Insecticide resistance in Aedes aegypti populations from Ceará, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/31254 |
Resumo: | National Council for Scientific and Technological Development (CNPq, Grants ID: 479825/2009-2 and 550116/2010-9), CAPES/PROCAD/NF 23, Rede Nordeste de Biotecnologia (RENORBIO) and Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE, Grant ID: APQ 1361-4.00/08). |
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Lima, Estelita PereiraPaiva, Marcelo Henrique SantosAraújo, Ana Paula deSilva, Ellyda Vanessa Gomes daSilva, Ulisses Mariano daOliveira, Lúcia Nogueira deSantana, Antonio Euzébio G.Barbosa, Clarisse NogueiraPaiva Neto, Clovis C. deGoulart, Marilia O. F.Wilding, Craig StephenAyres, Constância Flávia JunqueiraSantos, Maria Alice V. de Melo2019-01-23T14:29:11Z2019-01-23T14:29:11Z2011LIMA, Estelita Pereira et al. Insecticide Resistance in Aedes Aegypti Populations from Ceará, Brazil. Parasites & Vectors, v. 4, p. 5, 12 jan. 2011.1756-3305https://www.arca.fiocruz.br/handle/icict/3125410.1186/1756-3305-4-5National Council for Scientific and Technological Development (CNPq, Grants ID: 479825/2009-2 and 550116/2010-9), CAPES/PROCAD/NF 23, Rede Nordeste de Biotecnologia (RENORBIO) and Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE, Grant ID: APQ 1361-4.00/08).Universidade Federal do Ceará. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Faculdade ASCES. Caruaru, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Núcleo de Entomologia Médica do Cariri. Juazeiro do Norte, CE, Brasil.Núcleo de Entomologia Médica do Cariri. Juazeiro do Norte, CE, Brasil.Universidade Federal de Alagoas. Maceió, AL, Brasil.Universidade Federal do Ceará. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Fortaleza, CE, Brasil.Universidade Federal de Alagoas. Maceió, AL, Brasil.Liverpool School of Tropical Medicine. Vector Group. Pembroke Place, Liverpool, UK.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Background Organophosphates and pyrethroids are used widely in Brazil to control Aedes aegypti, the main vector of dengue viruses, under the auspices of the National Programme for Dengue Control. Resistance to these insecticides is widespread throughout Brazil. In Ceará the vector is present in 98% of districts and resistance to temephos has been reported previously. Here we measure resistance to temephos and the pyrethroid cypermethrin in three populations from Ceará and use biochemical and molecular assays to characterise resistance mechanisms. Results Resistance to temephos varied widely across the three studied populations, with resistance ratios (RR95) of 7.2, 30 and 192.7 in Juazeiro do Norte, Barbalha and Crato respectively. The high levels of resistance detected in Barbalha and Crato (RR95 ≥ 30) imply a reduction of temephos efficacy, and indeed in simulated field tests reduced effectiveness was observed for the Barbalha population. Two populations (Crato and Barbalha) were also resistant to cypermethrin, whilst Juazeiro do Norte showed only an altered susceptibility. The Ile1011Met kdr mutation was detected in all three populations and Val1016Ile in Crato and Juazeiro do Norte. 1011Met was significantly associated with resistance to cypermethrin in the Crato population. Biochemical tests showed that only the activity of esterases and GSTs, among the tested detoxification enzymes, was altered in these populations when compared with the Rockefeller strain. Conclusions Our results demonstrate that two A. aegypti populations from Ceará are under strong selection pressure by temephos, compromising the field effectiveness of this organophosphate. Our results also provide evidence that the process of reducing resistance to this larvicide in the field is difficult and slow and may require more than seven years for reversal. In addition, we show resistance to cypermethrin in two of the three populations studied, and for the first time the presence of the allele 1016Ile in mosquito populations from northeastern Brazil. A significant association between 1011M et and resistance was observed in one of the populations. Target-site mechanisms seem not to be implicated in temephos resistance, reinforcing the idea that for the studied populations, detoxification enzymes most likely play a major role in the resistance to this insecticide.engInsecticide resistance in Aedes aegypti populations from Ceará, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleResistance LevelCypermethrinDengue Hemorrhagic FeverResistance RatioMosquito PopulationAedes / efeitos de drogasAedes / genéticaAlelosAnimaisBrasilVetores de doençasFêmeaProteínas de Insetos / genéticaResistência a inseticidasInseticidas / farmacologiaMasculinoMutação, MissensePiretrinas / farmacologiaSeleção GenéticaTemefós / farmacologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83094https://www.arca.fiocruz.br/bitstream/icict/31254/1/license.txtee3692f7b0b97d5638099ab94397d05fMD51ORIGINALInsecticide resistance in Aedes aegypti populations from Ceará, Brazil.pdfInsecticide resistance in Aedes aegypti populations from Ceará, Brazil.pdfapplication/pdf893212https://www.arca.fiocruz.br/bitstream/icict/31254/2/Insecticide%20resistance%20in%20Aedes%20aegypti%20populations%20from%20Cear%c3%a1%2c%20Brazil.pdf29595e56eaeb68cafd5b9e0e7f8cf9f8MD52TEXTInsecticide resistance in Aedes aegypti populations from Ceará, Brazil.pdf.txtInsecticide resistance in Aedes aegypti populations from Ceará, Brazil.pdf.txtExtracted texttext/plain57276https://www.arca.fiocruz.br/bitstream/icict/31254/3/Insecticide%20resistance%20in%20Aedes%20aegypti%20populations%20from%20Cear%c3%a1%2c%20Brazil.pdf.txt6ce762009a88798b2ec3afa311a00cd5MD53icict/312542019-01-24 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| dc.title.pt_BR.fl_str_mv |
