The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia

Detalhes bibliográficos
Autor(a) principal: Santos, Marina Lima Silva
Data de Publicação: 2021
Outros Autores: Coimbra, Roney Santos, Sousa, Tais Nobrega de, Guimaraes, Luiz Felipe Ferreira, Gomes, Matheus de Souza, Amaral, Laurence Rodrigues do, Pereira, Dhelio Batista, Fontes, Cor Jesus Fernandes, Hawwari, Ibrahim, Franklin, Bernardo Simões, Carvalho, Luzia Helena
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51152
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil
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spelling Santos, Marina Lima SilvaCoimbra, Roney SantosSousa, Tais Nobrega deGuimaraes, Luiz Felipe FerreiraGomes, Matheus de SouzaAmaral, Laurence Rodrigues doPereira, Dhelio BatistaFontes, Cor Jesus FernandesHawwari, IbrahimFranklin, Bernardo SimõesCarvalho, Luzia Helena2022-02-11T12:42:41Z2022-02-11T12:42:41Z2021SANTOS, Marina Lima Silva et al. The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia. Front Cell Infect Microbiol., v. 11, 631333, 2021. doi: 10.3389/fcimb.2021.6313332235-2988https://www.arca.fiocruz.br/handle/icict/51152porFrontiers Media SAThe Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopeniainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, BrazilUniversidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, BrazilCentro de Pesquisas em Medicina Tropical. Dep. Pesquisa Clínica e Medicina Translacional. Porto Velho, RO. BrazilUniversidade Federal de Mato Grosso. Departamento de Clínica Médica. Cuiabá, MT, BrazilMedical Faculty. Institute of Innate Immunity. University of Bonn. Bonn, GermanyMedical Faculty. Institute of Innate Immunity. University of Bonn. Bonn, GermanyFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilSevere thrombocytopenia can be a determinant factor in the morbidity of Plasmodium vivax, the most widespread human malaria parasite. Although immune mechanisms may drive P. vivax-induced severe thrombocytopenia (PvST), the current data on the cytokine landscape in PvST is scarce and often conflicting. Here, we hypothesized that the analysis of the bidirectional circuit of inflammatory mediators and their regulatory miRNAs would lead to a better understanding of the mechanisms underlying PvST. For that, we combined Luminex proteomics, NanoString miRNA quantification, and machine learning to evaluate an extensive array of plasma mediators in uncomplicated P. vivax patients with different degrees of thrombocytopenia. Unsupervised clustering analysis identified a set of PvST-linked inflammatory (CXCL10, CCL4, and IL-18) and regulatory (IL- 10, IL-1Ra, HGF) mediators. Among the mediators associated with PvST, IL-6 and IL-8 were critical to discriminate P. vivax subgroups, while CCL2 and IFN-g from healthy controls. Supervised machine learning spotlighted IL-10 in P. vivax-mediated thrombocytopenia and provided evidence for a potential signaling route involving IL-8 and HGF. Finally, we identified a set of miRNAs capable of modulating these signaling pathways. In conclusion, the results place IL-10 and IL-8/HGF in the center of PvST and propose investigating these signaling pathways across the spectrum of malaria infections.Plasmodium vivaxmalariathrombocytopeniamiRNAcytokinechemokineinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51152/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALThe Interface Between Inflammatory Mediators.pdfThe Interface Between Inflammatory Mediators.pdfapplication/pdf737710https://www.arca.fiocruz.br/bitstream/icict/51152/2/The%20Interface%20Between%20Inflammatory%20Mediators.pdf483eeba7ebc623bebac847155004228aMD52icict/511522022-02-11 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dc.title.pt_BR.fl_str_mv The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia
title The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia
spellingShingle The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia
Santos, Marina Lima Silva
Plasmodium vivax
malaria
thrombocytopenia
miRNA
cytokine
chemokine
title_short The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia
title_full The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia
title_fullStr The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia
title_full_unstemmed The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia
title_sort The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia
author Santos, Marina Lima Silva
author_facet Santos, Marina Lima Silva
Coimbra, Roney Santos
Sousa, Tais Nobrega de
Guimaraes, Luiz Felipe Ferreira
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Pereira, Dhelio Batista
Fontes, Cor Jesus Fernandes
Hawwari, Ibrahim
Franklin, Bernardo Simões
Carvalho, Luzia Helena
author_role author
author2 Coimbra, Roney Santos
Sousa, Tais Nobrega de
Guimaraes, Luiz Felipe Ferreira
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Pereira, Dhelio Batista
Fontes, Cor Jesus Fernandes
Hawwari, Ibrahim
Franklin, Bernardo Simões
Carvalho, Luzia Helena
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Marina Lima Silva
Coimbra, Roney Santos
Sousa, Tais Nobrega de
Guimaraes, Luiz Felipe Ferreira
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Pereira, Dhelio Batista
Fontes, Cor Jesus Fernandes
Hawwari, Ibrahim
Franklin, Bernardo Simões
Carvalho, Luzia Helena
dc.subject.en.pt_BR.fl_str_mv Plasmodium vivax
malaria
thrombocytopenia
miRNA
cytokine
chemokine
topic Plasmodium vivax
malaria
thrombocytopenia
miRNA
cytokine
chemokine
description Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-02-11T12:42:41Z
dc.date.available.fl_str_mv 2022-02-11T12:42:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Marina Lima Silva et al. The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia. Front Cell Infect Microbiol., v. 11, 631333, 2021. doi: 10.3389/fcimb.2021.631333
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51152
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
identifier_str_mv SANTOS, Marina Lima Silva et al. The Interface Between Inflammatory Mediators and MicroRNAs in Plasmodium vivax Severe Thrombocytopenia. Front Cell Infect Microbiol., v. 11, 631333, 2021. doi: 10.3389/fcimb.2021.631333
2235-2988
url https://www.arca.fiocruz.br/handle/icict/51152
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media SA
publisher.none.fl_str_mv Frontiers Media SA
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51152/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51152/2/The%20Interface%20Between%20Inflammatory%20Mediators.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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