Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8942 |
Resumo: | 2015-07-31 |
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Soares, Emanoella L.Shah, MohibullahSoares, Arlete A.Costa, José H.Carvalho, Paulo CostaDomont, Gilberto B.Nogueira, Fábio Cesar SousaCampos, Francisco A. P.2014-11-24T12:13:37Z2015-08-01T07:30:05Z2014SOARES, Emanoella L. et al. Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds. Journal of Proteome Research, v. 13, n. 8, p. 3562-3570, 2014.1535-3893https://www.arca.fiocruz.br/handle/icict/894210.1021/pr5004505engJournal of Proteome ResearchProteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seedsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2015-07-31Federal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.Federal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.Federal University of Ceara. Department of Biology. Campus do Pici, Fortaleza, CE, Brazil.Federal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Federal University of Rio de Janeiro. Proteomic Unit, Institute of Chemistry. Rio de Janeiro, RJ, Brazil.Federal University of Rio de Janeiro. Proteomic Unit, Institute of Chemistry. Rio de Janeiro, RJ, Brazil.Federal University of Ceara. Department of Biochemistry and Molecular Biology. Fortaleza, CE, Brazil.In this study, we performed a systematic proteomic analysis of the inner integument from developing seeds of Jatropha curcas and further explored the protein machinery responsible for generating the carbon and nitrogen sources to feed the growing embryo and endosperm. The inner integument of developing seeds was dissected into two sections called distal and proximal, and proteins were extracted from these sections and from the whole integument and analyzed using an EASY-nanoLC system coupled to an ESILTQ-Orbitrap Velos mass spectrometer. We identified 1526, 1192, and 1062 proteins from the proximal, distal, and whole inner integuments, respectively. The identifications include those of peptidases and other hydrolytic enzymes that play a key role in developmental programmed cell death and proteins associated with the cell-wall architecture and modification. Because many of these proteins are differentially expressed within the integument cell layers, these findings suggest that the cells mobilize an array of hydrolases to produce carbon and nitrogen sources from proteins, carbohydrates, and lipids available within the cells. Not least, the identification of several classes of seed storage proteins in the inner integument provides additional evidence of the role of the seed coat as a transient source of reserves for the growing embryo and endosperm.Label-free quantitative proteomicsProgrammed cell deathJatropha curcasOilseedSeed coatinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALartigo1.pdfartigo1.pdfapplication/pdf5207876https://www.arca.fiocruz.br/bitstream/icict/8942/2/artigo1.pdf11e8e1f47f0046ef1b5b476cde62a038MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8942/3/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD53TEXTartigo1.pdf.txtartigo1.pdf.txtExtracted texttext/plain51931https://www.arca.fiocruz.br/bitstream/icict/8942/4/artigo1.pdf.txt3baa29afcc0d222f1eb930deda44a601MD54icict/89422021-03-24 16:32:18.184oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds |
title |
Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds |
spellingShingle |
Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds Soares, Emanoella L. Label-free quantitative proteomics Programmed cell death Jatropha curcas Oilseed Seed coat |
title_short |
Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds |
title_full |
Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds |
title_fullStr |
Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds |
title_full_unstemmed |
Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds |
title_sort |
Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds |
author |
Soares, Emanoella L. |
author_facet |
Soares, Emanoella L. Shah, Mohibullah Soares, Arlete A. Costa, José H. Carvalho, Paulo Costa Domont, Gilberto B. Nogueira, Fábio Cesar Sousa Campos, Francisco A. P. |
author_role |
author |
author2 |
Shah, Mohibullah Soares, Arlete A. Costa, José H. Carvalho, Paulo Costa Domont, Gilberto B. Nogueira, Fábio Cesar Sousa Campos, Francisco A. P. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Soares, Emanoella L. Shah, Mohibullah Soares, Arlete A. Costa, José H. Carvalho, Paulo Costa Domont, Gilberto B. Nogueira, Fábio Cesar Sousa Campos, Francisco A. P. |
dc.subject.en.pt_BR.fl_str_mv |
Label-free quantitative proteomics Programmed cell death Jatropha curcas Oilseed Seed coat |
topic |
Label-free quantitative proteomics Programmed cell death Jatropha curcas Oilseed Seed coat |
description |
2015-07-31 |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-11-24T12:13:37Z |
dc.date.issued.fl_str_mv |
2014 |
dc.date.available.fl_str_mv |
2015-08-01T07:30:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOARES, Emanoella L. et al. Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds. Journal of Proteome Research, v. 13, n. 8, p. 3562-3570, 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/8942 |
dc.identifier.issn.none.fl_str_mv |
1535-3893 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1021/pr5004505 |
identifier_str_mv |
SOARES, Emanoella L. et al. Proteome Analysis of the Inner Integument from Developing Jatropha curcas L. Seeds. Journal of Proteome Research, v. 13, n. 8, p. 3562-3570, 2014. 1535-3893 10.1021/pr5004505 |
url |
https://www.arca.fiocruz.br/handle/icict/8942 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Journal of Proteome Research |
publisher.none.fl_str_mv |
Journal of Proteome Research |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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