Insecticide resistance in Aedes aegypti populations from Ceará, Brazil |
| title |
Insecticide resistance in Aedes aegypti populations from Ceará, Brazil |
| spellingShingle |
Insecticide resistance in Aedes aegypti populations from Ceará, Brazil Lima, Estelita Pereira Resistance Level Cypermethrin Dengue Hemorrhagic Fever Resistance Ratio Mosquito Population Aedes / efeitos de drogas Aedes / genética Alelos Animais Brasil Vetores de doenças Fêmea Proteínas de Insetos / genética Resistência a inseticidas Inseticidas / farmacologia Masculino Mutação, Missense Piretrinas / farmacologia Seleção Genética Temefós / farmacologia |
| title_short |
Insecticide resistance in Aedes aegypti populations from Ceará, Brazil |
| title_full |
Insecticide resistance in Aedes aegypti populations from Ceará, Brazil |
| title_fullStr |
Insecticide resistance in Aedes aegypti populations from Ceará, Brazil |
| title_full_unstemmed |
Insecticide resistance in Aedes aegypti populations from Ceará, Brazil |
| title_sort |
Insecticide resistance in Aedes aegypti populations from Ceará, Brazil |
| author |
Lima, Estelita Pereira |
| author_facet |
Lima, Estelita Pereira Paiva, Marcelo Henrique Santos Araújo, Ana Paula de Silva, Ellyda Vanessa Gomes da Silva, Ulisses Mariano da Oliveira, Lúcia Nogueira de Santana, Antonio Euzébio G. Barbosa, Clarisse Nogueira Paiva Neto, Clovis C. de Goulart, Marilia O. F. Wilding, Craig Stephen Ayres, Constância Flávia Junqueira Santos, Maria Alice V. de Melo |
| author_role |
author |
| author2 |
Paiva, Marcelo Henrique Santos Araújo, Ana Paula de Silva, Ellyda Vanessa Gomes da Silva, Ulisses Mariano da Oliveira, Lúcia Nogueira de Santana, Antonio Euzébio G. Barbosa, Clarisse Nogueira Paiva Neto, Clovis C. de Goulart, Marilia O. F. Wilding, Craig Stephen Ayres, Constância Flávia Junqueira Santos, Maria Alice V. de Melo |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lima, Estelita Pereira Paiva, Marcelo Henrique Santos Araújo, Ana Paula de Silva, Ellyda Vanessa Gomes da Silva, Ulisses Mariano da Oliveira, Lúcia Nogueira de Santana, Antonio Euzébio G. Barbosa, Clarisse Nogueira Paiva Neto, Clovis C. de Goulart, Marilia O. F. Wilding, Craig Stephen Ayres, Constância Flávia Junqueira Santos, Maria Alice V. de Melo |
| dc.subject.en.pt_BR.fl_str_mv |
Resistance Level Cypermethrin Dengue Hemorrhagic Fever Resistance Ratio Mosquito Population |
| topic |
Resistance Level Cypermethrin Dengue Hemorrhagic Fever Resistance Ratio Mosquito Population Aedes / efeitos de drogas Aedes / genética Alelos Animais Brasil Vetores de doenças Fêmea Proteínas de Insetos / genética Resistência a inseticidas Inseticidas / farmacologia Masculino Mutação, Missense Piretrinas / farmacologia Seleção Genética Temefós / farmacologia |
| dc.subject.decs.pt_BR.fl_str_mv |
Aedes / efeitos de drogas Aedes / genética Alelos Animais Brasil Vetores de doenças Fêmea Proteínas de Insetos / genética Resistência a inseticidas Inseticidas / farmacologia Masculino Mutação, Missense Piretrinas / farmacologia Seleção Genética Temefós / farmacologia |
| description |
National Council for Scientific and Technological Development (CNPq, Grants ID: 479825/2009-2 and 550116/2010-9), CAPES/PROCAD/NF 23, Rede Nordeste de Biotecnologia (RENORBIO) and Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE, Grant ID: APQ 1361-4.00/08). |
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2011 |
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2011 |
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2019-01-23T14:29:11Z |
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2019-01-23T14:29:11Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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LIMA, Estelita Pereira et al. Insecticide Resistance in Aedes Aegypti Populations from Ceará, Brazil. Parasites & Vectors, v. 4, p. 5, 12 jan. 2011. |
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https://www.arca.fiocruz.br/handle/icict/31254 |
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1756-3305 |
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10.1186/1756-3305-4-5 |
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LIMA, Estelita Pereira et al. Insecticide Resistance in Aedes Aegypti Populations from Ceará, Brazil. Parasites & Vectors, v. 4, p. 5, 12 jan. 2011. 1756-3305 10.1186/1756-3305-4-5 |
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