Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility

Detalhes bibliográficos
Autor(a) principal: Smaniotto, Salete
Data de Publicação: 2013
Outros Autores: Schneider, Elke, Goudin, Nicolas, Bricard-Rignault, Rachel, Machavoine, François, Dardenne, Mireille, Dy, Michel, Savino, Wilson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10679
Resumo: Universidade Federal de Alagoas. Instituto de Ciências Biológicas e da Saúde. Laboratório de Biologia Celular.. Maceió, AL, Brasil.
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spelling Smaniotto, SaleteSchneider, ElkeGoudin, NicolasBricard-Rignault, RachelMachavoine, FrançoisDardenne, MireilleDy, MichelSavino, Wilson2015-06-08T14:01:47Z2015-06-08T14:01:47Z2013SMANIOTTO, Salete et al. Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility. Plos One, v.8, n.9, 8p, sept. 2013.https://www.arca.fiocruz.br/handle/icict/1067910.1371/journal.pone.0070292engPlos OneMouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motilityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Alagoas. Instituto de Ciências Biológicas e da Saúde. Laboratório de Biologia Celular.. Maceió, AL, Brasil.Université Paris Descartes. Hôpital Necker. CNRS UMR-8147. Paris, France.Université Paris Descartes. Hôpital Necker. Cell Imaging Platform. Paris, France.Université Paris Descartes. Hôpital Necker. CNRS UMR-8147. Paris, France.Université Paris Descartes. Hôpital Necker. CNRS UMR-8147. Paris, France.Université Paris Descartes. Hôpital Necker. CNRS UMR-8147. Paris, France.Université Paris Descartes. Hôpital Necker. CNRS UMR-8147. Paris, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Basophils co-express FceRIa and CD49b, the a-2 chain of integrin-type receptor VLA-2 (a2b1), which recognizes type-1 collagen as a major natural ligand. The physiological relevance of this integrin for interactions with extracellular bone marrow matrix remains unknown. Herein, we examined the expression of several receptors of this family by bone marrowderived basophils sorted either ex-vivo or after culture with IL-3. Having established that both populations display CD49d, CD49e and CD49f (a-4, a-5 and a-6 integrins subunits, respectively), we addressed receptor functions by measuring migration, adhesion, proliferation and survival after interacting with matched natural ligands. Type I collagen, laminin and fibronectin promoted basophil migration/adhesion, the former being the most effective. None of these ligands affected basophil viability and expansion. Interactions between basophils and extracellular matrix are likely to play a role in situ, as supported by confocal 3D cell imaging of femoral bone marrow sections, which revealed basophils exclusively in type-1 collagen-enriched niches that contained likewise laminin and fibronectin. This is the first evidence for a structure/function relationship between basophils and extracellular matrix proteins inside the mouse bone marrow.Mouse BasophilsBone MarrowExtracellular MatrixMedula ÒsseaBasófilosMatriz Extracelularinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10679/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALwilson_sovino2etal_IOC_2014.pdfapplication/pdf3263108https://www.arca.fiocruz.br/bitstream/icict/10679/2/wilson_sovino2etal_IOC_2014.pdf9132ef912d83ec3454d9e1b5b492795bMD52TEXTwilson_sovino2etal_IOC_2014.pdf.txtwilson_sovino2etal_IOC_2014.pdf.txtExtracted texttext/plain34057https://www.arca.fiocruz.br/bitstream/icict/10679/3/wilson_sovino2etal_IOC_2014.pdf.txt44ae51cbdfd4e2a4a168618987bc7bf4MD53icict/106792022-06-24 13:11:00.136oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:11Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility
title Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility
spellingShingle Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility
Smaniotto, Salete
Mouse Basophils
Bone Marrow
Extracellular Matrix
Medula Òssea
Basófilos
Matriz Extracelular
title_short Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility
title_full Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility
title_fullStr Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility
title_full_unstemmed Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility
title_sort Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility
author Smaniotto, Salete
author_facet Smaniotto, Salete
Schneider, Elke
Goudin, Nicolas
Bricard-Rignault, Rachel
Machavoine, François
Dardenne, Mireille
Dy, Michel
Savino, Wilson
author_role author
author2 Schneider, Elke
Goudin, Nicolas
Bricard-Rignault, Rachel
Machavoine, François
Dardenne, Mireille
Dy, Michel
Savino, Wilson
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Smaniotto, Salete
Schneider, Elke
Goudin, Nicolas
Bricard-Rignault, Rachel
Machavoine, François
Dardenne, Mireille
Dy, Michel
Savino, Wilson
dc.subject.en.pt_BR.fl_str_mv Mouse Basophils
Bone Marrow
Extracellular Matrix
topic Mouse Basophils
Bone Marrow
Extracellular Matrix
Medula Òssea
Basófilos
Matriz Extracelular
dc.subject.decs.pt_BR.fl_str_mv Medula Òssea
Basófilos
Matriz Extracelular
description Universidade Federal de Alagoas. Instituto de Ciências Biológicas e da Saúde. Laboratório de Biologia Celular.. Maceió, AL, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-06-08T14:01:47Z
dc.date.available.fl_str_mv 2015-06-08T14:01:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SMANIOTTO, Salete et al. Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility. Plos One, v.8, n.9, 8p, sept. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10679
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pone.0070292
identifier_str_mv SMANIOTTO, Salete et al. Mouse Basophils Reside in Extracellular Matrix-Enriched Bone Marrow Niches Which Control Their Motility. Plos One, v.8, n.9, 8p, sept. 2013.
10.1371/journal.pone.0070292
url https://www.arca.fiocruz.br/handle/icict/10679
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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