Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction

Detalhes bibliográficos
Autor(a) principal: Silva, Tatiana Araújo
Data de Publicação: 2019
Outros Autores: Ferreira, Luis Felipe de Carvalho, Pereira, Mirian Claudia de Souza, Calvet, Claudia Magalhães
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/41093
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.
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spelling Silva, Tatiana AraújoFerreira, Luis Felipe de CarvalhoPereira, Mirian Claudia de SouzaCalvet, Claudia Magalhães2020-05-04T19:03:50Z2020-05-04T19:03:50Z2019SILVA, Tatiana Araújo et al. Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction. International Journal of Molecular Sciences, v. 20, n. 4836, p. 1-21, 2019.1661-6596https://www.arca.fiocruz.br/handle/icict/4109310.3390/ijms201948361422-0067engMDPITrypanosoma cruziTGF-βFibrose cardíacaMatriz extracelularCaminhos de sinalizaçãoTrypanosoma cruziTGF-βHeart fibrosisExtracellular matrixSignaling pathwaysSMAD2p38 MAPKc-JunDifferential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interactioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.Transforming growth factor beta (TGF-β) is a determinant for inflammation and fibrosis in cardiac and skeletal muscle in Chagas disease. To determine its regulatory mechanisms, we investigated the response of Trypanosoma cruzi-infected cardiomyocytes (CM), cardiac fibroblasts (CF), and L6E9 skeletal myoblasts to TGF-β. Cultures of CM, CF, and L6E9 were infected with T. cruzi (Y strain) and treated with TGF-β (1-10 ng/mL, 1 h or 48 h). Fibronectin (FN) distribution was analyzed by immunofluorescence and Western blot (WB). Phosphorylated SMAD2 (PS2), phospho-p38 (p-p38), and phospho-c-Jun (p-c-Jun) signaling were evaluated by WB. CF and L6E9 showed an increase in FN from 1 ng/mL of TGF-β, while CM displayed FN modulation only after 10 ng/mL treatment. CF and L6E9 showed higher PS2 levels than CM, while p38 was less stimulated in CF than CM and L6E9. T. cruzi infection resulted in localized FN disorganization in CF and L6E9. T. cruzi induced an increase in FN in CF cultures, mainly in uninfected cells. Infected CF cultures treated with TGF-β showed a reduction in PS2 and an increase in p-p38 and p-c-Jun levels. Our data suggest that p38 and c-Jun pathways may be participating in the fibrosis regulatory process mediated by TGF-β after T. cruzi infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41093/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMirianCSPereira_etal_IOC_2019.pdfMirianCSPereira_etal_IOC_2019.pdfapplication/pdf4168011https://www.arca.fiocruz.br/bitstream/icict/41093/2/MirianCSPereira_etal_IOC_2019.pdfe2467c33bb424e1d0cf51f2d047adac7MD52TEXTMirianCSPereira_etal_IOC_2019.pdf.txtMirianCSPereira_etal_IOC_2019.pdf.txtExtracted texttext/plain87985https://www.arca.fiocruz.br/bitstream/icict/41093/3/MirianCSPereira_etal_IOC_2019.pdf.txtbb2f6178ca2141f5eff8325bf31dfab8MD53icict/410932022-06-24 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dc.title.pt_BR.fl_str_mv Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction
title Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction
spellingShingle Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction
Silva, Tatiana Araújo
Trypanosoma cruzi
TGF-β
Fibrose cardíaca
Matriz extracelular
Caminhos de sinalização
Trypanosoma cruzi
TGF-β
Heart fibrosis
Extracellular matrix
Signaling pathways
SMAD2
p38 MAPK
c-Jun
title_short Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction
title_full Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction
title_fullStr Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction
title_full_unstemmed Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction
title_sort Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction
author Silva, Tatiana Araújo
author_facet Silva, Tatiana Araújo
Ferreira, Luis Felipe de Carvalho
Pereira, Mirian Claudia de Souza
Calvet, Claudia Magalhães
author_role author
author2 Ferreira, Luis Felipe de Carvalho
Pereira, Mirian Claudia de Souza
Calvet, Claudia Magalhães
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva, Tatiana Araújo
Ferreira, Luis Felipe de Carvalho
Pereira, Mirian Claudia de Souza
Calvet, Claudia Magalhães
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
TGF-β
Fibrose cardíaca
Matriz extracelular
Caminhos de sinalização
topic Trypanosoma cruzi
TGF-β
Fibrose cardíaca
Matriz extracelular
Caminhos de sinalização
Trypanosoma cruzi
TGF-β
Heart fibrosis
Extracellular matrix
Signaling pathways
SMAD2
p38 MAPK
c-Jun
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
TGF-β
Heart fibrosis
Extracellular matrix
Signaling pathways
SMAD2
p38 MAPK
c-Jun
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-05-04T19:03:50Z
dc.date.available.fl_str_mv 2020-05-04T19:03:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Tatiana Araújo et al. Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction. International Journal of Molecular Sciences, v. 20, n. 4836, p. 1-21, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/41093
dc.identifier.issn.pt_BR.fl_str_mv 1661-6596
dc.identifier.doi.none.fl_str_mv 10.3390/ijms20194836
dc.identifier.eissn.none.fl_str_mv 1422-0067
identifier_str_mv SILVA, Tatiana Araújo et al. Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction. International Journal of Molecular Sciences, v. 20, n. 4836, p. 1-21, 2019.
1661-6596
10.3390/ijms20194836
1422-0067
url https://www.arca.fiocruz.br/handle/icict/41093
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/41093/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/41093/2/MirianCSPereira_etal_IOC_2019.pdf
https://www.arca.fiocruz.br/bitstream/icict/41093/3/MirianCSPereira_etal_IOC_2019.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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