Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil

Detalhes bibliográficos
Autor(a) principal: Uehara, Aline Almeida
Data de Publicação: 2011
Outros Autores: Amorim, Efigenia de Lourdes Teixeira, Ferreira, Maria de Fatima, Andrade, Cláudia Ferreira de, Clementino, Maysa Beatriz Mandetta, Filippis, Ivano de, Neves, Felipe P. G., Pinto, Tatiana de C. A., Teixeira, Lucia Martins, Demarval, Marcia Giambiagi, Fracalanzza, Sérgio Eduardo Longo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1128/jcm.01175-11
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11029
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.
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spelling Uehara, Aline AlmeidaAmorim, Efigenia de Lourdes TeixeiraFerreira, Maria de FatimaAndrade, Cláudia Ferreira deClementino, Maysa Beatriz MandettaFilippis, Ivano deNeves, Felipe P. G.Pinto, Tatiana de C. A.Teixeira, Lucia MartinsDemarval, Marcia GiambiagiFracalanzza, Sérgio Eduardo Longo2015-06-30T18:34:02Z2015-06-30T18:34:02Z2011Uehara, Aline Almeida et al. Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil. Journal of Clinical Microbiology, v. 49, n. 12, p. 4208-4212.0095-1137https://www.arca.fiocruz.br/handle/icict/1102910.1128/jcm.01175-11engAmerican Society for MicrobiologyNeisseria gonorrhoeaeQuinolonasTestes de Sensibilidade MicrobianaMutação de Sentido IncorretoAnálise por ConglomeradosTipagem MolecularNeisseria gonorrhoeaeQuinolonesMicrobial Sensitivity TestsMutation, MissenseCluster AnalysisMolecular TypingNeisseria gonorrhoeaeQuinolonasTestes de Sensibilidade MicrobianaMutação de Sentido IncorretoAnálise por ConglomeradosTipagem MolecularMolecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Laboratório Sérgio Franco, Rio de Janeiro, RJ, Brasil.Laboratório Sérgio Franco, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Despite the rapid spread of antibiotic resistance among gonococci worldwide, limited reports are available from Brazilian locations. In the present study, 25 quinolone-resistant Neisseria gonorrhoeae (QRNG) strains isolated in Rio de Janeiro, Brazil, were characterized by phenotypic and molecular methods, including analysis of mutations in the gyrA and parC genes. They represented 16.5% of the N. gonorrhoeae isolates obtained during a survey performed from 2006 to 2010. A trend for increasing resistance to ciprofloxacin was observed in the period investigated. The most prevalent pattern of mutation observed among QRNG isolates, Ser-91 to Phe and Asp-95 to Gly in gyrA and Ser-87 to Arg in parC, was detected in 40% of the isolates exhibiting MICs ranging from 4 to >32 mug/ml. Rare types of mutations were found in the gyrA gene (Gln-102 to His [12%] and Asp-95 to Tyr [4%]) and in the parC gene (Ser-88 to Thr [4%]). The genetic relationship of the QRNG isolates, evaluated by pulsed-field gel electrophoresis, suggested that the increase in the frequencies of the QRNG isolates in Rio de Janeiro, Brazil, may have arisen as a result of simultaneous spread of two clonal groups. The results also indicate that fluoroquinolones may no longer be used as first line antibiotics for the treatment of gonorrhea in Rio de Janeiro, and that programs for antimicrobial susceptibility surveillance of N. gonorrhoeae should also be implemented in other regions of Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALJ._Clin_Microbiol_49_4208-12.pdfJ._Clin_Microbiol_49_4208-12.pdfapplication/pdf288782https://www.arca.fiocruz.br/bitstream/icict/11029/2/J._Clin_Microbiol_49_4208-12.pdf0d176fd506305eeacc9f1d9e6a2e6b37MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/11029/3/license.txt8b4c200b4e10021c5683c6ccaba07169MD53TEXTJ._Clin_Microbiol_49_4208-12.pdf.txtJ._Clin_Microbiol_49_4208-12.pdf.txtExtracted texttext/plain29746https://www.arca.fiocruz.br/bitstream/icict/11029/4/J._Clin_Microbiol_49_4208-12.pdf.txt8f68a809821bf4bfc3c354a9a4b28ca9MD54icict/110292018-04-04 08:06:21.286oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-04T11:06:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en.fl_str_mv Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil
title Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil
spellingShingle Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil
Uehara, Aline Almeida
Neisseria gonorrhoeae
Quinolonas
Testes de Sensibilidade Microbiana
Mutação de Sentido Incorreto
Análise por Conglomerados
Tipagem Molecular
Neisseria gonorrhoeae
Quinolones
Microbial Sensitivity Tests
Mutation, Missense
Cluster Analysis
Molecular Typing
Neisseria gonorrhoeae
Quinolonas
Testes de Sensibilidade Microbiana
Mutação de Sentido Incorreto
Análise por Conglomerados
Tipagem Molecular
title_short Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil
title_full Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil
title_fullStr Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil
title_full_unstemmed Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil
title_sort Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil
author Uehara, Aline Almeida
author_facet Uehara, Aline Almeida
Amorim, Efigenia de Lourdes Teixeira
Ferreira, Maria de Fatima
Andrade, Cláudia Ferreira de
Clementino, Maysa Beatriz Mandetta
Filippis, Ivano de
Neves, Felipe P. G.
