Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7823 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil |
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Parreiras, Patrícia MartinsAndrade, Giovanna IvoNascimento, Telma de Figueiredo doOelemann, Maraníbia CardosoGomes, Harrison MagdinierAlencar, Andrea Padilha deAssis, Ronnie Antunes deMota, Pedro Moacyr Pinto CoelhoPereira, Márcia Aparecida da SilvaLobato, Francisco Carlos FariaLage, Andrey PereiraSuffys, Philip Noel2014-06-17T12:56:00Z2014-06-17T12:56:00Z2012PARREIRAS, Patrícia Martins et al. Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil. Memórias do Instituto Oswaldo Cruz. 2012; 107(1): 64-730074-0276https://www.arca.fiocruz.br/handle/icict/782310.1590/S0074-02762012000100009engFundação Oswaldo CruzSpoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, BrasilLaboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo, MG, BrasilLaboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo, MG, BrasilLaboratório Nacional Agropecuário de Minas Gerais. Pedro Leopoldo, MG, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacterioses e Pesquisa. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, BrasilWe performed spoligotyping and 12-mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTRs) typing to characterise Mycobacterium bovis isolates collected from tissue samples of bovines with lesions suggestive for tuberculosis during slaughter inspection procedures in abattoirs in Brazil. High-quality genotypes were obtained with both procedures for 61 isolates that were obtained from 185 bovine tissue samples and all of these isolates were identified as M. bovis by conventional identification procedures. On the basis of the spoligotyping, 53 isolates were grouped into nine clusters and the remaining eight isolates were unique types, resulting in 17 spoligotypes. The majority of the Brazilian M. bovis isolates displayed spoligotype patterns that have been previously observed in strains isolated from cattle in other countries. MIRU-VNTR typing produced 16 distinct genotypes, with 53 isolates forming eight of the groups, and individual isolates with unique VNTR profiles forming the remaining eight groups. The allelic diversity of each VNTR locus was calculated and only two of the 12-MIRU-VNTR loci presented scores with either a moderate (0.4, MIRU16) or high (0.6, MIRU26) discriminatory index (h). Both typing methods produced similar discriminatory indexes (spoligotyping h = 0.85; MIRU-VNTR h = 0.86) and the combination of the two methods increased the h value to 0.94, resulting in 29 distinct patterns. These results confirm that spoligotyping and VNTR analysis are valuable tools for studying the molecular epidemiology of M. bovis infections in Brazil.Mycobacterium bovisspoligotypingMIRU-VNTR typingbovine tuberculosismolecular epidemiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSpoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil.pdfSpoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil.pdfapplication/pdf1905023https://www.arca.fiocruz.br/bitstream/icict/7823/1/Spoligotyping%20and%20variable%20number%20tandem%20repeat%20analysis%20of%20Mycobacterium%20bovis%20isolates%20from%20cattle%20in%20Brazil.pdf5c523c0d7b75fb6454e3b495431275c4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7823/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTSpoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil.pdf.txtSpoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil.pdf.txtExtracted texttext/plain10https://www.arca.fiocruz.br/bitstream/icict/7823/5/Spoligotyping%20and%20variable%20number%20tandem%20repeat%20analysis%20of%20Mycobacterium%20bovis%20isolates%20from%20cattle%20in%20Brazil.pdf.txt2c6eb67c8897d916ae47524b1a844d3fMD55THUMBNAILSpoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil.pdf.jpgSpoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil.pdf.jpgGenerated Thumbnailimage/jpeg2159https://www.arca.fiocruz.br/bitstream/icict/7823/4/Spoligotyping%20and%20variable%20number%20tandem%20repeat%20analysis%20of%20Mycobacterium%20bovis%20isolates%20from%20cattle%20in%20Brazil.pdf.jpgb43b6b2495f0423f88d7999ee1d9bc24MD54icict/78232018-04-06 11:25:52.265oai:www.arca.fiocruz.br:icict/7823TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T14:25:52Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil |
| title |
Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil |
| spellingShingle |
Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil Parreiras, Patrícia Martins Mycobacterium bovis spoligotyping MIRU-VNTR typing bovine tuberculosis molecular epidemiology |
| title_short |
Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil |
| title_full |
Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil |
| title_fullStr |
Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil |
| title_full_unstemmed |
Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil |
| title_sort |
Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil |
| author |
Parreiras, Patrícia Martins |
| author_facet |
Parreiras, Patrícia Martins Andrade, Giovanna Ivo Nascimento, Telma de Figueiredo do Oelemann, Maraníbia Cardoso Gomes, Harrison Magdinier Alencar, Andrea Padilha de Assis, Ronnie Antunes de Mota, Pedro Moacyr Pinto Coelho Pereira, Márcia Aparecida da Silva Lobato, Francisco Carlos Faria Lage, Andrey Pereira Suffys, Philip Noel |
| author_role |
author |
| author2 |
Andrade, Giovanna Ivo Nascimento, Telma de Figueiredo do Oelemann, Maraníbia Cardoso Gomes, Harrison Magdinier Alencar, Andrea Padilha de Assis, Ronnie Antunes de Mota, Pedro Moacyr Pinto Coelho Pereira, Márcia Aparecida da Silva Lobato, Francisco Carlos Faria Lage, Andrey Pereira Suffys, Philip Noel |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Parreiras, Patrícia Martins Andrade, Giovanna Ivo Nascimento, Telma de Figueiredo do Oelemann, Maraníbia Cardoso Gomes, Harrison Magdinier Alencar, Andrea Padilha de Assis, Ronnie Antunes de Mota, Pedro Moacyr Pinto Coelho Pereira, Márcia Aparecida da Silva Lobato, Francisco Carlos Faria Lage, Andrey Pereira Suffys, Philip Noel |
| dc.subject.en.pt_BR.fl_str_mv |
Mycobacterium bovis spoligotyping MIRU-VNTR typing bovine tuberculosis molecular epidemiology |
| topic |
Mycobacterium bovis spoligotyping MIRU-VNTR typing bovine tuberculosis molecular epidemiology |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil |
| publishDate |
2012 |
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2012 |
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2014-06-17T12:56:00Z |
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2014-06-17T12:56:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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PARREIRAS, Patrícia Martins et al. Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil. Memórias do Instituto Oswaldo Cruz. 2012; 107(1): 64-73 |
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https://www.arca.fiocruz.br/handle/icict/7823 |
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0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762012000100009 |
| identifier_str_mv |
PARREIRAS, Patrícia Martins et al. Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil. Memórias do Instituto Oswaldo Cruz. 2012; 107(1): 64-73 0074-0276 10.1590/S0074-02762012000100009 |
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info:eu-repo/semantics/openAccess |
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Fundação Oswaldo Cruz |
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Fundação Oswaldo Cruz |
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