Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/39722 |
Resumo: | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Interações Célula-Célula. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Pós-graduação em Parasitologia. Belo Horizonte, MG, Brasil. |
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Passos, Livia Silva AraújoMagalhães, Luísa Mourão DiasSoares, Rodrigo Pedro PintoMarques, Alexandre F.Alves, Marina Luiza RodriguesGiunchetti, Rodolfo CordeiroNunes, Maria do Carmo PereiraGollob, Kenneth J.Dutra, Walderez Ornelas2020-02-04T16:49:01Z2020-02-04T16:49:01Z2019PASSOS, Livia Silva Araújo et al. Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease. Frontiers in Immunology, v. 10, p. 1-14, 04 Jan. 2019.1664-3224https://www.arca.fiocruz.br/handle/icict/3972210.3389/fimmu.2018.03015engFrontiers Research FoundationActivation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Interações Célula-Célula. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Pós-graduação em Parasitologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Interações Célula-Célula. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Pós-graduação em Parasitologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Pós-graduação em Parasitologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Pós-graduação em Parasitologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Interações Célula-Célula. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Interações Célula-Célula. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Pós-graduação em Parasitologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte, MG, BrazilCentro de Pesquisa Internacional, A.C.Camargo Cancer Center, São Paulo, Brasil / Instituto Nacional de Ciência e Tecnologia Doenças Tropicais. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Interações Célula-Célula. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Pós-graduação em Parasitologia. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia Doenças Tropicais. Belo Horizonte, MG, Brasil.B-cells mediate humoral adaptive immune response via the production of antibodies and cytokines, and by inducing T-cell activation. These functions can be attributed to distinct B-cell subpopulations. Infection with Trypanosoma cruzi, the causative agent of Chagas disease, induces a polyclonal B-cell activation and lytic antibody production, critical for controlling parasitemia. Individuals within the chronic phase of Chagas disease may remain in an asymptomatic form (indeterminate), or develop severe cardiomyopathy (cardiac form) that can lead to death. Currently, there is no effective vaccine to prevent Chagas disease, and no treatment to halt the development of the cardiomyopathy once it is installed. The pathology associated with cardiac Chagas disease is a result of an inflammatory reaction. Thus, discovering characteristics of the host's immune response that favor the maintenance of favorable heart function may unveil important immunotherapeutic targets. Given the importance of B cells in antibody production and parasite control, we investigated T. cruzi-derived antigenic fractions responsible for B-cell activation and whether frequencies and functional characteristics of B-cell subpopulations are associated with different clinical outcomes of human Chagas disease. We stimulated cells from indeterminate (I) and cardiac (C) Chagas patients, as well as non-infected individuals (NI), with T. cruzi-derived protein- (PRO), glycolipid- (GCL) and lipid (LIP)-enriched fractions and determined functional characteristics of B-cell subpopulations. Our results showed that the frequency of B-cells was similar amongst groups. PRO, but not GCL nor LIP, led to an increased frequency of B1 B-cells in I, but not C nor NI. Although stimulation with PRO induced higher TNF expression by B1 B-cells from C and I, as compared to NI, it induced expression of IL-10 in cells from I, but not C. Stimulation with PRO induced an increased frequency of the CD11b+ B1 B-cell subpopulation, which was associated with better cardiac function. Chagas patients displayed increased IgM production, and activation of gamma-delta T-cells, which have been associated with B1 B-cell function. Our data showed that PRO activates CD11b+ B1 B-cells, and that this activation is associated with a beneficial clinical status. These findings may have implications in designing new strategies focusing on B-cell activation to prevent Chagas disease cardiomyopathy.B1 B-cellsChagas diseaseCardiomyopathyTrypanosoma-cruziImmunoregulationCytokinesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/39722/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALActivation of Human CD11b+.pdfActivation of Human CD11b+.pdfapplication/pdf8093089https://www.arca.fiocruz.br/bitstream/icict/39722/2/Activation%20of%20Human%20CD11b%2b.pdfcd208b21b78625963320dab6d515f28fMD52TEXTActivation of Human CD11b+.pdf.txtActivation of Human CD11b+.pdf.txtExtracted texttext/plain14https://www.arca.fiocruz.br/bitstream/icict/39722/3/Activation%20of%20Human%20CD11b%2b.pdf.txt06b7e51e8fc077b8c75076712e4dd2b3MD53icict/397222020-02-07 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| dc.title.pt_BR.fl_str_mv |
Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease |
| title |
Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease |
| spellingShingle |
Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease Passos, Livia Silva Araújo B1 B-cells Chagas disease Cardiomyopathy Trypanosoma-cruzi Immunoregulation Cytokines |
| title_short |
Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease |
| title_full |
Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease |
| title_fullStr |
Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease |
| title_full_unstemmed |
Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease |
| title_sort |
Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease |
| author |
Passos, Livia Silva Araújo |
| author_facet |
Passos, Livia Silva Araújo Magalhães, Luísa Mourão Dias Soares, Rodrigo Pedro Pinto Marques, Alexandre F. Alves, Marina Luiza Rodrigues Giunchetti, Rodolfo Cordeiro Nunes, Maria do Carmo Pereira Gollob, Kenneth J. Dutra, Walderez Ornelas |
| author_role |
author |
| author2 |
Magalhães, Luísa Mourão Dias Soares, Rodrigo Pedro Pinto Marques, Alexandre F. Alves, Marina Luiza Rodrigues Giunchetti, Rodolfo Cordeiro Nunes, Maria do Carmo Pereira Gollob, Kenneth J. Dutra, Walderez Ornelas |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Passos, Livia Silva Araújo Magalhães, Luísa Mourão Dias Soares, Rodrigo Pedro Pinto Marques, Alexandre F. Alves, Marina Luiza Rodrigues Giunchetti, Rodolfo Cordeiro Nunes, Maria do Carmo Pereira Gollob, Kenneth J. Dutra, Walderez Ornelas |
| dc.subject.en.pt_BR.fl_str_mv |
B1 B-cells Chagas disease Cardiomyopathy Trypanosoma-cruzi Immunoregulation Cytokines |
| topic |
B1 B-cells Chagas disease Cardiomyopathy Trypanosoma-cruzi Immunoregulation Cytokines |
| description |
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Interações Célula-Célula. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Pós-graduação em Parasitologia. Belo Horizonte, MG, Brasil. |
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2019 |
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2019 |
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2020-02-04T16:49:01Z |
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2020-02-04T16:49:01Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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PASSOS, Livia Silva Araújo et al. Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease. Frontiers in Immunology, v. 10, p. 1-14, 04 Jan. 2019. |
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https://www.arca.fiocruz.br/handle/icict/39722 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2018.03015 |
| identifier_str_mv |
PASSOS, Livia Silva Araújo et al. Activation of Human CD11b+ B1 B-Cells by Trypanosoma cruzi-Derived Proteins Is Associated With Protective Immune Response in Human Chagas Disease. Frontiers in Immunology, v. 10, p. 1-14, 04 Jan. 2019. 1664-3224 10.3389/fimmu.2018.03015 |
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Frontiers Research Foundation |
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Frontiers Research Foundation |
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