Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles

Detalhes bibliográficos
Autor(a) principal: Guedes, Carlos Eduardo Sampaio
Data de Publicação: 2015
Outros Autores: Lima, José Geraldo Bomfim, Helfer, Emmanuèle, Veras, Patrícia Sampaio Tavares, Viallat, Annie
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13430
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
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spelling Guedes, Carlos Eduardo SampaioLima, José Geraldo BomfimHelfer, EmmanuèleVeras, Patrícia Sampaio TavaresViallat, Annie2016-03-31T18:38:54Z2016-03-31T18:38:54Z2015GUEDES, C. E. S. et al. Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles. Plos One, v. 10, n. 8, p. e0134925, 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1343010.1371/journal.pone.0134925engPublic Library of ScienceLeishmaniaMacrófagosLeishmania amazonensisMamíferosFagocitoseEncapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuolesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilAix-Marseille Université. CNRS. Marseille, FranceFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilAix-Marseille Université. CNRS. Marseille, FranceAfter phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate. Here, using a variant of the ‘inverted emulsion’ method, we succeeded in encapsulating living L. amazonensis parasites in giant artificial liposomes that serve as model PVs. We were able to control the size of liposomes, the pH and the composition of their internal volume, and the number of internalized parasites per liposome. L. amazonensis promastigotes encapsulated in liposomes filled with RPMI-Dextran solution at pH 7.5 or 6.5 survived up to 96 h at 24°C. At 37°C and pH 5.5, parasites survived 48h. This method paves the way to identifying certain effectors secreted by the parasite and to unraveling specific mechanisms of fusion between the PV and intracellular vesicles of the host cell. This method will also facilitate the study of the temporal evolution of biophysical properties of the PV during its maturation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13430/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGuedes, Carlos ES. Encapsulation of living....pdfGuedes, Carlos ES. Encapsulation of living....pdfapplication/pdf2009372https://www.arca.fiocruz.br/bitstream/icict/13430/2/Guedes%2c%20Carlos%20ES.%20Encapsulation%20of%20living....pdf6f0c9ddab1133616332ccf78a3cad494MD52TEXTGuedes, Carlos ES. Encapsulation of living....pdf.txtGuedes, Carlos ES. 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dc.title.pt_BR.fl_str_mv Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles
title Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles
spellingShingle Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles
Guedes, Carlos Eduardo Sampaio
Leishmania
Macrófagos
Leishmania amazonensis
Mamíferos
Fagocitose
title_short Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles
title_full Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles
title_fullStr Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles
title_full_unstemmed Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles
title_sort Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles
author Guedes, Carlos Eduardo Sampaio
author_facet Guedes, Carlos Eduardo Sampaio
Lima, José Geraldo Bomfim
Helfer, Emmanuèle
Veras, Patrícia Sampaio Tavares
Viallat, Annie
author_role author
author2 Lima, José Geraldo Bomfim
Helfer, Emmanuèle
Veras, Patrícia Sampaio Tavares
Viallat, Annie
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Guedes, Carlos Eduardo Sampaio
Lima, José Geraldo Bomfim
Helfer, Emmanuèle
Veras, Patrícia Sampaio Tavares
Viallat, Annie
dc.subject.other.pt_BR.fl_str_mv Leishmania
Macrófagos
Leishmania amazonensis
Mamíferos
Fagocitose
topic Leishmania
Macrófagos
Leishmania amazonensis
Mamíferos
Fagocitose
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-31T18:38:54Z
dc.date.available.fl_str_mv 2016-03-31T18:38:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUEDES, C. E. S. et al. Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles. Plos One, v. 10, n. 8, p. e0134925, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13430
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0134925
identifier_str_mv GUEDES, C. E. S. et al. Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles. Plos One, v. 10, n. 8, p. e0134925, 2015.
1932-6203
10.1371/journal.pone.0134925
url https://www.arca.fiocruz.br/handle/icict/13430
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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