Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11835 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. |
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Temerozo, Jairo R.Joaquim, RafaelRegis, Eduardo G.Savino, WilsonBou-Habib, Dumith Chequer2015-09-28T13:02:39Z2015-09-28T13:02:39Z2013TEMEROZO, Jairo R.; et al. Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP. Plos One, v.8, n.6, e67701, 8p, June 2013.https://www.arca.fiocruz.br/handle/icict/1183510.1371/journal.pone.0067701engPlos OneMacrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAPinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Central Hospital of Maputo. Laboratory of Microbiology. Maputo, Mozambique.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.It is well established that host factors can modulate HIV-1 replication in macrophages, critical cells in the pathogenesis of HIV-1 infection due to their ability to continuously produce virus. The neuropeptides VIP and PACAP induce wellcharacterized effects on macrophages through binding to the G protein-coupled receptors VPAC1, VPAC2 and PAC1, but their influence on HIV-1 production by these cells has not been established. Here, we describe that VIP and PACAP reduce macrophage production of HIV-1, acting in a synergistic or additive manner to decrease viral growth. Using receptor antagonists, we detected that the HIV-1 inhibition promoted by VIP is dependent on its ligation to VPAC1/2, whereas PACAP decreases HIV-1 growth via activation of the VPAC1/2 and PAC1 receptors. Specific agonists of VPAC2 or PAC1 decrease macrophage production of HIV-1, whereas sole activation of VPAC1 enhances viral growth. However, the combination of specific agonists mimicking the receptor preference of the natural neuropeptides reproduces the ability of VIP and PACAP to increase macrophage resistance to HIV-1 replication. VIP and PACAP up-regulated macrophage secretion of the b-chemokines CCL3 and CCL5 and the cytokine IL-10, whose neutralization reversed the neuropeptide-induced inhibition of HIV-1 replication. Our results suggest that VIP and PACAP and the receptors VPAC2 and PAC1 could be used as targets for developing alternative therapeutic strategies for HIV-1 infection.HIV-1 InfectionMacrophagePACAPVIPNeuropeptidesNeuropeptídeosMacrófagosInfecções por HIVReceptores de Polipeptídeo Hipofisário Ativador de Adenilato CiclaseReceptores de Peptídeo Intestinal Vasoativoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11835/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALjairo_temerozo_etal_IOC_2013.pdfapplication/pdf786578https://www.arca.fiocruz.br/bitstream/icict/11835/2/jairo_temerozo_etal_IOC_2013.pdf9868230f07b2fe1723aac22a44466825MD52TEXTjairo_temerozo_etal_IOC_2013.pdf.txtjairo_temerozo_etal_IOC_2013.pdf.txtExtracted texttext/plain42164https://www.arca.fiocruz.br/bitstream/icict/11835/3/jairo_temerozo_etal_IOC_2013.pdf.txt5186e4c3d36e1f312e0c3f964a3ec10cMD53icict/118352022-06-24 13:11:05.557oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:11:05Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP |
| title |
Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP |
| spellingShingle |
Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP Temerozo, Jairo R. HIV-1 Infection Macrophage PACAP VIP Neuropeptides Neuropeptídeos Macrófagos Infecções por HIV Receptores de Polipeptídeo Hipofisário Ativador de Adenilato Ciclase Receptores de Peptídeo Intestinal Vasoativo |
| title_short |
Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP |
| title_full |
Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP |
| title_fullStr |
Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP |
| title_full_unstemmed |
Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP |
| title_sort |
Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP |
| author |
Temerozo, Jairo R. |
| author_facet |
Temerozo, Jairo R. Joaquim, Rafael Regis, Eduardo G. Savino, Wilson Bou-Habib, Dumith Chequer |
| author_role |
author |
| author2 |
Joaquim, Rafael Regis, Eduardo G. Savino, Wilson Bou-Habib, Dumith Chequer |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Temerozo, Jairo R. Joaquim, Rafael Regis, Eduardo G. Savino, Wilson Bou-Habib, Dumith Chequer |
| dc.subject.en.pt_BR.fl_str_mv |
HIV-1 Infection Macrophage PACAP VIP Neuropeptides |
| topic |
HIV-1 Infection Macrophage PACAP VIP Neuropeptides Neuropeptídeos Macrófagos Infecções por HIV Receptores de Polipeptídeo Hipofisário Ativador de Adenilato Ciclase Receptores de Peptídeo Intestinal Vasoativo |
| dc.subject.decs.pt_BR.fl_str_mv |
Neuropeptídeos Macrófagos Infecções por HIV Receptores de Polipeptídeo Hipofisário Ativador de Adenilato Ciclase Receptores de Peptídeo Intestinal Vasoativo |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. |
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2013 |
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2013 |
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2015-09-28T13:02:39Z |
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2015-09-28T13:02:39Z |
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TEMEROZO, Jairo R.; et al. Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP. Plos One, v.8, n.6, e67701, 8p, June 2013. |
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TEMEROZO, Jairo R.; et al. Macrophage Resistance to HIV-1 Infection Is Enhanced by the Neuropeptides VIP and PACAP. Plos One, v.8, n.6, e67701, 8p, June 2013. 10.1371/journal.pone.0067701 |
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