Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Christiana Vargas
Data de Publicação: 2019
Outros Autores: Rocha, Bruna Fonte Boa, Moreira, Douglas de Souza, Pascoal, Vanessa Peruhype Magalhães, Murta, Silvane Maria Fonseca
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36577
Resumo: Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.
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spelling Ribeiro, Christiana VargasRocha, Bruna Fonte BoaMoreira, Douglas de SouzaPascoal, Vanessa Peruhype MagalhãesMurta, Silvane Maria Fonseca2019-10-18T17:10:16Z2019-10-18T17:10:16Z2019RIBEIRO, Christiana Vargas et al. Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony. Parasites & Vectors, v. 12, p. 1-7, 2019.1756-3305https://www.arca.fiocruz.br/handle/icict/3657710.1371/journal.pone.0211374engBioMed CentralMannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimonyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.Background: Glycosylphosphatidylinositol is a surface molecule important for host-parasite interactions. Mannosyltransferase (GPI-14) is an essential enzyme for adding mannose on the glycosylphosphatidyl group. This study attempted to overexpress the GPI-14 gene in Leishmania braziliensis to investigate its role in the antimony-resistance phenotype of this parasite. Results: GPI-14 mRNA levels determined by quantitative real-time PCR (qRT-PCR) showed an increased expression in clones transfected with GPI-14 compared to its respective wild-type line. In order to investigate the expression profile of the surface carbohydrates of these clones, the intensity of the fluorescence emitted by the parasites after concanavalin-A (a lectin that binds to the terminal regions of α-D-mannosyl and α-D-glucosyl residues) treatment was analyzed. The results showed that the clones transfected with GPI-14 express 2.8-fold more mannose and glucose residues than those of the wild-type parental line, indicating effective GPI-14 overexpression. Antimony susceptibility tests using promastigotes showed that clones overexpressing the GPI-14 enzyme are 2.4- and 10.5-fold more resistant to potassium antimonyl tartrate (SbIII) than the parental non-transfected line. Infection analysis using THP-1 macrophages showed that amastigotes from both GPI-14 overexpressing clones were 3-fold more resistant to SbIII than the wild-type line. Conclusions: Our results suggest the involvement of the GPI-14 enzyme in the SbIII-resistance phenotype of L. braziliensis.Leishmania braziliensisAntimony-resistanceLeishmaniosesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/36577/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALMannosyltransferase (GPI-14).pdfMannosyltransferase (GPI-14).pdfapplication/pdf8148955https://www.arca.fiocruz.br/bitstream/icict/36577/2/Mannosyltransferase%20%28GPI-14%29.pdf222f5922140598b7f2eca54cf6c46c2dMD52TEXTMannosyltransferase (GPI-14).pdf.txtMannosyltransferase (GPI-14).pdf.txtExtracted texttext/plain24https://www.arca.fiocruz.br/bitstream/icict/36577/3/Mannosyltransferase%20%28GPI-14%29.pdf.txte93071b33a6014dfe257dac4d512fd09MD53icict/365772019-11-07 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dc.title.pt_BR.fl_str_mv Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony
title Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony
spellingShingle Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony
Ribeiro, Christiana Vargas
Leishmania braziliensis
Antimony-resistance
Leishmanioses
title_short Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony
title_full Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony
title_fullStr Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony
title_full_unstemmed Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony
title_sort Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony
author Ribeiro, Christiana Vargas
author_facet Ribeiro, Christiana Vargas
Rocha, Bruna Fonte Boa
Moreira, Douglas de Souza
Pascoal, Vanessa Peruhype Magalhães
Murta, Silvane Maria Fonseca
author_role author
author2 Rocha, Bruna Fonte Boa
Moreira, Douglas de Souza
Pascoal, Vanessa Peruhype Magalhães
Murta, Silvane Maria Fonseca
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Christiana Vargas
Rocha, Bruna Fonte Boa
Moreira, Douglas de Souza
Pascoal, Vanessa Peruhype Magalhães
Murta, Silvane Maria Fonseca
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Antimony-resistance
Leishmanioses
topic Leishmania braziliensis
Antimony-resistance
Leishmanioses
description Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-10-18T17:10:16Z
dc.date.available.fl_str_mv 2019-10-18T17:10:16Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RIBEIRO, Christiana Vargas et al. Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony. Parasites & Vectors, v. 12, p. 1-7, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36577
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0211374
identifier_str_mv RIBEIRO, Christiana Vargas et al. Mannosyltransferase (GPI-14) overexpression protects promastigote and amastigote forms of Leishmania braziliensis against trivalent antimony. Parasites & Vectors, v. 12, p. 1-7, 2019.
1756-3305
10.1371/journal.pone.0211374
url https://www.arca.fiocruz.br/handle/icict/36577
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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