Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved

Detalhes bibliográficos
Autor(a) principal: Carvalho, Regina Pereira
Data de Publicação: 2013
Outros Autores: Aguiar, Carolina de Oliveira Mendes, Oliveira Neto, Manoel Paes de, Covas, Claudia de Jesus Fernandes, Santos, Álvaro Luiz Bertho dos, Cruz, Alda Maria da, Silva, Adriano Gomes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1371/journal.pone.0081529
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10645
Resumo: IOC/FIOCRUZ, PAPESIV/VPPDT/FIOCRUZ, FAPERJ
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spelling Carvalho, Regina PereiraAguiar, Carolina de Oliveira MendesOliveira Neto, Manoel Paes deCovas, Claudia de Jesus FernandesSantos, Álvaro Luiz Bertho dosCruz, Alda Maria daSilva, Adriano Gomes2015-06-03T17:25:46Z2015-06-03T17:25:46Z2013CARVALHO, Regina Pereira et al. Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved. Plos One, v. 8, n.11, p. 1-8, 2013.1932-6203https://www.arca.fiocruz.br/handle/icict/1064510.1371/journal.pone.00815291932-6203IOC/FIOCRUZ, PAPESIV/VPPDT/FIOCRUZ, FAPERJFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Leishmania (Viannia) braziliensis control and tissue damage relate to the effector immune response, which in turn affects clinical outcome. Leishmania reactive CD4+ and CD8+ T cells are expanded in long-term healed cutaneous leishmaniasis (hCL) patients but their functional characteristics remain to be determined. This study investigates antigen-specific recall in long-term healed CL caused by L. braziliensis infection. Healed CL subjects were grouped according to the time elapsed since the end of therapy: less than two years and two to five years. Activation phenotype (CD69+ or CD25+) and subpopulations of memory T cell phenotypes [central memory (TCM): CD45RO+CCR7+ or effector memory (Tem): CD45RO+ CCR7-] were quantified in ex vivo blood mononuclear cells and after Leishmania antigens stimuli. A reduction in the percentage of activated Leishmania-responder CD4+ and CD8+ T cells in hCL was associated with the time elapsed since clinical cure. Percentage of CD69+ in TCD4+ and TCD8+ cells were negatively correlated with IL-10 levels. Ex vivo analyses showed contracted Tem CD4+ and Tem CD8+compartments from hCL with long time elapsed since clinical cure, although renewal of these compartments was observed following in vitro exposure to leishmanial stimuli. Our results show that healed L. braziliensis infected patients exhibit a recall response to Leishmania antigens with evident expansion of effector memory T cells. Regulated leishmanial-specific response seems to emerge only about two years after initial contact with the parasite antigens.engPublic Library of ScienceLeishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preservedinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmaniasisCutaneous LeishmaniasisLeishmania braziliensisCD4 antigensCD8 antigensLeishmanioseLeishmaniose cutâneaLeishmania braziliensisAntígenos CD4Antígenos CD8info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/10645/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALLeishmania braziliensis-reactive T cells are down-regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of T effector memory compartments is preserved.pdfLeishmania braziliensis-reactive T cells are down-regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of T effector memory compartments is preserved.pdfapplication/pdf800318https://www.arca.fiocruz.br/bitstream/icict/10645/2/Leishmania%20braziliensis-reactive%20T%20cells%20are%20down-regulated%20in%20long-term%20cured%20cutaneous%20leishmaniasis%2c%20but%20the%20renewal%20capacity%20of%20T%20effector%20memory%20compartments%20is%20preserved.pdf0dbe5e9651824379147ece8453937f6eMD52TEXTLeishmania braziliensis-reactive T cells are down-regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of T effector memory compartments is preserved.pdf.txtLeishmania braziliensis-reactive T cells are down-regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of T effector memory compartments is preserved.pdf.txtExtracted texttext/plain43544https://www.arca.fiocruz.br/bitstream/icict/10645/3/Leishmania%20braziliensis-reactive%20T%20cells%20are%20down-regulated%20in%20long-term%20cured%20cutaneous%20leishmaniasis%2c%20but%20the%20renewal%20capacity%20of%20T%20effector%20memory%20compartments%20is%20preserved.pdf.txt6bc93a18a2ae601a37a73bc56dade33fMD53icict/106452019-09-10 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dc.title.pt_BR.fl_str_mv Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved
title Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved
spellingShingle Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved
Carvalho, Regina Pereira
Leishmaniasis
Cutaneous Leishmaniasis
Leishmania braziliensis
CD4 antigens
CD8 antigens
Leishmaniose
Leishmaniose cutânea
Leishmania braziliensis
Antígenos CD4
Antígenos CD8
title_short Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved
title_full Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved
title_fullStr Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved
title_full_unstemmed Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved
title_sort Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved
author Carvalho, Regina Pereira
author_facet Carvalho, Regina Pereira
Aguiar, Carolina de Oliveira Mendes
Oliveira Neto, Manoel Paes de
Covas, Claudia de Jesus Fernandes
Santos, Álvaro Luiz Bertho dos
Cruz, Alda Maria da
Silva, Adriano Gomes
author_role author
author2 Aguiar, Carolina de Oliveira Mendes
Oliveira Neto, Manoel Paes de
Covas, Claudia de Jesus Fernandes
Santos, Álvaro Luiz Bertho dos
Cruz, Alda Maria da
Silva, Adriano Gomes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Regina Pereira
Aguiar, Carolina de Oliveira Mendes
Oliveira Neto, Manoel Paes de
Covas, Claudia de Jesus Fernandes
Santos, Álvaro Luiz Bertho dos
Cruz, Alda Maria da
Silva, Adriano Gomes
dc.subject.en.pt_BR.fl_str_mv Leishmaniasis
Cutaneous Leishmaniasis
Leishmania braziliensis
CD4 antigens
CD8 antigens
topic Leishmaniasis
Cutaneous Leishmaniasis
Leishmania braziliensis
CD4 antigens
CD8 antigens
Leishmaniose
Leishmaniose cutânea
Leishmania braziliensis
Antígenos CD4
Antígenos CD8
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose
Leishmaniose cutânea
Leishmania braziliensis
Antígenos CD4
Antígenos CD8
description IOC/FIOCRUZ, PAPESIV/VPPDT/FIOCRUZ, FAPERJ
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-06-03T17:25:46Z
dc.date.available.fl_str_mv 2015-06-03T17:25:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Regina Pereira et al. Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved. Plos One, v. 8, n.11, p. 1-8, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10645
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0081529
dc.identifier.eissn.none.fl_str_mv 1932-6203
identifier_str_mv CARVALHO, Regina Pereira et al. Leishmania braziliensis-reactive T cells are down- regulated in long-term cured cutaneous leishmaniasis, but the renewal capacity of t effector memory compartments is preserved. Plos One, v. 8, n.11, p. 1-8, 2013.
1932-6203
10.1371/journal.pone.0081529
url https://www.arca.fiocruz.br/handle/icict/10645
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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