Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice.

Detalhes bibliográficos
Autor(a) principal: Ferrazoli, Enéas Galdini
Data de Publicação: 2013
Outros Autores: Blanco, Miriam Marcela, Bittencourt, Simone Ribeiro, Bachi, André Luis Lacerda, Bahia, Luciana Mendes Bahia, Soares, Milena Botelho Pereira, Santos, Ricardo Ribeiro dos, Mello, Luiz Eugênio, Longo, Beatriz Monteiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8568
Resumo: Federal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, Brasil
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spelling Ferrazoli, Enéas GaldiniBlanco, Miriam MarcelaBittencourt, Simone RibeiroBachi, André Luis LacerdaBahia, Luciana Mendes BahiaSoares, Milena Botelho PereiraSantos, Ricardo Ribeiro dosMello, Luiz EugênioLongo, Beatriz Monteiro2014-10-09T17:27:50Z2014-10-09T17:27:50Z2013FERRAZOLI, E. G. et al. Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice. BMC Neuroscience, v. 14, p. 97, 2013.1471-2202https://www.arca.fiocruz.br/handle/icict/856810.1186/1471-2202-14-97engBioMed CentralAnticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, BrasilFederal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, BrasilFederal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, BrasilFederal University of São Paulo. UNIFESP. Departamento de Micro-Imuno-Parasitologia. São Paulo, BrazilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFederal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, BrasilFederal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, BrasilBACKGROUND: Bone marrow is an accessible source of progenitor cells, which have been investigated as treatment for neurological diseases in a number of clinical trials. Here we evaluated the potential benefit of bone marrow cells in protecting against convulsive seizures induced by maximum electroconvulsive shock (MES), a widely used model for screening of anti-epileptic drugs. Behavioral and inflammatory responses were measured after MES induction in order to verify the effects promoted by transplantation of bone marrow cells. To assess the anticonvulsant effects of bone marrow cell transplantation, we measured the frequency and duration of tonic seizure, the mortality rate, the microglial expression and the blood levels of cytokine IL-1, IL-6, IL-10 and TNF-α after MES induction. We hypothesized that these behavioral and inflammatory responses to a strong stimulus such as a convulsive seizure could be modified by the transplantation of bone marrow cells. RESULTS: Bone marrow transplanted cells altered the convulsive threshold and showed anticonvulsant effect by protecting from tonic seizures. Bone marrow cells modified the microglial expression in the analyzed brain areas, increased the IL-10 and attenuate IL-6 levels. CONCLUSIONS: Bone marrow cells exert protective effects by blocking the course of electroconvulsive seizures. Additionally, electroconvulsive seizures induced acute inflammatory responses by altering the pattern of microglia expression, as well as in IL-6 and IL-10 levels. Our findings also indicated that the anticonvulsant effects of these cells can be tested with the MES model following the same paradigm used for drug testing in pharmacological screening. Studies on the inflammatory reaction in response to acute seizures in the presence of transplanted bone marrow cells might open a wide range of discussions on the mechanisms relevant to the pathophysiology of epilepsies.Bone marrowCell transplantationElectroconvulsive shockTonic seizureMicrogliaHippocampusTransplante de Medula Óssea/métodosEncéfalo/citologiaMicroglia/citologiaConvulsões/terapiaAnimaisCitocinas/sangueEletrochoque/efeitos adversosImuno-HistoquímicaCamundongosCamundongos Endogâmicos C57BLinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8568/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALFerrazoli E G Anticonvulsant activity....pdfFerrazoli E G Anticonvulsant activity....pdfapplication/pdf583379https://www.arca.fiocruz.br/bitstream/icict/8568/2/Ferrazoli%20E%20G%20Anticonvulsant%20activity....pdfcc62d3b5cefbf1c6f55123a80ff5b5c0MD52TEXTFerrazoli E G Anticonvulsant activity....pdf.txtFerrazoli E G Anticonvulsant activity....pdf.txtExtracted texttext/plain40421https://www.arca.fiocruz.br/bitstream/icict/8568/3/Ferrazoli%20E%20G%20Anticonvulsant%20activity....pdf.txt77dc14542dc78ca6936be90d8208befbMD53icict/85682023-03-15 14:33:07.896oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:07Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice.
title Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice.
spellingShingle Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice.
Ferrazoli, Enéas Galdini
Bone marrow
Cell transplantation
Electroconvulsive shock
Tonic seizure
Microglia
Hippocampus
Transplante de Medula Óssea/métodos
Encéfalo/citologia
Microglia/citologia
Convulsões/terapia
Animais
Citocinas/sangue
Eletrochoque/efeitos adversos
Imuno-Histoquímica
Camundongos
Camundongos Endogâmicos C57BL
title_short Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice.
title_full Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice.
title_fullStr Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice.
title_full_unstemmed Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice.
title_sort Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice.
author Ferrazoli, Enéas Galdini
author_facet Ferrazoli, Enéas Galdini
Blanco, Miriam Marcela
Bittencourt, Simone Ribeiro
Bachi, André Luis Lacerda
Bahia, Luciana Mendes Bahia
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Mello, Luiz Eugênio
Longo, Beatriz Monteiro
author_role author
author2 Blanco, Miriam Marcela
Bittencourt, Simone Ribeiro
Bachi, André Luis Lacerda
Bahia, Luciana Mendes Bahia
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Mello, Luiz Eugênio
Longo, Beatriz Monteiro
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferrazoli, Enéas Galdini
Blanco, Miriam Marcela
Bittencourt, Simone Ribeiro
Bachi, André Luis Lacerda
Bahia, Luciana Mendes Bahia
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Mello, Luiz Eugênio
Longo, Beatriz Monteiro
dc.subject.en.pt_BR.fl_str_mv Bone marrow
Cell transplantation
Electroconvulsive shock
Tonic seizure
Microglia
Hippocampus
topic Bone marrow
Cell transplantation
Electroconvulsive shock
Tonic seizure
Microglia
Hippocampus
Transplante de Medula Óssea/métodos
Encéfalo/citologia
Microglia/citologia
Convulsões/terapia
Animais
Citocinas/sangue
Eletrochoque/efeitos adversos
Imuno-Histoquímica
Camundongos
Camundongos Endogâmicos C57BL
dc.subject.decs.pt_BR.fl_str_mv Transplante de Medula Óssea/métodos
Encéfalo/citologia
Microglia/citologia
Convulsões/terapia
Animais
Citocinas/sangue
Eletrochoque/efeitos adversos
Imuno-Histoquímica
Camundongos
Camundongos Endogâmicos C57BL
description Federal University of São Paulo. UNIFESP. Laboratório de Neurofisiologia. Departamento de Fisiologia. São Paulo, SP, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-10-09T17:27:50Z
dc.date.available.fl_str_mv 2014-10-09T17:27:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERRAZOLI, E. G. et al. Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice. BMC Neuroscience, v. 14, p. 97, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8568
dc.identifier.issn.none.fl_str_mv 1471-2202
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2202-14-97
identifier_str_mv FERRAZOLI, E. G. et al. Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice. BMC Neuroscience, v. 14, p. 97, 2013.
1471-2202
10.1186/1471-2202-14-97
url https://www.arca.fiocruz.br/handle/icict/8568
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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