Pinto, Tatiana de C. A.
Teixeira, Lucia Martins
Demarval, Marcia Giambiagi
Fracalanzza, Sérgio Eduardo Longo
author_role author
author2 Amorim, Efigenia de Lourdes Teixeira
Ferreira, Maria de Fatima
Andrade, Cláudia Ferreira de
Clementino, Maysa Beatriz Mandetta
Filippis, Ivano de
Neves, Felipe P. G.
Pinto, Tatiana de C. A.
Teixeira, Lucia Martins
Demarval, Marcia Giambiagi
Fracalanzza, Sérgio Eduardo Longo
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Uehara, Aline Almeida
Amorim, Efigenia de Lourdes Teixeira
Ferreira, Maria de Fatima
Andrade, Cláudia Ferreira de
Clementino, Maysa Beatriz Mandetta
Filippis, Ivano de
Neves, Felipe P. G.
Pinto, Tatiana de C. A.
Teixeira, Lucia Martins
Demarval, Marcia Giambiagi
Fracalanzza, Sérgio Eduardo Longo
dc.subject.other.pt_BR.fl_str_mv Neisseria gonorrhoeae
Quinolonas
Testes de Sensibilidade Microbiana
Mutação de Sentido Incorreto
Análise por Conglomerados
Tipagem Molecular
topic Neisseria gonorrhoeae
Quinolonas
Testes de Sensibilidade Microbiana
Mutação de Sentido Incorreto
Análise por Conglomerados
Tipagem Molecular
Neisseria gonorrhoeae
Quinolones
Microbial Sensitivity Tests
Mutation, Missense
Cluster Analysis
Molecular Typing
Neisseria gonorrhoeae
Quinolonas
Testes de Sensibilidade Microbiana
Mutação de Sentido Incorreto
Análise por Conglomerados
Tipagem Molecular
dc.subject.en.en.fl_str_mv Neisseria gonorrhoeae
Quinolones
Microbial Sensitivity Tests
Mutation, Missense
Cluster Analysis
Molecular Typing
dc.subject.decs.pt_BR.fl_str_mv Neisseria gonorrhoeae
Quinolonas
Testes de Sensibilidade Microbiana
Mutação de Sentido Incorreto
Análise por Conglomerados
Tipagem Molecular
description Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2015-06-30T18:34:02Z
dc.date.available.fl_str_mv 2015-06-30T18:34:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Uehara, Aline Almeida et al. Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil. Journal of Clinical Microbiology, v. 49, n. 12, p. 4208-4212.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11029
dc.identifier.issn.none.fl_str_mv 0095-1137
dc.identifier.doi.none.fl_str_mv 10.1128/jcm.01175-11
identifier_str_mv Uehara, Aline Almeida et al. Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil. Journal of Clinical Microbiology, v. 49, n. 12, p. 4208-4212.
0095-1137
10.1128/jcm.01175-11
url https://www.arca.fiocruz.br/handle/icict/11029
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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