Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake

Detalhes bibliográficos
Autor(a) principal: Rugani, Jeronimo Nunes
Data de Publicação: 2019
Outros Autores: Gontijo, Célia Maria Ferreira, Frézard, Frédéric, Soares, Rodrigo Pedro Pinto, Monte Neto, Rubens Lima do
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39798
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
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spelling Rugani, Jeronimo NunesGontijo, Célia Maria FerreiraFrézard, FrédéricSoares, Rodrigo Pedro PintoMonte Neto, Rubens Lima do2020-02-07T16:12:46Z2020-02-07T16:12:46Z2019RUGANI, Jeronimo Nunes et al. Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-9, 2019.0102-311Xhttps://www.arca.fiocruz.br/handle/icict/3979810.1590/0074-02760190111engFundação Oswaldo CruzAntimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptakeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.BACKGROUND: In addition to the limited therapeutic arsenal and the side effects of antileishmanial agents, drug resistance hinders disease control. In Brazil, Leishmania braziliensis causes atypical (AT) tegumentary leishmaniasis lesions, frequently refractory to treatment. OBJECTIVES: The main goal of this study was to characterise antimony (Sb)-resistant (SbR) L. braziliensis strains obtained from patients living in Xakriabá indigenous community, Minas Gerais, Brazil. METHODS: The aquaglyceroporin 1-encoding gene (AQP1) from L. braziliensis clinical isolates was sequenced, and its function was evaluated by hypo-osmotic shock. mRNA levels of genes associated with Sb resistance were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Atomic absorption was used to measure Sb uptake. FINDINGS: Although clinical isolates presented delayed recovery time in hypo-osmotic shock, AQP1 function was maintained. Isolate 340 accumulated less Sb than all other isolates, supporting the 65-fold downregulation of AQP1 mRNA levels. Both 330 and 340 isolates upregulated antimony resistance marker (ARM) 56/ARM58 and multidrug resistant protein A (MRPA); however, only ARM58 upregulation was an exclusive feature of SbR field isolates. CA7AE seemed to increase drug uptake in L. braziliensis and represented a tool to study the role of glycoconjugates in Sb transport. MAIN CONCLUSIONS: There is a clear correlation between ARM56/58 upregulation and Sb resistance in AT-harbouring patients, suggesting the use of these markers as potential indicators to help the treatment choice and outcome, preventing therapeutic failure.Antimony resistanceLeishmania braziliensisField isolatesAntimony resistance markerARM56ARM58Atypical lesionsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/39798/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALAntimony resistance in Leishmania.pdfAntimony resistance in Leishmania.pdfapplication/pdf8713414https://www.arca.fiocruz.br/bitstream/icict/39798/2/Antimony%20resistance%20in%20Leishmania.pdf62fcd0e321200e1fe1c99d65d95a214bMD52TEXTAntimony resistance in Leishmania.pdf.txtAntimony resistance in Leishmania.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake
title Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake
spellingShingle Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake
Rugani, Jeronimo Nunes
Antimony resistance
Leishmania braziliensis
Field isolates
Antimony resistance marker
ARM56
ARM58
Atypical lesions
title_short Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake
title_full Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake
title_fullStr Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake
title_full_unstemmed Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake
title_sort Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake
author Rugani, Jeronimo Nunes
author_facet Rugani, Jeronimo Nunes
Gontijo, Célia Maria Ferreira
Frézard, Frédéric
Soares, Rodrigo Pedro Pinto
Monte Neto, Rubens Lima do
author_role author
author2 Gontijo, Célia Maria Ferreira
Frézard, Frédéric
Soares, Rodrigo Pedro Pinto
Monte Neto, Rubens Lima do
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rugani, Jeronimo Nunes
Gontijo, Célia Maria Ferreira
Frézard, Frédéric
Soares, Rodrigo Pedro Pinto
Monte Neto, Rubens Lima do
dc.subject.en.pt_BR.fl_str_mv Antimony resistance
Leishmania braziliensis
Field isolates
Antimony resistance marker
ARM56
ARM58
Atypical lesions
topic Antimony resistance
Leishmania braziliensis
Field isolates
Antimony resistance marker
ARM56
ARM58
Atypical lesions
description Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-02-07T16:12:46Z
dc.date.available.fl_str_mv 2020-02-07T16:12:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RUGANI, Jeronimo Nunes et al. Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-9, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39798
dc.identifier.issn.pt_BR.fl_str_mv 0102-311X
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760190111
identifier_str_mv RUGANI, Jeronimo Nunes et al. Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-9, 2019.
0102-311X
10.1590/0074-02760190111
url https://www.arca.fiocruz.br/handle/icict/39798
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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