RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae)

Detalhes bibliográficos
Autor(a) principal: Finamore-Araujo, Paula
Data de Publicação: 2022
Outros Autores: Fonseca, Gabriel Lucio Silva da, Vieira, Cecília Stahl, Castro, Daniele Pereira de, Moreira, Otacilio Cruz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54878
Resumo: This study was financed in part by the Coordination for the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES) - Finance Code 001 (P.F.A). This work also received financial support from Inova Fiocruz (VPPCB-007-FIO-18-2-72), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Rio de Janeiro (FAPERJ). O.C.M. is a researcher fellow of CNPq1D (311539/2020-3) and FAPERJ (JCNE, E-26/203.031/2018). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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spelling Finamore-Araujo, PaulaFonseca, Gabriel Lucio Silva daVieira, Cecília StahlCastro, Daniele Pereira deMoreira, Otacilio Cruz2022-09-27T13:12:32Z2022-09-27T13:12:32Z2022FINAMORE-ARAUJO, Paula etal. RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae). PLoS Negl. Trop. Dis. v. 16, n 7. 16 p. July 2022.1935-2727https://www.arca.fiocruz.br/handle/icict/5487810.1371/journal.pntd.0010535This study was financed in part by the Coordination for the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES) - Finance Code 001 (P.F.A). This work also received financial support from Inova Fiocruz (VPPCB-007-FIO-18-2-72), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Rio de Janeiro (FAPERJ). O.C.M. is a researcher fellow of CNPq1D (311539/2020-3) and FAPERJ (JCNE, E-26/203.031/2018). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Niterói, RJ, Brasil.Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.A recurring question concerning Trypanosoma cruzi DNA detection/quantification is related to the fact that DNA amplification, by itself, does not differentiate between viable or dead parasites. On the other hand, RNA can be considered a potential molecular marker of patho gens viability. Herein, we developed a quantitative real-time PCR with reverse Transcription (RT-qPCR) to quantify viable T. cruzi in artificially infected Rhodnius prolixus whilst evaluat ing differences between DNA and mRNA quantification along the insect midgut during 5, 9, 15 and 29 days after feeding. The RT-qPCR presented an improved performance with line arities ranging from 107 to 102 parasites equivalents and 3 to 0.0032 intestine unit equiva lents, and efficiencies of 100.3% and 102.8% for both T. cruzi and triatomine targets, respectively. Comparing both RT-qPCR and qPCR, we confirmed that RNA is faster degraded, no longer being detected at day 1 after parasite lysis, while DNA detection was stable, with no decrease in parasite load over the days, even after parasite lysis. We also observed statistical differences between the quantification of the parasite load by DNA and by RNA on day 15 after feeding of experimentally infected R. prolixus. When assessing dif ferent portions of the digestive tract, by RT-qPCR, we could detect a statistically significant reduction in the parasite amount in the anterior midgut. Oppositely, there was a statistically significant increase of the parasite load in the hindgut. In conclusion, for this study parasite’s viability in R. prolixus digestive tract were assessed targeting T. cruzi mRNA. In addition, dif ferences between DNA and RNA detection observed herein, raise the possibility that RNA is a potential molecular viability marker, which could contribute to understanding the dynamics of the parasite infection in invertebrate hosts.engPublic Library of ScienceRNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTrypanosoma cruziRhodnius prolixusTriatominaeReal-Time PCRinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.en_US.fl_str_mv RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae)
title RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae)
spellingShingle RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae)
Finamore-Araujo, Paula
Trypanosoma cruzi
Rhodnius prolixus
Triatominae
Real-Time PCR
title_short RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae)
title_full RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae)
title_fullStr RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae)
title_full_unstemmed RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae)
title_sort RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae)
author Finamore-Araujo, Paula
author_facet Finamore-Araujo, Paula
Fonseca, Gabriel Lucio Silva da
Vieira, Cecília Stahl
Castro, Daniele Pereira de
Moreira, Otacilio Cruz
author_role author
author2 Fonseca, Gabriel Lucio Silva da
Vieira, Cecília Stahl
Castro, Daniele Pereira de
Moreira, Otacilio Cruz
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Finamore-Araujo, Paula
Fonseca, Gabriel Lucio Silva da
Vieira, Cecília Stahl
Castro, Daniele Pereira de
Moreira, Otacilio Cruz
dc.subject.en.en_US.fl_str_mv Trypanosoma cruzi
Rhodnius prolixus
Triatominae
Real-Time PCR
topic Trypanosoma cruzi
Rhodnius prolixus
Triatominae
Real-Time PCR
description This study was financed in part by the Coordination for the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES) - Finance Code 001 (P.F.A). This work also received financial support from Inova Fiocruz (VPPCB-007-FIO-18-2-72), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Rio de Janeiro (FAPERJ). O.C.M. is a researcher fellow of CNPq1D (311539/2020-3) and FAPERJ (JCNE, E-26/203.031/2018). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-09-27T13:12:32Z
dc.date.available.fl_str_mv 2022-09-27T13:12:32Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FINAMORE-ARAUJO, Paula etal. RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae). PLoS Negl. Trop. Dis. v. 16, n 7. 16 p. July 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54878
dc.identifier.issn.en_US.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0010535
identifier_str_mv FINAMORE-ARAUJO, Paula etal. RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae). PLoS Negl. Trop. Dis. v. 16, n 7. 16 p. July 2022.
1935-2727
10.1371/journal.pntd.0010535
url https://www.arca.fiocruz.br/handle/icict/54878
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/54878/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/54878/2/OtacilioMoreira_CeciliaVieira-etal_IOC_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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