Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis.

Detalhes bibliográficos
Autor(a) principal: Oliveira, Sheilla Andrade de
Data de Publicação: 2012
Outros Autores: Souza, Bruno Solano de Freitas, Barreto, Elton Pereira Sá, Kaneto, Carla Martins, Almeida Neto, Hélio, Azevedo, Carine Machado, Guimarães, Elisalva Teixeira, Freitas, Luiz Antonio Rodrigues de, Santos, Ricardo Ribeiro dos, Soares, Milena Botelho Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8572
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhaes. Recife, PE, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Oliveira, Sheilla Andrade deSouza, Bruno Solano de FreitasBarreto, Elton Pereira SáKaneto, Carla MartinsAlmeida Neto, HélioAzevedo, Carine MachadoGuimarães, Elisalva TeixeiraFreitas, Luiz Antonio Rodrigues deSantos, Ricardo Ribeiro dosSoares, Milena Botelho Pereira2014-10-09T19:21:46Z2014-10-09T19:21:46Z2012OLIVEIRA, S. A. et al. Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis. Cytotherapy, v. 14, n. 3, p. 339-349, 2012.1477-2566https://www.arca.fiocruz.br/handle/icict/857210.3109/14653249.2011.637668engInforma HealthcareReduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhaes. Recife, PE, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilHospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilHospital São Rafael. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilBackground aims. Cirrhosis, end-stage liver disease, is caused by different mechanisms of injury, associated with persistent infl ammation. Galectin-3 is an important regulator of fi brosis that links chronic infl ammation to fi brogenesis. We investigated the role of bone marrow cell (BMC) transplantation in chronic infl ammation and hepatic fi brosis. Methods . Liver cirrhosis was induced by administration of carbon tetrachloride and ethanol to wild-type C57BL/6 or bone marrow chimeric mice. Bone marrow chimeras were generated by lethal irradiation and transplantation with BMC obtained from green fl uorescent protein (GFP )donors. Wild-type cirrhotic mice were transplanted with BMC without irradiation. Livers from chimeras and cirrhotic transplanted mice were obtained for evaluation of infl ammation, fi brosis and regulatory factors [galectin-3, matrix metallopeptidase (MMP)-9, tissue inhibitor of metalloproteinase (TIMP)-1 and transforming growth factor (TGF)- β ]. Results . The development of cirrhosis was associated with increased expression of galectin-3 by F4/80 cells and intense migration of BMC to the liver. Furthermore, when transplanted after the establishment of cirrhosis, BMC also migrated to the liver and localized within the fi brous septa. Two months after BMC therapy, cirrhotic mice had a signifi cant reduction in liver fi brosis and expression of type I collagen. We did not fi nd any difference in levels of TGF- β , TIMP-1 and MMP-9 between saline and BMC groups. However, the numbers of infl ammatory cells, phagocytes and galectin-3 cells were markedly lower in the livers of cirrhotic mice treated with BMC. Conclusions . Our results demonstrate an important role for BMC in the regulation of liver fi brosis and that transplantation of BMC can accelerate fi brosis regression through modulatory mechanismsCarbon tetrachlorideGalectin-3Hepatic cirrhosisMiceStem cellsTransplante de Medula Óssea/métodosGalectina 3/metabolismoCirrose Hepática Experimental/terapiaAnimaisCélulas da Medula Óssea/citologiaCélulas da Medula Óssea/metabolismoTetracloreto de Carbono/administração & dosagemMovimento CelularQuimeraColágeno Tipo I/metabolismoEtanol/administração & dosagemFemininoProteínas de Fluorescência Verde/metabolismoInflamaçãoFígado/metabolismoFígado/patologiaMasculinoCamundongosMetaloproteinase 9 da Matriz/metabolismoFagócitos/metabolismoInibidor Tecidual de Metaloproteinase-1/metabolismoFator de Crescimento Transformador beta/metabolismoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8572/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALOliveira SA Reduction of galectin-3....pdfOliveira SA Reduction of galectin-3....pdfapplication/pdf510816https://www.arca.fiocruz.br/bitstream/icict/8572/2/Oliveira%20SA%20Reduction%20of%20galectin-3....pdfce9cc2197c98efa3f557460a3a5f2bd1MD52TEXTOliveira SA Reduction of galectin-3....pdf.txtOliveira SA Reduction of galectin-3....pdf.txtExtracted texttext/plain43051https://www.arca.fiocruz.br/bitstream/icict/8572/3/Oliveira%20SA%20Reduction%20of%20galectin-3....pdf.txt79f65373d1e2d76ac503fe669f9c7e67MD53icict/85722023-03-15 14:33:24.489oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis.
title Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis.
spellingShingle Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis.
