Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4168 |
Resumo: | We investigated long-term environmental influences on morphology of microglia from the outer and middle thirds of molecular layer of the dentate gyrus (MolDG), and on microglia from dorsal and ventral dentate gyrus molecular layer. We also estimated the total number of MolDG microglia using stereology. For this purpose, microglia of the molecular layer of the dentate gyrus of 20-month-old female Swiss albino mice, housed from 21st postnatal day onwards, in the impoverished environment of the standard laboratory cages (SEA), or in a cage with an enriched environment (EEA), were reconstructed microscopically in three dimensions and compared with each other and with microglia of 6-month-old female Swiss albino mice, also housed from weaning onwards in an enriched cage (EEY). All mice had their brains sectioned and processed for immunolabeling for IBA-1, a selective microglia marker. Random and systematic microglia samples were reconstructed in three dimensions and classified morphologically using hierarchical cluster analysis, followed by discriminant function analysis. SEA and EEY showed two morphological phenotypes of microglia in both the outer and middle thirds of MolDG. EEA mice showed such a reduction in the morphological diversity of microglia that essentially a single morphotype was found. EEA mouse microglia showed an intermediate morphological complexity between types I and II SE microglia. We suggest that type I and type II microglia in SE mice may have different physiological roles and that long-term EE may be associated with adaptive responses of microglial phenotypes to somatomotor and cognitive stimuli. |
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Oliveira, Thaís Cristina Galdino deCarvalho-Paulo, DarioLima, Camila Mendes deOliveira, Roseane Borner deSantos Filho, CarlosDiniz, Daniel GuerreiroTorres Neto, João BentoDiniz, Cristovam Wanderley Picanço2020-09-01T16:53:59Z2020-09-01T16:53:59Z2020OLIVEIRA, Thaís Cristina Galdino de et al. Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus. European Journal of Neuroscience, v. xx, n. xx, p. xx, 2020.1460-9568https://patua.iec.gov.br/handle/iec/416810.1111/ejn.14920We investigated long-term environmental influences on morphology of microglia from the outer and middle thirds of molecular layer of the dentate gyrus (MolDG), and on microglia from dorsal and ventral dentate gyrus molecular layer. We also estimated the total number of MolDG microglia using stereology. For this purpose, microglia of the molecular layer of the dentate gyrus of 20-month-old female Swiss albino mice, housed from 21st postnatal day onwards, in the impoverished environment of the standard laboratory cages (SEA), or in a cage with an enriched environment (EEA), were reconstructed microscopically in three dimensions and compared with each other and with microglia of 6-month-old female Swiss albino mice, also housed from weaning onwards in an enriched cage (EEY). All mice had their brains sectioned and processed for immunolabeling for IBA-1, a selective microglia marker. Random and systematic microglia samples were reconstructed in three dimensions and classified morphologically using hierarchical cluster analysis, followed by discriminant function analysis. SEA and EEY showed two morphological phenotypes of microglia in both the outer and middle thirds of MolDG. EEA mice showed such a reduction in the morphological diversity of microglia that essentially a single morphotype was found. EEA mouse microglia showed an intermediate morphological complexity between types I and II SE microglia. We suggest that type I and type II microglia in SE mice may have different physiological roles and that long-term EE may be associated with adaptive responses of microglial phenotypes to somatomotor and cognitive stimuli.Fundação de Amparo à Pesquisa do Pará—FADESP; Pró‐Reitoria de Pesquisa e Pós‐Graduação da Universidade Federal do Pará—PROPESP Edital. Grant Number: 2019‐PIAPA; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES—Pró‐Amazônia. Grant Number: 3311/2013; Brazilian Research Council—CNPq. Grant Numbers: 307749/2004‐5, 471077/2007‐0; Fundação Amazônia de Amparo a Estudos e Pesquisas do Pará—FAPESPA, ICAAF. Grant Number: 039/2017; PROCAD‐AMAZÔNIA. Grant Number: 88881.370785/2019‐01Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.engFederation of European Neuroscience SocietiesLong‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCérebro / anatomia & histologiaGiro DenteadoMicroglia / citologiaCamundongosinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALLong‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus.pdfLong‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/16b11003-4d44-4199-a9c9-0874f3df5ca7/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/b1e29a37-a8b9-4801-b66b-24512203cc71/download11832eea31b16df8613079d742d61793MD52TEXTLong‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus.pdf.txtLong‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/fc62b273-33c3-4e64-8ea8-7ebc7f2c14e9/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55THUMBNAILLong‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus.pdf.jpgLong‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/0a32c283-bc3a-414f-8caf-7b64ea14358d/download71859d578212107f7f8c49a4ce09d9eeMD56iec/41682022-10-20 22:37:56.07oai:patua.iec.gov.br:iec/4168https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:37:56Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus |
title |
Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus |
spellingShingle |
Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus Oliveira, Thaís Cristina Galdino de Cérebro / anatomia & histologia Giro Denteado Microglia / citologia Camundongos |
title_short |
Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus |
title_full |
Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus |
title_fullStr |
Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus |
title_full_unstemmed |
Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus |
title_sort |
Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus |
author |
Oliveira, Thaís Cristina Galdino de |
author_facet |
Oliveira, Thaís Cristina Galdino de Carvalho-Paulo, Dario Lima, Camila Mendes de Oliveira, Roseane Borner de Santos Filho, Carlos Diniz, Daniel Guerreiro Torres Neto, João Bento Diniz, Cristovam Wanderley Picanço |
author_role |
author |
author2 |
Carvalho-Paulo, Dario Lima, Camila Mendes de Oliveira, Roseane Borner de Santos Filho, Carlos Diniz, Daniel Guerreiro Torres Neto, João Bento Diniz, Cristovam Wanderley Picanço |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Thaís Cristina Galdino de Carvalho-Paulo, Dario Lima, Camila Mendes de Oliveira, Roseane Borner de Santos Filho, Carlos Diniz, Daniel Guerreiro Torres Neto, João Bento Diniz, Cristovam Wanderley Picanço |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Cérebro / anatomia & histologia Giro Denteado Microglia / citologia Camundongos |
topic |
Cérebro / anatomia & histologia Giro Denteado Microglia / citologia Camundongos |
description |
We investigated long-term environmental influences on morphology of microglia from the outer and middle thirds of molecular layer of the dentate gyrus (MolDG), and on microglia from dorsal and ventral dentate gyrus molecular layer. We also estimated the total number of MolDG microglia using stereology. For this purpose, microglia of the molecular layer of the dentate gyrus of 20-month-old female Swiss albino mice, housed from 21st postnatal day onwards, in the impoverished environment of the standard laboratory cages (SEA), or in a cage with an enriched environment (EEA), were reconstructed microscopically in three dimensions and compared with each other and with microglia of 6-month-old female Swiss albino mice, also housed from weaning onwards in an enriched cage (EEY). All mice had their brains sectioned and processed for immunolabeling for IBA-1, a selective microglia marker. Random and systematic microglia samples were reconstructed in three dimensions and classified morphologically using hierarchical cluster analysis, followed by discriminant function analysis. SEA and EEY showed two morphological phenotypes of microglia in both the outer and middle thirds of MolDG. EEA mice showed such a reduction in the morphological diversity of microglia that essentially a single morphotype was found. EEA mouse microglia showed an intermediate morphological complexity between types I and II SE microglia. We suggest that type I and type II microglia in SE mice may have different physiological roles and that long-term EE may be associated with adaptive responses of microglial phenotypes to somatomotor and cognitive stimuli. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-09-01T16:53:59Z |
dc.date.available.fl_str_mv |
2020-09-01T16:53:59Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Thaís Cristina Galdino de et al. Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus. European Journal of Neuroscience, v. xx, n. xx, p. xx, 2020. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4168 |
dc.identifier.issn.-.fl_str_mv |
1460-9568 |
dc.identifier.doi.-.fl_str_mv |
10.1111/ejn.14920 |
identifier_str_mv |
OLIVEIRA, Thaís Cristina Galdino de et al. Long‐term environmental enrichment reduces microglia morphological diversity of the molecular layer of dentate gyrus. European Journal of Neuroscience, v. xx, n. xx, p. xx, 2020. 1460-9568 10.1111/ejn.14920 |
url |
https://patua.iec.gov.br/handle/iec/4168 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
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embargoedAccess |
dc.publisher.none.fl_str_mv |
Federation of European Neuroscience Societies |
publisher.none.fl_str_mv |
Federation of European Neuroscience Societies |
dc.source.none.fl_str_mv |
reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
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Instituto Evandro Chagas (IEC) |
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IEC |
institution |
IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
collection |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
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