Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study

Detalhes bibliográficos
Autor(a) principal: Martins, Ezequias B.
Data de Publicação: 2022
Outros Autores: Silva, Michele F. B., Tassinari, Wagner S., Nogueira, Fernanda de Bruycker, Moraes, Isabella C. V., Rodrigues, Cintia D. S., Santos, Carolina C., Sampaio, Simone A., Costa, Anielle Pina, Fabri, Allison A., Campos, Vinícius Guerra, Santos, Nayara A., Faria, Nieli R. C., Filippis, Ana Maria B., Brasil, Patrícia, Calvet, Guilherme A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56558
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.
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spelling Martins, Ezequias B.Silva, Michele F. B.Tassinari, Wagner S.Nogueira, Fernanda de BruyckerMoraes, Isabella C. V.Rodrigues, Cintia D. S.Santos, Carolina C.Sampaio, Simone A.Costa, Anielle PinaFabri, Allison A.Campos, Vinícius GuerraSantos, Nayara A.Faria, Nieli R. C.Filippis, Ana Maria B.Brasil, PatríciaCalvet, Guilherme A.2023-01-23T16:30:07Z2023-01-23T16:30:07Z2022MARTINS, Ezequias B. et al. Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study. PLoS Negl Trop Dis. v. 16, n. 3, e0010242, p. 1 - 16, Mar. 2022.1935-2727https://www.arca.fiocruz.br/handle/icict/5655810.1371/journal.pntd.0010242engPublic Library of ScienceVírus da ChikungunyaDetecção do vírus em fluidosEstudo de coorte INOVACHIKChikungunya virusThe INOVACHIK cohort studyDetection virus in bodily fluidsDetection of Chikungunya virus in bodily fluids: The INOVACHIK cohort studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Ciências Exatas. Departamento de Matemática. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.Background Chikungunya is a widely distributed, re-emerging tropical disease caused by the chikungunya virus (CHIKV). Little is known about the duration for which CHIK RNA are detectable in bodily fluids, especially genital secretions, and current evidence is based on small series or case reports. An understanding of viral dynamics across different body compartments can inform diagnostic testing algorithms and public health prevention interventions. Methodology A prospective cohort study was conducted to assess the presence and duration of detectable levels of CHIKV RNA in blood, urine, saliva, semen, and vaginal secretions. Men and women (� 18 years) with a positive reverse transcriptase-polymerase chain reaction (RT-PCR) test for CHIKV in the acute phase (1–14 days) of the disease were included. After enrollment, clinical data and samples were collected every 15 days over the first 2 months, and a final collection was performed 3 months after recruitment. The Kaplan–Meier interval-censoring method and the parametric Weibull model were fitted to estimate the median time of viral persistence until the lack of CHIKV RNA detection among all body fluids. Punctual estimates of the median time of CHIKV RNA persistence for each fluid were estimated using a 95% confidence interval (CI). Results From April to December 2019, 170 participants were screened. Of these, 152 (100 women) were enrolled in the study. The median and interquartile range (IQR) ages for men and women were 39.3 (IQR: 26.9, 50.7) and 43.5 (IQR: 33.8, 53.6) years, respectively. CHIKV RNA was detected in 80.3% (122/152) of serum samples, 23.0% (35/152) of urine samples, 30.3% (46/152) of saliva samples, 14.3% (6/42) of semen samples, and 20.2% (20/99) of vaginal secretion samples. The median time until the loss of CHIKV RNA detection was 19.6 days (95% CI, 17.5–21.7) in serum, 25.3 days (95% CI, 17.8–32.8) in urine, 23.1 days (95% CI, 17.9–28.4) in saliva, and 25.8 days (95% CI, 20.6–31.1) in vaginal secretion. The number of semen samples available was too small to make statistical estimates, but a last positive sample was obtained from a participant 56 days after the onset of symptoms. Conclusions CHIKV RNA could be detected in all bodily fluids studied, including genital secretions during the acute and convalescent phases and additional studies on viral infectivity in semen and vaginal secretions are warranted.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56558/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAnaMaria Bispodefilipis_etal_IOC_2022.pdfAnaMaria Bispodefilipis_etal_IOC_2022.pdfapplication/pdf1629313https://www.arca.fiocruz.br/bitstream/icict/56558/2/AnaMaria%20Bispodefilipis_etal_IOC_2022.pdf57c18f014fa7eb5bfeba5cdcb8b8e972MD52icict/565582023-09-04 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dc.title.en_US.fl_str_mv Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study
title Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study
spellingShingle Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study
Martins, Ezequias B.
Vírus da Chikungunya
Detecção do vírus em fluidos
Estudo de coorte INOVACHIK
Chikungunya virus
The INOVACHIK cohort study
Detection virus in bodily fluids
title_short Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study
title_full Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study
title_fullStr Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study
title_full_unstemmed Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study
title_sort Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study
author Martins, Ezequias B.
author_facet Martins, Ezequias B.
Silva, Michele F. B.
Tassinari, Wagner S.
Nogueira, Fernanda de Bruycker
Moraes, Isabella C. V.
Rodrigues, Cintia D. S.
Santos, Carolina C.
Sampaio, Simone A.
Costa, Anielle Pina
Fabri, Allison A.
Campos, Vinícius Guerra
Santos, Nayara A.
Faria, Nieli R. C.
Filippis, Ana Maria B.
Brasil, Patrícia
Calvet, Guilherme A.
author_role author
author2 Silva, Michele F. B.
Tassinari, Wagner S.
Nogueira, Fernanda de Bruycker
Moraes, Isabella C. V.
Rodrigues, Cintia D. S.
Santos, Carolina C.
Sampaio, Simone A.
Costa, Anielle Pina
Fabri, Allison A.
Campos, Vinícius Guerra
Santos, Nayara A.
Faria, Nieli R. C.
Filippis, Ana Maria B.
Brasil, Patrícia
Calvet, Guilherme A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Martins, Ezequias B.
Silva, Michele F. B.
Tassinari, Wagner S.
Nogueira, Fernanda de Bruycker
Moraes, Isabella C. V.
Rodrigues, Cintia D. S.
Santos, Carolina C.
Sampaio, Simone A.
Costa, Anielle Pina
Fabri, Allison A.
Campos, Vinícius Guerra
Santos, Nayara A.
Faria, Nieli R. C.
Filippis, Ana Maria B.
Brasil, Patrícia
Calvet, Guilherme A.
dc.subject.other.en_US.fl_str_mv Vírus da Chikungunya
Detecção do vírus em fluidos
Estudo de coorte INOVACHIK
topic Vírus da Chikungunya
Detecção do vírus em fluidos
Estudo de coorte INOVACHIK
Chikungunya virus
The INOVACHIK cohort study
Detection virus in bodily fluids
dc.subject.en.en_US.fl_str_mv Chikungunya virus
The INOVACHIK cohort study
Detection virus in bodily fluids
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-01-23T16:30:07Z
dc.date.available.fl_str_mv 2023-01-23T16:30:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv MARTINS, Ezequias B. et al. Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study. PLoS Negl Trop Dis. v. 16, n. 3, e0010242, p. 1 - 16, Mar. 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56558
dc.identifier.issn.en_US.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0010242
identifier_str_mv MARTINS, Ezequias B. et al. Detection of Chikungunya virus in bodily fluids: The INOVACHIK cohort study. PLoS Negl Trop Dis. v. 16, n. 3, e0010242, p. 1 - 16, Mar. 2022.
1935-2727
10.1371/journal.pntd.0010242
url https://www.arca.fiocruz.br/handle/icict/56558
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Public Library of Science
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