiro, Sylvia Mendes
Perales, Jonas
Yamanouye, Norma
author_role author
author2 Sakai, Fernanda
Portes Junior, José Antonio
Viana, Luciana Godoy
Carneiro, Sylvia Mendes
Perales, Jonas
Yamanouye, Norma
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Valente, Richard Hemmi
Sakai, Fernanda
Portes Junior, José Antonio
Viana, Luciana Godoy
Carneiro, Sylvia Mendes
Perales, Jonas
Yamanouye, Norma
dc.subject.other.pt_BR.fl_str_mv Bothrops jararaca
aparelho glandular de veneno
duto primário
morfologia
ciclo secretor
produção de veneno
proteômica
topic Bothrops jararaca
aparelho glandular de veneno
duto primário
morfologia
ciclo secretor
produção de veneno
proteômica
venom glandular apparatus
primary duct
morphology
secretory cycle
venom production
proteomics
Bothrops jararaca
dc.subject.en.pt_BR.fl_str_mv venom glandular apparatus
primary duct
morphology
secretory cycle
venom production
proteomics
Bothrops jararaca
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Toxinas. Brasília, DF, Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-09-27T15:47:59Z
dc.date.available.fl_str_mv 2018-09-27T15:47:59Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VALENTE, Richard Hemmi; et al. The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins. Toxins, v.10, n.121, 13p, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/29110
dc.identifier.issn.pt_BR.fl_str_mv 2072-6651
dc.identifier.doi.none.fl_str_mv 10.3390/toxins10030121
dc.identifier.eissn.none.fl_str_mv 2072-6651
identifier_str_mv VALENTE, Richard Hemmi; et al. The Primary Duct of Bothrops jararaca Glandular Apparatus Secretes Toxins. Toxins, v.10, n.121, 13p, 2018.
2072-6651
10.3390/toxins10030121
url https://www.arca.fiocruz.br/handle/icict/29110
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/29110/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/29110/2/joseantonio_portesjr_etal_IOC_2018.pdf
https://www.arca.fiocruz.br/bitstream/icict/29110/3/joseantonio_portesjr_etal_IOC_2018.pdf.txt
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