Oliveira, Sheilla Andrade de
Carbon tetrachloride
Galectin-3
Hepatic cirrhosis
Mice
Stem cells
Transplante de Medula Óssea/métodos
Galectina 3/metabolismo
Cirrose Hepática Experimental/terapia
Animais
Células da Medula Óssea/citologia
Células da Medula Óssea/metabolismo
Tetracloreto de Carbono/administração & dosagem
Movimento Celular
Quimera
Colágeno Tipo I/metabolismo
Etanol/administração & dosagem
Feminino
Proteínas de Fluorescência Verde/metabolismo
Inflamação
Fígado/metabolismo
Fígado/patologia
Masculino
Camundongos
Metaloproteinase 9 da Matriz/metabolismo
Fagócitos/metabolismo
Inibidor Tecidual de Metaloproteinase-1/metabolismo
Fator de Crescimento Transformador beta/metabolismo
title_short Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis.
title_full Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis.
title_fullStr Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis.
title_full_unstemmed Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis.
title_sort Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis.
author Oliveira, Sheilla Andrade de
author_facet Oliveira, Sheilla Andrade de
Souza, Bruno Solano de Freitas
Barreto, Elton Pereira Sá
Kaneto, Carla Martins
Almeida Neto, Hélio
Azevedo, Carine Machado
Guimarães, Elisalva Teixeira
Freitas, Luiz Antonio Rodrigues de
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
author_role author
author2 Souza, Bruno Solano de Freitas
Barreto, Elton Pereira Sá
Kaneto, Carla Martins
Almeida Neto, Hélio
Azevedo, Carine Machado
Guimarães, Elisalva Teixeira
Freitas, Luiz Antonio Rodrigues de
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Sheilla Andrade de
Souza, Bruno Solano de Freitas
Barreto, Elton Pereira Sá
Kaneto, Carla Martins
Almeida Neto, Hélio
Azevedo, Carine Machado
Guimarães, Elisalva Teixeira
Freitas, Luiz Antonio Rodrigues de
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
dc.subject.en.pt_BR.fl_str_mv Carbon tetrachloride
Galectin-3
Hepatic cirrhosis
Mice
Stem cells
topic Carbon tetrachloride
Galectin-3
Hepatic cirrhosis
Mice
Stem cells
Transplante de Medula Óssea/métodos
Galectina 3/metabolismo
Cirrose Hepática Experimental/terapia
Animais
Células da Medula Óssea/citologia
Células da Medula Óssea/metabolismo
Tetracloreto de Carbono/administração & dosagem
Movimento Celular
Quimera
Colágeno Tipo I/metabolismo
Etanol/administração & dosagem
Feminino
Proteínas de Fluorescência Verde/metabolismo
Inflamação
Fígado/metabolismo
Fígado/patologia
Masculino
Camundongos
Metaloproteinase 9 da Matriz/metabolismo
Fagócitos/metabolismo
Inibidor Tecidual de Metaloproteinase-1/metabolismo
Fator de Crescimento Transformador beta/metabolismo
dc.subject.decs.pt_BR.fl_str_mv Transplante de Medula Óssea/métodos
Galectina 3/metabolismo
Cirrose Hepática Experimental/terapia
Animais
Células da Medula Óssea/citologia
Células da Medula Óssea/metabolismo
Tetracloreto de Carbono/administração & dosagem
Movimento Celular
Quimera
Colágeno Tipo I/metabolismo
Etanol/administração & dosagem
Feminino
Proteínas de Fluorescência Verde/metabolismo
Inflamação
Fígado/metabolismo
Fígado/patologia
Masculino
Camundongos
Metaloproteinase 9 da Matriz/metabolismo
Fagócitos/metabolismo
Inibidor Tecidual de Metaloproteinase-1/metabolismo
Fator de Crescimento Transformador beta/metabolismo
description Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhaes. Recife, PE, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-10-09T19:21:46Z
dc.date.available.fl_str_mv 2014-10-09T19:21:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, S. A. et al. Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis. Cytotherapy, v. 14, n. 3, p. 339-349, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8572
dc.identifier.issn.none.fl_str_mv 1477-2566
dc.identifier.doi.none.fl_str_mv 10.3109/14653249.2011.637668
identifier_str_mv OLIVEIRA, S. A. et al. Reduction of galectin-3 expression and liver fibrosis after cell therapy in a mouse model of cirrhosis. Cytotherapy, v. 14, n. 3, p. 339-349, 2012.
1477-2566
10.3109/14653249.2011.637668
url https://www.arca.fiocruz.br/handle/icict/8572
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Informa Healthcare
publisher.none.fl_str_mv Informa Healthcare
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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