Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing

Detalhes bibliográficos
Autor(a) principal: Faria, Carolina Arruda de
Data de Publicação: 2021
Outros Autores: Silva Júnior, Wilson Araújo, Coelho, Karoline Brito Caetano Andrade, Bassi, Mirian, Colombari, Eduardo, Zanette, Dalila Lucíola, Ribeiro-Paes, João Tadeu
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50058
Resumo: Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Genética. Ribeirão Preto, SP, Brasil.
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spelling Faria, Carolina Arruda deSilva Júnior, Wilson AraújoCoelho, Karoline Brito Caetano AndradeBassi, MirianColombari, EduardoZanette, Dalila LucíolaRibeiro-Paes, João Tadeu2021-11-24T19:52:36Z2021-11-24T19:52:36Z2021FARIA, Carolina Arruda de et al. Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing. Pulmonary Pharmacology & Therapeutics. v. 70, n. 102075, p. 1–10, 2021.0009-2797https://www.arca.fiocruz.br/handle/icict/5005810.1016/j.pupt.2021.102075porElsevier. Academic PressCOPDTerapia CelularMSCSinvastatinaChronic Obstructive Pulmonary DiseaseCell- and Tissue-Based TherapyMesenchymal Stem CellsSimvastatinEnfermedad Pulmonar Obstructiva CrónicaTratamiento Basado en Trasplante de Células y TejidosCélulas Madre MesenquimatosasSimvastatinaBroncho-pneumopathie chronique obstructiveThérapie cellulaire et tissulaireCellules souches mésenchymateusesSimvastatineDoença Pulmonar Obstrutiva CrônicaTerapia Baseada em Transplante de Células e TecidosCélulas-Tronco MesenquimaisMesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular hominginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Genética. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Genética. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Cirurgia e Anatomia. Ribeirão Preto, SP, Brasil.Universidade Estadual Paulista. Faculdade de Odontologia. Departamento de Fisiologia e Patologia. Araraquara, SP, Brasil.Universidade Estadual Paulista. Faculdade de Odontologia. Departamento de Fisiologia e Patologia. Araraquara, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Universidade Estadual Paulista. Departamento de Biotecnologia. ASSIS, SP, Brasil.Chronic Obstructive Pulmonary Disease - COPD is characterized by the destruction of alveolar walls associated to a chronic inflammatory response of the airways. There is no clinical therapy for COPD. In this context, cell-based therapies represent a promising therapeutic approach for chronic lung disease. The goal of this work was to evaluate the effect of simvastatin on cell-based therapy in a mice emphysema model. Female FVB mice received intranasal instillation of elastase (three consecutive doses of 50 μL) in order to promote pulmonary emphysema. After 21 days of the first instillation, the animals were treated with Adipose-Derived Mesenchymal Stromal Cells (AD-MSC, 2.6 × 106 ) via retro-orbital infusion associated or not with simvastatin administrated daily via oral gavage (15 mg/kg/15d). Before and after these treatments, the histological and morphometrical analyses of the lung tissue, as so as lung function (whole body plethysmography) were evaluated. PAI-1 gene expression, an upregulated factor by ischemia that indicate a low survival of transplanted MSC, was also evaluated. The result regarding morphological and functional aspects of both lungs, presented no significant difference among the groups (AD-MSC or AD-MSC + Simvastatin). However, significant anatomical difference was observed in the right lung of the both groups of mice. The results shown a higher deposition of cells in the right lung, with might to be explained by anatomical differences (slightly higher right bronchi). Decreased levels of PAI-1 were observed in the simvastatin treated groups. The pulmonary ventilation was similar between the groups with only a tendency to a lower in the elastase treated animals due to a low respiratory frequency. In conclusion, the results suggest that both AD-MSC and simvastatin treatments could promote an improvement of morphological recovery of pulmonary emphysema, that it was more pronounced in the right lung.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/50058/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL1-s2.0-S1094553921000870-OK.pdf1-s2.0-S1094553921000870-OK.pdfapplication/pdf6270376https://www.arca.fiocruz.br/bitstream/icict/50058/2/1-s2.0-S1094553921000870-OK.pdf9d0e2f3308d3f649c2eb299b4b43d55dMD52icict/500582021-11-24 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dc.title.pt_BR.fl_str_mv Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing
title Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing
spellingShingle Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing
Faria, Carolina Arruda de
COPD
Terapia Celular
MSC
Sinvastatina
Chronic Obstructive Pulmonary Disease
Cell- and Tissue-Based Therapy
Mesenchymal Stem Cells
Simvastatin
Enfermedad Pulmonar Obstructiva Crónica
Tratamiento Basado en Trasplante de Células y Tejidos
Células Madre Mesenquimatosas
Simvastatina
Broncho-pneumopathie chronique obstructive
Thérapie cellulaire et tissulaire
Cellules souches mésenchymateuses
Simvastatine
Doença Pulmonar Obstrutiva Crônica
Terapia Baseada em Transplante de Células e Tecidos
Células-Tronco Mesenquimais
title_short Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing
title_full Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing
title_fullStr Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing
title_full_unstemmed Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing
title_sort Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing
author Faria, Carolina Arruda de
author_facet Faria, Carolina Arruda de
Silva Júnior, Wilson Araújo
Coelho, Karoline Brito Caetano Andrade
Bassi, Mirian
Colombari, Eduardo
Zanette, Dalila Lucíola
Ribeiro-Paes, João Tadeu
author_role author
author2 Silva Júnior, Wilson Araújo
Coelho, Karoline Brito Caetano Andrade
Bassi, Mirian
Colombari, Eduardo
Zanette, Dalila Lucíola
Ribeiro-Paes, João Tadeu
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Faria, Carolina Arruda de
Silva Júnior, Wilson Araújo
Coelho, Karoline Brito Caetano Andrade
Bassi, Mirian
Colombari, Eduardo
Zanette, Dalila Lucíola
Ribeiro-Paes, João Tadeu
dc.subject.other.pt_BR.fl_str_mv COPD
Terapia Celular
MSC
Sinvastatina
topic COPD
Terapia Celular
MSC
Sinvastatina
Chronic Obstructive Pulmonary Disease
Cell- and Tissue-Based Therapy
Mesenchymal Stem Cells
Simvastatin
Enfermedad Pulmonar Obstructiva Crónica
Tratamiento Basado en Trasplante de Células y Tejidos
Células Madre Mesenquimatosas
Simvastatina
Broncho-pneumopathie chronique obstructive
Thérapie cellulaire et tissulaire
Cellules souches mésenchymateuses
Simvastatine
Doença Pulmonar Obstrutiva Crônica
Terapia Baseada em Transplante de Células e Tecidos
Células-Tronco Mesenquimais
dc.subject.en.pt_BR.fl_str_mv Chronic Obstructive Pulmonary Disease
Cell- and Tissue-Based Therapy
Mesenchymal Stem Cells
Simvastatin
dc.subject.es.pt_BR.fl_str_mv Enfermedad Pulmonar Obstructiva Crónica
Tratamiento Basado en Trasplante de Células y Tejidos
Células Madre Mesenquimatosas
Simvastatina
dc.subject.fr.pt_BR.fl_str_mv Broncho-pneumopathie chronique obstructive
Thérapie cellulaire et tissulaire
Cellules souches mésenchymateuses
Simvastatine
dc.subject.decs.pt_BR.fl_str_mv Doença Pulmonar Obstrutiva Crônica
Terapia Baseada em Transplante de Células e Tecidos
Células-Tronco Mesenquimais
description Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Genética. Ribeirão Preto, SP, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-24T19:52:36Z
dc.date.available.fl_str_mv 2021-11-24T19:52:36Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIA, Carolina Arruda de et al. Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing. Pulmonary Pharmacology & Therapeutics. v. 70, n. 102075, p. 1–10, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50058
dc.identifier.issn.pt_BR.fl_str_mv 0009-2797
dc.identifier.doi.none.fl_str_mv 10.1016/j.pupt.2021.102075
identifier_str_mv FARIA, Carolina Arruda de et al. Mesenchymal stromal cells-based therapy in a murine model of elastase-induced emphysema: Simvastatin as a potential adjuvant in cellular homing. Pulmonary Pharmacology & Therapeutics. v. 70, n. 102075, p. 1–10, 2021.
0009-2797
10.1016/j.pupt.2021.102075
url https://www.arca.fiocruz.br/handle/icict/50058
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier. Academic Press
publisher.none.fl_str_mv Elsevier. Academic Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50058/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50058/2/1-s2.0-S1094553921000870-OK.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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